Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity
Identifieur interne : 000554 ( Istex/Curation ); précédent : 000553; suivant : 000555Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity
Auteurs : Montserrat Cortina-Puig ; Anastasia C. H. Scangas [États-Unis] ; Zachary S. Marchese [États-Unis] ; Silvana Andreescu [États-Unis] ; Jean-Louis Marty ; Carole Calas-BlanchardSource :
- Electroanalysis [ 1040-0397 ] ; 2010-10.
English descriptors
Abstract
This paper describes the development of a xanthine oxidase/poly‐m‐phenylenediamine (XOD‐PPD)‐modified electrode. The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.
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DOI: 10.1002/elan.201000248
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Montserrat Cortina-Puig<affiliation><mods:affiliation>E-mail: mcortina12@gmail.com</mods:affiliation><wicri:noCountry code="no comma">E-mail: mcortina12@gmail.com</wicri:noCountry></affiliation><affiliation><mods:affiliation>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</mods:affiliation><wicri:noCountry code="subField">fax:+33 468662223</wicri:noCountry></affiliation><affiliation><mods:affiliation>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</mods:affiliation><wicri:noCountry code="subField">fax:+33 468662223</wicri:noCountry></affiliation>Le document en format XML
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The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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