SidaGhanaV1, PascalFrancis, Corpus, bibRecord, 000134

Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells

Identifieur interne : 000134 ( PascalFrancis/Corpus ); précédent : 000133; suivant : 000135

Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells

Auteurs : Mikako Takahoko ; Minoru Tobiume ; Koichi Ishikawa ; William Ampofo ; Naoki Yamamoto ; Michiyuki Matsuda ; Masashi Tatsumi

Source :

AIDS research and human retroviruses [ 0889-2229 ] ; 2001.

RBID : Pascal:02-0322075

Descripteurs français

Pascal (Inist)
- Virus HIV1, Cellule sanguine, Sang, Isolat, Ghana, Phylogenèse, Recombinaison, Point cassure, Transfection, Infection, Récepteur chimiokine CCR5, Utilisation, Phytohémagglutinine, Réplication.

English descriptors

KwdEn :
- Blood, Blood cell, Breakpoint, CCR5 chemokine receptor, Ghana, HIV-1 virus, Infection, Isolate, Phylogeny, Phytohemagglutinin, Recombination, Replication, Transfection, Use.

Abstract

We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A02	`01`			`@0 ARHRE7`
A03		`1`		`@0 AIDS res. hum. retroviruses`
A05				`@2 17`
A06				`@2 11`
A08	`01`	`1`	`ENG`	`@1 Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells`
A11	`01`	`1`		`@1 TAKAHOKO (Mikako)`
A11	`02`	`1`		`@1 TOBIUME (Minoru)`
A11	`03`	`1`		`@1 ISHIKAWA (Koichi)`
A11	`04`	`1`		`@1 AMPOFO (William)`
A11	`05`	`1`		`@1 YAMAMOTO (Naoki)`
A11	`06`	`1`		`@1 MATSUDA (Michiyuki)`
A11	`07`	`1`		`@1 TATSUMI (Masashi)`
A14	`01`			`@1 Department of Pathology, Research Institute, International Medical Center of Japan @2 Tokyo 162-8644 @3 JPN @Z 1 aut. @Z 2 aut. @Z 6 aut.`
A14	`02`			`@1 Noguchi Memorial Institute for Medical Research, University of Ghana @2 Legon @3 GHA @Z 3 aut. @Z 4 aut.`
A14	`03`			`@1 Department of Microbiology, Tokyo Medical and Dental University @2 Tokyo 113-8519 @3 JPN @Z 5 aut.`
A14	`04`			`@1 Department of Veterinary Science, National Institute of Infectious Diseases @2 Tokyo 162-8640 @3 JPN @Z 7 aut.`
A20				`@1 1083-1087`
A21				`@1 2001`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 20934 @5 354000099074040120`
A44				`@0 0000 @1 © 2002 INIST-CNRS. All rights reserved.`
A45				`@0 12 ref.`
A47	`01`	`1`		`@0 02-0322075`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 AIDS research and human retroviruses`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
C02	`01`	`X`		`@0 002B06D01`
C02	`02`	`X`		`@0 002A05C04`
C02	`03`	`X`		`@0 002A05`
C03	`01`	`X`	`FRE`	`@0 Virus HIV1 @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 HIV-1 virus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 HIV-1 virus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Cellule sanguine @5 05`
C03	`02`	`X`	`ENG`	`@0 Blood cell @5 05`
C03	`02`	`X`	`SPA`	`@0 Célula sanguínea @5 05`
C03	`03`	`X`	`FRE`	`@0 Sang @5 06`
C03	`03`	`X`	`ENG`	`@0 Blood @5 06`
C03	`03`	`X`	`SPA`	`@0 Sangre @5 06`
C03	`04`	`X`	`FRE`	`@0 Isolat @5 07`
C03	`04`	`X`	`ENG`	`@0 Isolate @5 07`
C03	`04`	`X`	`SPA`	`@0 Aislado @5 07`
C03	`05`	`X`	`FRE`	`@0 Ghana @2 NG @5 08`
C03	`05`	`X`	`ENG`	`@0 Ghana @2 NG @5 08`
C03	`05`	`X`	`SPA`	`@0 Ghana @2 NG @5 08`
C03	`06`	`X`	`FRE`	`@0 Phylogenèse @5 09`
C03	`06`	`X`	`ENG`	`@0 Phylogeny @5 09`
C03	`06`	`X`	`SPA`	`@0 Filogénesis @5 09`
C03	`07`	`X`	`FRE`	`@0 Recombinaison @5 10`
C03	`07`	`X`	`ENG`	`@0 Recombination @5 10`
C03	`07`	`X`	`SPA`	`@0 Recombinación @5 10`
C03	`08`	`X`	`FRE`	`@0 Point cassure @5 11`
