In vitro study of GDNF release from biodegradable PLGA microspheres
Identifieur interne : 000726 ( PascalFrancis/Curation ); précédent : 000725; suivant : 000727In vitro study of GDNF release from biodegradable PLGA microspheres
Auteurs : Anne Aubert-Pouëssel [France] ; Marie-Claire Venier-Julienne [France] ; Anne Clavreul [France] ; Michelle Sergent [France] ; Christophe Jollivet [France] ; Claudia N. Montero-Menei [France] ; Emmanuel Garcion [France] ; David C. Bibby [France] ; Philippe Menei [France] ; Jean-Pierre Benoit [France]Source :
- Journal of controlled release [ 0168-3659 ] ; 2004.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Biodégradabilité.
English descriptors
- KwdEn :
Abstract
Glial cell line-derived neurotrophic factor (GDNF) is a protein with potent trophic actions on dopaminergic neurons, which is under investigation as a therapeutic agent for the treatment of neurodegenerative disorders, including Parkinson's disease. The aim of this work was to develop GDNF-loaded microspheres, which could be implanted by stereotaxy in the brain and could offer an alternative strategy in the treatment of Parkinson's disease. Aw/o/w extraction-evaporation technique was chosen to prepare protein-loaded microspheres. An in vitro release study of the protein was required to assess the retention of integrity and the performance of the microsphere formulation with regard to sustained release. In order to assess the in vitro release profile of the GDNF-loaded microspheres, a preliminary study was performed to select an appropriate buffer for GDNF stabilization, using experimental designs. GDNF was measured by both enzyme-linked immunosorbant assay (ELISA) and radioactivity using 125I-GDNF. The GDNF-loaded microsphere release profile was assessed in a low continuous flow system, and showed a sustained release over 56 days of biologically active GDNF at clinically relevant doses.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">In vitro study of GDNF release from biodegradable PLGA microspheres</title><author><name sortKey="Aubert Pouessel, Anne" sort="Aubert Pouessel, Anne" uniqKey="Aubert Pouessel A" first="Anne" last="Aubert-Pouëssel">Anne Aubert-Pouëssel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Venier Julienne, Marie Claire" sort="Venier Julienne, Marie Claire" uniqKey="Venier Julienne M" first="Marie-Claire" last="Venier-Julienne">Marie-Claire Venier-Julienne</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Clavreul, Anne" sort="Clavreul, Anne" uniqKey="Clavreul A" first="Anne" last="Clavreul">Anne Clavreul</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Service tie Neurochirurgie, Centre Hospitalier Universitaire d'Angers</s1><s2>49033, Angers</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Sergent, Michelle" sort="Sergent, Michelle" uniqKey="Sergent M" first="Michelle" last="Sergent">Michelle Sergent</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratoire de Méthodologie de la Recherche Expérimentale, Faculté des Sciences et Techniques de Saint Jérôme, avenue Escadrille Normandie-Niemen</s1><s2>13 397, Marseille</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Jollivet, Christophe" sort="Jollivet, Christophe" uniqKey="Jollivet C" first="Christophe" last="Jollivet">Christophe Jollivet</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Montero Menei, Claudia N" sort="Montero Menei, Claudia N" uniqKey="Montero Menei C" first="Claudia N." last="Montero-Menei">Claudia N. 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Montero-Menei</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Garcion, Emmanuel" sort="Garcion, Emmanuel" uniqKey="Garcion E" first="Emmanuel" last="Garcion">Emmanuel Garcion</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Service tie Neurochirurgie, Centre Hospitalier Universitaire d'Angers</s1><s2>49033, Angers</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Bibby, David C" sort="Bibby, David C" uniqKey="Bibby D" first="David C." last="Bibby">David C. Bibby</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Menei, Philippe" sort="Menei, Philippe" uniqKey="Menei P" first="Philippe" last="Menei">Philippe Menei</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Service tie Neurochirurgie, Centre Hospitalier Universitaire d'Angers</s1><s2>49033, Angers</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country></affiliation></author><author><name sortKey="Benoit, Jean Pierre" sort="Benoit, Jean Pierre" uniqKey="Benoit J" first="Jean-Pierre" last="Benoit">Jean-Pierre