Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
Identifieur interne : 000116 ( PascalFrancis/Checkpoint ); précédent : 000115; suivant : 000117Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
Auteurs : Mikako Takahoko [Japon] ; Minoru Tobiume [Japon] ; Koichi Ishikawa [Ghana] ; William Ampofo [Ghana] ; Naoki Yamamoto [Japon] ; Michiyuki Matsuda [Japon] ; Masashi Tatsumi [Japon]Source :
- AIDS research and human retroviruses [ 0889-2229 ] ; 2001.
Descripteurs français
- Pascal (Inist)
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
Abstract
We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells</title><author><name sortKey="Takahoko, Mikako" sort="Takahoko, Mikako" uniqKey="Takahoko M" first="Mikako" last="Takahoko">Mikako Takahoko</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Microbiology, Tokyo Medical and Dental University</s1><s2>Tokyo 113-8519</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1><s2>Tokyo 162-8640</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de 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International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Microbiology, Tokyo Medical and Dental University</s1><s2>Tokyo 113-8519</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1><s2>Tokyo 162-8640</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j" type="main">AIDS research and human retroviruses</title><title level="j" type="abbreviated">AIDS res. hum. retroviruses</title><idno type="ISSN">0889-2229</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">AIDS research and human retroviruses</title><title level="j" type="abbreviated">AIDS res. hum. retroviruses</title><idno type="ISSN">0889-2229</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blood</term><term>Blood cell</term><term>Breakpoint</term><term>CCR5 chemokine receptor</term><term>Ghana</term><term>HIV-1 virus</term><term>Infection</term><term>Isolate</term><term>Phylogeny</term><term>Phytohemagglutinin</term><term>Recombination</term><term>Replication</term><term>Transfection</term><term>Use</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term><term>Cellule sanguine</term><term>Sang</term><term>Isolat</term><term>Ghana</term><term>Phylogenèse</term><term>Recombinaison</term><term>Point cassure</term><term>Transfection</term><term>Infection</term><term>Récepteur chimiokine CCR5</term><term>Utilisation</term><term>Phytohémagglutinine</term><term>Réplication</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0889-2229</s0></fA01><fA02 i1="01"><s0>ARHRE7</s0></fA02><fA03 i2="1"><s0>AIDS res. hum. retroviruses</s0></fA03><fA05><s2>17</s2></fA05><fA06><s2>11</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells</s1></fA08><fA11 i1="01" i2="1"><s1>TAKAHOKO (Mikako)</s1></fA11><fA11 i1="02" i2="1"><s1>TOBIUME (Minoru)</s1></fA11><fA11 i1="03" i2="1"><s1>ISHIKAWA (Koichi)</s1></fA11><fA11 i1="04" i2="1"><s1>AMPOFO (William)</s1></fA11><fA11 i1="05" i2="1"><s1>YAMAMOTO (Naoki)</s1></fA11><fA11 i1="06" i2="1"><s1>MATSUDA (Michiyuki)</s1></fA11><fA11 i1="07" i2="1"><s1>TATSUMI (Masashi)</s1></fA11><fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="03"><s1>Department of Microbiology, Tokyo Medical and Dental University</s1><s2>Tokyo 113-8519</s2><s3>JPN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1><s2>Tokyo 162-8640</s2><s3>JPN</s3><sZ>7 aut.</sZ></fA14><fA20><s1>1083-1087</s1></fA20><fA21><s1>2001</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>20934</s2><s5>354000099074040120</s5></fA43><fA44><s0>0000</s0><s1>© 2002 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>12 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>02-0322075</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>AIDS research and human retroviruses</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</s0></fC01><fC02 i1="01" i2="X"><s0>002B06D01</s0></fC02><fC02 i1="02" i2="X"><s0>002A05C04</s0></fC02><fC02 i1="03" i2="X"><s0>002A05</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Virus HIV1</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>HIV-1 virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>HIV-1 virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Cellule sanguine</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Blood cell</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Célula sanguínea</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Sang</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Blood</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Sangre</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Isolat</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" 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l="ENG"><s0>Phytohemagglutinin</s0><s2>NK</s2><s2>FR</s2><s5>69</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Fitohemaglutinina</s0><s2>NK</s2><s2>FR</s2><s5>69</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Réplication</s0><s5>70</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Replication</s0><s5>70</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Replicación</s0><s5>70</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virus immunodéficience humaine</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Human immunodeficiency virus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Human immunodeficiency virus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Lentivirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Lentivirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Lentivirus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Retroviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Retroviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Retroviridae</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Afrique</s0><s2>NG</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Africa</s0><s2>NG</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Africa</s0><s2>NG</s2></fC07><fN21><s1>182</s1></fN21><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Ghana</li><li>Japon</li></country><region><li>Région de Kantō</li><li>Région du Grand Accra</li></region><settlement><li>Accra</li><li>Legon (Ghana)</li><li>Tokyo</li></settlement><orgName><li>Université du Ghana</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Takahoko, Mikako" sort="Takahoko, Mikako" uniqKey="Takahoko M" first="Mikako" last="Takahoko">Mikako Takahoko</name></region><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></country><country name="Ghana"><region name="Région du Grand Accra"><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name></region><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= PascalFrancis
|étape= Checkpoint
|type= RBID
|clé= Pascal:02-0322075
|texte= Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
}}
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