Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
Identifieur interne : 000168 ( Ncbi/Checkpoint ); précédent : 000167; suivant : 000169Isolation and characterization of a highly divergent HIV-2[GH-2]: generation of an infectious molecular clone and functional analysis of its rev-responsive element in response to primate retrovirus transactivators (Rev and Rex).
Auteurs : M. Kawamura [Japon] ; J. Katahira ; M. Fukasawa ; J. Sakuragi ; K. Ishikawa ; M. Nakai ; J A Mingle ; M. Osei-Kwasi ; V B Netty ; H. AkariSource :
- Virology [ 0042-6822 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Cartographie de restriction, Clonage moléculaire, Gènes pX, Gènes rev, Régulation de l'expression des gènes viraux, Répétition terminale longue du VIH, Similitude de séquences d'acides nucléiques, VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique), Variation génétique.
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Viral.
- genetics : HIV-2.
- Cloning, Molecular, Gene Expression Regulation, Viral, Genes, pX, Genes, rev, Genetic Variation, HIV Long Terminal Repeat, Restriction Mapping, Sequence Homology, Nucleic Acid.
Abstract
A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.
PubMed: 1585652
Affiliations:
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Kawamura</name><affiliation wicri:level="4"><nlm:affiliation>Institute for Virus Research, Kyoto University, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Institute for Virus Research, Kyoto University</wicri:regionArea><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Katahira, J" sort="Katahira, J" uniqKey="Katahira J" first="J" last="Katahira">J. Katahira</name></author><author><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M" last="Fukasawa">M. Fukasawa</name></author><author><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J" last="Sakuragi">J. Sakuragi</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Nakai, M" sort="Nakai, M" uniqKey="Nakai M" first="M" last="Nakai">M. Nakai</name></author><author><name sortKey="Mingle, J A" sort="Mingle, J A" uniqKey="Mingle J" first="J A" last="Mingle">J A Mingle</name></author><author><name sortKey="Osei Kwasi, M" sort="Osei Kwasi, M" uniqKey="Osei Kwasi M" first="M" last="Osei-Kwasi">M. Osei-Kwasi</name></author><author><name sortKey="Netty, V B" sort="Netty, V B" uniqKey="Netty V" first="V B" last="Netty">V B Netty</name></author><author><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H" last="Akari">H. Akari</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term><term>DNA, Viral (genetics)</term><term>Gene Expression Regulation, Viral</term><term>Genes, pX</term><term>Genes, rev</term><term>Genetic Variation</term><term>HIV Long Terminal Repeat</term><term>HIV-2 (genetics)</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Gènes pX</term><term>Gènes rev</term><term>Régulation de l'expression des gènes viraux</term><term>Répétition terminale longue du VIH</term><term>Similitude de séquences d'acides nucléiques</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique)</term><term>Variation génétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-2</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Genes, pX</term><term>Genes, rev</term><term>Genetic Variation</term><term>HIV Long Terminal Repeat</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Clonage moléculaire</term><term>Gènes pX</term><term>Gènes rev</term><term>Régulation de l'expression des gènes viraux</term><term>Répétition terminale longue du VIH</term><term>Similitude de séquences d'acides nucléiques</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A highly divergent HIV-2 designated as HIV-2[GH-2] was obtained from an AIDS-related complex (ARC) patient in Ghana. A full-length molecular clone of this isolate was obtained and a biologically active clone was constructed. Its restriction pattern differed from that of prototype HIV-2[GH-1] in 25 of 35 restriction sites, but was strikingly similar to a previously characterized HIV-2 isolate from a Ghanaian (HIV-2ALT). The conserved integrase region (288-bp fragment) previously displayed 95% identity with that of ALT but 17-20% divergence from the HIV-2 prototype member, and a new distinct subgroup (HIV-2b) of HIV-2 consisting of GH-2 and ALT was postulated (Miura et al. 1991.) These isolates, however, were biologically distinguishable from each other by its replication capacity in a monocyte line, U937, in which GH-2 could not grow but ALT grew well. In addition, the nucleotide sequence of the LTR of this new isolate displays 21% divergence from that of prototype HIV-2[GH-1], but the core enhancer, Sp1 binding sites and TATA box were conserved. Although the 3' half of the env gene sequence which is deleted in HIV-2ALT clone showed 27% diversity from the prototype, functional differences in the rev-responsive element were not observed.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><noCountry><name sortKey="Akari, H" sort="Akari, H" uniqKey="Akari H" first="H" last="Akari">H. Akari</name><name sortKey="Fukasawa, M" sort="Fukasawa, M" uniqKey="Fukasawa M" first="M" last="Fukasawa">M. Fukasawa</name><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name><name sortKey="Katahira, J" sort="Katahira, J" uniqKey="Katahira J" first="J" last="Katahira">J. Katahira</name><name sortKey="Mingle, J A" sort="Mingle, J A" uniqKey="Mingle J" first="J A" last="Mingle">J A Mingle</name><name sortKey="Nakai, M" sort="Nakai, M" uniqKey="Nakai M" first="M" last="Nakai">M. Nakai</name><name sortKey="Netty, V B" sort="Netty, V B" uniqKey="Netty V" first="V B" last="Netty">V B Netty</name><name sortKey="Osei Kwasi, M" sort="Osei Kwasi, M" uniqKey="Osei Kwasi M" first="M" last="Osei-Kwasi">M. Osei-Kwasi</name><name sortKey="Sakuragi, J" sort="Sakuragi, J" uniqKey="Sakuragi J" first="J" last="Sakuragi">J. Sakuragi</name></noCountry><country name="Japon"><region name="Région du Kansai"><name sortKey="Kawamura, M" sort="Kawamura, M" uniqKey="Kawamura M" first="M" last="Kawamura">M. Kawamura</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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