C03	`08`	`X`	`ENG`	`@0 Breakpoint @5 11`
C03	`08`	`X`	`SPA`	`@0 Punto ruptura @5 11`
C03	`09`	`X`	`FRE`	`@0 Transfection @5 12`
C03	`09`	`X`	`ENG`	`@0 Transfection @5 12`
C03	`09`	`X`	`SPA`	`@0 Transfección @5 12`
C03	`10`	`X`	`FRE`	`@0 Infection @5 14`
C03	`10`	`X`	`ENG`	`@0 Infection @5 14`
C03	`10`	`X`	`SPA`	`@0 Infección @5 14`
C03	`11`	`X`	`FRE`	`@0 Récepteur chimiokine CCR5 @5 67`
C03	`11`	`X`	`ENG`	`@0 CCR5 chemokine receptor @5 67`
C03	`11`	`X`	`SPA`	`@0 Receptor quimioquina CCR5 @5 67`
C03	`12`	`X`	`FRE`	`@0 Utilisation @5 68`
C03	`12`	`X`	`ENG`	`@0 Use @5 68`
C03	`12`	`X`	`SPA`	`@0 Utilización @5 68`
C03	`13`	`X`	`FRE`	`@0 Phytohémagglutinine @2 NK @2 FR @5 69`
C03	`13`	`X`	`ENG`	`@0 Phytohemagglutinin @2 NK @2 FR @5 69`
C03	`13`	`X`	`SPA`	`@0 Fitohemaglutinina @2 NK @2 FR @5 69`
C03	`14`	`X`	`FRE`	`@0 Réplication @5 70`
C03	`14`	`X`	`ENG`	`@0 Replication @5 70`
C03	`14`	`X`	`SPA`	`@0 Replicación @5 70`
C07	`01`	`X`	`FRE`	`@0 Virus immunodéficience humaine @2 NW`
C07	`01`	`X`	`ENG`	`@0 Human immunodeficiency virus @2 NW`
C07	`01`	`X`	`SPA`	`@0 Human immunodeficiency virus @2 NW`
C07	`02`	`X`	`FRE`	`@0 Lentivirus @2 NW`
C07	`02`	`X`	`ENG`	`@0 Lentivirus @2 NW`
C07	`02`	`X`	`SPA`	`@0 Lentivirus @2 NW`
C07	`03`	`X`	`FRE`	`@0 Retroviridae @2 NW`
C07	`03`	`X`	`ENG`	`@0 Retroviridae @2 NW`
C07	`03`	`X`	`SPA`	`@0 Retroviridae @2 NW`
C07	`04`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`04`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`04`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`05`	`X`	`FRE`	`@0 Afrique @2 NG`
C07	`05`	`X`	`ENG`	`@0 Africa @2 NG`
C07	`05`	`X`	`SPA`	`@0 Africa @2 NG`
N21				`@1 182`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 02-0322075 INIST
ET :	Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
AU :	TAKAHOKO (Mikako); TOBIUME (Minoru); ISHIKAWA (Koichi); AMPOFO (William); YAMAMOTO (Naoki); MATSUDA (Michiyuki); TATSUMI (Masashi)
AF :	Department of Pathology, Research Institute, International Medical Center of Japan/Tokyo 162-8644/Japon (1 aut., 2 aut., 6 aut.); Noguchi Memorial Institute for Medical Research, University of Ghana/Legon/Ghana (3 aut., 4 aut.); Department of Microbiology, Tokyo Medical and Dental University/Tokyo 113-8519/Japon (5 aut.); Department of Veterinary Science, National Institute of Infectious Diseases/Tokyo 162-8640/Japon (7 aut.)
DT :	Publication en série; Niveau analytique
SO :	AIDS research and human retroviruses; ISSN 0889-2229; Coden ARHRE7; Etats-Unis; Da. 2001; Vol. 17; No. 11; Pp. 1083-1087; Bibl. 12 ref.
LA :	Anglais
EA :	We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
CC :	002B06D01; 002A05C04; 002A05
FD :	Virus HIV1; Cellule sanguine; Sang; Isolat; Ghana; Phylogenèse; Recombinaison; Point cassure; Transfection; Infection; Récepteur chimiokine CCR5; Utilisation; Phytohémagglutinine; Réplication
FG :	Virus immunodéficience humaine; Lentivirus; Retroviridae; Virus; Afrique
ED :	HIV-1 virus; Blood cell; Blood; Isolate; Ghana; Phylogeny; Recombination; Breakpoint; Transfection; Infection; CCR5 chemokine receptor; Use; Phytohemagglutinin; Replication
EG :	Human immunodeficiency virus; Lentivirus; Retroviridae; Virus; Africa
SD :	HIV-1 virus; Célula sanguínea; Sangre; Aislado; Ghana; Filogénesis; Recombinación; Punto ruptura; Transfección; Infección; Receptor quimioquina CCR5; Utilización; Fitohemaglutinina; Replicación
LO :	INIST-20934.354000099074040120
ID :	02-0322075