Benoit</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>France</country></affiliation></author></analytic><series><title level="j" type="main">Journal of controlled release</title><title level="j" type="abbreviated">J. control. release</title><idno type="ISSN">0168-3659</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of controlled release</title><title level="j" type="abbreviated">J. control. release</title><idno type="ISSN">0168-3659</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradability</term><term>Copolymer</term><term>Experimental design</term><term>Glycolic acid</term><term>Glycolic acid polymer</term><term>In vitro</term><term>Lactic acid</term><term>Lactic acid polymer</term><term>Microsphere</term><term>Pharmaceutical technology</term><term>Release</term><term>Stabilization</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>In vitro</term><term>Libération</term><term>Biodégradabilité</term><term>Lactique acide polymère</term><term>Glycolique acide polymère</term><term>Copolymère</term><term>Microsphère</term><term>Lactique acide</term><term>Glycolique acide</term><term>Stabilisation</term><term>Plan expérience</term><term>Technologie pharmaceutique</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Biodégradabilité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glial cell line-derived neurotrophic factor (GDNF) is a protein with potent trophic actions on dopaminergic neurons, which is under investigation as a therapeutic agent for the treatment of neurodegenerative disorders, including Parkinson's disease. The aim of this work was to develop GDNF-loaded microspheres, which could be implanted by stereotaxy in the brain and could offer an alternative strategy in the treatment of Parkinson's disease. Aw/o/w extraction-evaporation technique was chosen to prepare protein-loaded microspheres. An in vitro release study of the protein was required to assess the retention of integrity and the performance of the microsphere formulation with regard to sustained release. In order to assess the in vitro release profile of the GDNF-loaded microspheres, a preliminary study was performed to select an appropriate buffer for GDNF stabilization, using experimental designs. GDNF was measured by both enzyme-linked immunosorbant assay (ELISA) and radioactivity using <sup>125</sup>I-GDNF. The GDNF-loaded microsphere release profile was assessed in a low continuous flow system, and showed a sustained release over 56 days of biologically active GDNF at clinically relevant doses.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0168-3659</s0></fA01><fA02 i1="01"><s0>JCREEC</s0></fA02><fA03 i2="1"><s0>J. control. release</s0></fA03><fA05><s2>95</s2></fA05><fA06><s2>3</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>In vitro study of GDNF release from biodegradable PLGA microspheres</s1></fA08><fA11 i1="01" i2="1"><s1>AUBERT-POUËSSEL (Anne)</s1></fA11><fA11 i1="02" i2="1"><s1>VENIER-JULIENNE (Marie-Claire)</s1></fA11><fA11 i1="03" i2="1"><s1>CLAVREUL (Anne)</s1></fA11><fA11 i1="04" i2="1"><s1>SERGENT (Michelle)</s1></fA11><fA11 i1="05" i2="1"><s1>JOLLIVET (Christophe)</s1></fA11><fA11 i1="06" i2="1"><s1>MONTERO-MENEI (Claudia N.)</s1></fA11><fA11 i1="07" i2="1"><s1>GARCION (Emmanuel)</s1></fA11><fA11 i1="08" i2="1"><s1>BIBBY (David C.)</s1></fA11><fA11 i1="09" i2="1"><s1>MENEI (Philippe)</s1></fA11><fA11 i1="10" i2="1"><s1>BENOIT (Jean-Pierre)</s1></fA11><fA14 i1="01"><s1>INSERM ERIT-M 0104, Ingénierie de la Vectorisation Particulaire, IBT, 10, Rue André Boquel</s1><s2>49 100, Angers</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>8 aut.</sZ><sZ>10 aut.</sZ></fA14><fA14 i1="02"><s1>Service tie Neurochirurgie, Centre Hospitalier Universitaire d'Angers</s1><s2>49033, Angers</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="03"><s1>Laboratoire de Méthodologie de la Recherche Expérimentale, Faculté des Sciences et Techniques de Saint Jérôme, avenue Escadrille Normandie-Niemen</s1><s2>13 397, Marseille</s2><s3>FRA</s3><sZ>4 aut.