Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
Identifieur interne : 001200 ( Main/Merge ); précédent : 001199; suivant : 001201Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
Auteurs : Mikako Takahoko [Japon] ; Minoru Tobiume [Japon] ; Koichi Ishikawa [Ghana] ; William Ampofo [Ghana] ; Naoki Yamamoto [Japon] ; Michiyuki Matsuda [Japon] ; Masashi Tatsumi [Japon]Source :
- AIDS research and human retroviruses [ 0889-2229 ] ; 2001.
Descripteurs français
- Pascal (Inist)
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
Abstract
We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
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- to stream PascalFrancis, to step Curation: 000014
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Pascal:02-0322075Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells</title>
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<author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name>
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<author><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name>
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<s2>Tokyo 113-8519</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
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<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
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<author><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1>
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<s3>JPN</s3>
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<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
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<author><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1>
<s2>Tokyo 162-8640</s2>
<s3>JPN</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
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<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
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<series><title level="j" type="main">AIDS research and human retroviruses</title>
<title level="j" type="abbreviated">AIDS res. hum. retroviruses</title>
<idno type="ISSN">0889-2229</idno>
<imprint><date when="2001">2001</date>
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<seriesStmt><title level="j" type="main">AIDS research and human retroviruses</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blood</term>
<term>Blood cell</term>
<term>Breakpoint</term>
<term>CCR5 chemokine receptor</term>
<term>Ghana</term>
<term>HIV-1 virus</term>
<term>Infection</term>
<term>Isolate</term>
<term>Phylogeny</term>
<term>Phytohemagglutinin</term>
<term>Recombination</term>
<term>Replication</term>
<term>Transfection</term>
<term>Use</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term>
<term>Cellule sanguine</term>
<term>Sang</term>
<term>Isolat</term>
<term>Ghana</term>
<term>Phylogenèse</term>
<term>Recombinaison</term>
<term>Point cassure</term>
<term>Transfection</term>
<term>Infection</term>
<term>Récepteur chimiokine CCR5</term>
<term>Utilisation</term>
<term>Phytohémagglutinine</term>
<term>Réplication</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term>
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<front><div type="abstract" xml:lang="en">We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</div>
</front>
</TEI>
<affiliations><list><country><li>Ghana</li>
<li>Japon</li>
</country>
<region><li>Région de Kantō</li>
<li>Région du Grand Accra</li>
</region>
<settlement><li>Accra</li>
<li>Legon (Ghana)</li>
<li>Tokyo</li>
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<orgName><li>Université du Ghana</li>
</orgName>
</list>
<tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Takahoko, Mikako" sort="Takahoko, Mikako" uniqKey="Takahoko M" first="Mikako" last="Takahoko">Mikako Takahoko</name>
</region>
<name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name>
<name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name>
<name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name>
<name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name>
</country>
<country name="Ghana"><region name="Région du Grand Accra"><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name>
</region>
<name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name>
</country>
</tree>
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</record>
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