Links to Exploration step

Pascal:02-0322075

Le document en format XML

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<author><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name>
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<author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name>
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<author><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name>
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<term>Blood cell</term>
<term>Breakpoint</term>
<term>CCR5 chemokine receptor</term>
<term>Ghana</term>
<term>HIV-1 virus</term>
<term>Infection</term>
<term>Isolate</term>
<term>Phylogeny</term>
<term>Phytohemagglutinin</term>
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<term>Recombinaison</term>
<term>Point cassure</term>
<term>Transfection</term>
<term>Infection</term>
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<front><div type="abstract" xml:lang="en">We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</div>
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<fA11 i1="01" i2="1"><s1>TAKAHOKO (Mikako)</s1>
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<fA11 i1="07" i2="1"><s1>TATSUMI (Masashi)</s1>
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<fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1>
<s2>Tokyo 162-8644</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1>
<s2>Legon</s2>
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</fA14>
<fA14 i1="03"><s1>Department of Microbiology, Tokyo Medical and Dental University</s1>
<s2>Tokyo 113-8519</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1>
<s2>Tokyo 162-8640</s2>
<s3>JPN</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>1083-1087</s1>
</fA20>
<fA21><s1>2001</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
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<s1>© 2002 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>12 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>02-0322075</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>AIDS research and human retroviruses</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B06D01</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A05C04</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>002A05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Virus HIV1</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>HIV-1 virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>HIV-1 virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Cellule sanguine</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Blood cell</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Célula sanguínea</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Sang</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Blood</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Sangre</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Isolat</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Isolate</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Aislado</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Ghana</s0>
<s2>NG</s2>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Ghana</s0>
<s2>NG</s2>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Ghana</s0>
<s2>NG</s2>
<s5>08</s5>
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<fC03 i1="06" i2="X" l="FRE"><s0>Phylogenèse</s0>
<s5>09</s5>
</fC03>
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<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Filogénesis</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Recombinaison</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Recombination</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Recombinación</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Point cassure</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Breakpoint</s0>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Punto ruptura</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Transfection</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Transfection</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Transfección</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Infection</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Infection</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Infección</s0>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Récepteur chimiokine CCR5</s0>
<s5>67</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>CCR5 chemokine receptor</s0>
<s5>67</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Receptor quimioquina CCR5</s0>
<s5>67</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Utilisation</s0>
<s5>68</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Use</s0>
<s5>68</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Utilización</s0>
<s5>68</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Phytohémagglutinine</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>69</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Phytohemagglutinin</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>69</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Fitohemaglutinina</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>69</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Réplication</s0>
<s5>70</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Replication</s0>
<s5>70</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Replicación</s0>
<s5>70</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Virus immunodéficience humaine</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Human immunodeficiency virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Human immunodeficiency virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Lentivirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Lentivirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Lentivirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Retroviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Retroviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Retroviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Afrique</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Africa</s0>
<s2>NG</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Africa</s0>
<s2>NG</s2>
</fC07>
<fN21><s1>182</s1>
</fN21>
<fN82><s1>OTO</s1>
</fN82>
</pA>
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<server><NO>PASCAL 02-0322075 INIST</NO>
<ET>Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells</ET>
<AU>TAKAHOKO (Mikako); TOBIUME (Minoru); ISHIKAWA (Koichi); AMPOFO (William); YAMAMOTO (Naoki); MATSUDA (Michiyuki); TATSUMI (Masashi)</AU>
<AF>Department of Pathology, Research Institute, International Medical Center of Japan/Tokyo 162-8644/Japon (1 aut., 2 aut., 6 aut.); Noguchi Memorial Institute for Medical Research, University of Ghana/Legon/Ghana (3 aut., 4 aut.); Department of Microbiology, Tokyo Medical and Dental University/Tokyo 113-8519/Japon (5 aut.); Department of Veterinary Science, National Institute of Infectious Diseases/Tokyo 162-8640/Japon (7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>AIDS research and human retroviruses; ISSN 0889-2229; Coden ARHRE7; Etats-Unis; Da. 2001; Vol. 17; No. 11; Pp. 1083-1087; Bibl. 12 ref.</SO>
<LA>Anglais</LA>
<EA>We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</EA>
<CC>002B06D01; 002A05C04; 002A05</CC>
<FD>Virus HIV1; Cellule sanguine; Sang; Isolat; Ghana; Phylogenèse; Recombinaison; Point cassure; Transfection; Infection; Récepteur chimiokine CCR5; Utilisation; Phytohémagglutinine; Réplication</FD>
<FG>Virus immunodéficience humaine; Lentivirus; Retroviridae; Virus; Afrique</FG>
<ED>HIV-1 virus; Blood cell; Blood; Isolate; Ghana; Phylogeny; Recombination; Breakpoint; Transfection; Infection; CCR5 chemokine receptor; Use; Phytohemagglutinin; Replication</ED>
<EG>Human immunodeficiency virus; Lentivirus; Retroviridae; Virus; Africa</EG>
<SD>HIV-1 virus; Célula sanguínea; Sangre; Aislado; Ghana; Filogénesis; Recombinación; Punto ruptura; Transfección; Infección; Receptor quimioquina CCR5; Utilización; Fitohemaglutinina; Replicación</SD>
<LO>INIST-20934.354000099074040120</LO>
<ID>02-0322075</ID>
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	Le SIDA au Ghana (serveur d'exploration)
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Le SIDA au Ghana (serveur d'exploration)

Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells

Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells

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