</sZ></fA14><fA20><s1>463-475</s1></fA20><fA21><s1>2004</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>20704</s2><s5>354000116973570100</s5></fA43><fA44><s0>0000</s0><s1>© 2005 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>48 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>05-0155607</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of controlled release</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>Glial cell line-derived neurotrophic factor (GDNF) is a protein with potent trophic actions on dopaminergic neurons, which is under investigation as a therapeutic agent for the treatment of neurodegenerative disorders, including Parkinson's disease. The aim of this work was to develop GDNF-loaded microspheres, which could be implanted by stereotaxy in the brain and could offer an alternative strategy in the treatment of Parkinson's disease. Aw/o/w extraction-evaporation technique was chosen to prepare protein-loaded microspheres. An in vitro release study of the protein was required to assess the retention of integrity and the performance of the microsphere formulation with regard to sustained release. In order to assess the in vitro release profile of the GDNF-loaded microspheres, a preliminary study was performed to select an appropriate buffer for GDNF stabilization, using experimental designs. GDNF was measured by both enzyme-linked immunosorbant assay (ELISA) and radioactivity using <sup>125</sup>I-GDNF. The GDNF-loaded microsphere release profile was assessed in a low continuous flow system, and showed a sustained release over 56 days of biologically active GDNF at clinically relevant doses.</s0></fC01><fC02 i1="01" i2="X"><s0>002B02A03</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>In vitro</s0><s5>11</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>In vitro</s0><s5>11</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>In vitro</s0><s5>11</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Libération</s0><s5>12</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Release</s0><s5>12</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Liberación</s0><s5>12</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Biodégradabilité</s0><s5>14</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Biodegradability</s0><s5>14</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Biodegradabilidad</s0><s5>14</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Lactique acide polymère</s0><s2>NK</s2><s5>15</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Lactic acid polymer</s0><s2>NK</s2><s5>15</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Láctico ácido polímero</s0><s2>NK</s2><s5>15</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Glycolique acide polymère</s0><s2>NK</s2><s5>16</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Glycolic acid polymer</s0><s2>NK</s2><s5>16</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Glicólico ácido polímero</s0><s2>NK</s2><s5>16</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Copolymère</s0><s2>NK</s2><s5>17</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Copolymer</s0><s2>NK</s2><s5>17</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Copolímero</s0><s2>NK</s2><s5>17</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Microsphère</s0><s5>18</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Microsphere</s0><s5>18</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Microsfera</s0><s5>18</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Lactique acide</s0><s2>NK</s2><s5>19</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Lactic acid</s0><s2>NK</s2><s5>19</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Láctico ácido</s0><s2>NK</s2><s5>19</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Glycolique acide</s0><s2>NK</s2><s2>FR</s2><s5>20</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Glycolic acid</s0><s2>NK</s2><s2>FR</s2><s5>20</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Glicolico ácido</s0><s2>NK</s2><s2>FR</s2><s5>20</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Stabilisation</s0><s5>21</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Stabilization</s0><s5>21</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Estabilización</s0><s5>21</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Plan expérience</s0><s5>22</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Experimental design</s0><s5>22</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Plan experiencia</s0><s5>22</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Technologie pharmaceutique</s0><s5>23</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Pharmaceutical technology</s0><s5>23</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Tecnología farmacéutica</s0><s5>23</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Hydroxyacide</s0><s5>61</s5></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Hydroxyacid</s0><s5>61</s5></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Hidroxiácido</s0><s5>61</s5></fC07><fN21><s1>101</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
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