Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity
Identifieur interne : 000554 ( Istex/Corpus ); précédent : 000553; suivant : 000555Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity
Auteurs : Montserrat Cortina-Puig ; Anastasia C. H. Scangas ; Zachary S. Marchese ; Silvana Andreescu ; Jean-Louis Marty ; Carole Calas-BlanchardSource :
- Electroanalysis [ 1040-0397 ] ; 2010-10.
English descriptors
Abstract
This paper describes the development of a xanthine oxidase/poly‐m‐phenylenediamine (XOD‐PPD)‐modified electrode. The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.
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DOI: 10.1002/elan.201000248
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The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Montserrat Cortina‐Puig</name><affiliations><json:string>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</json:string><json:string>E-mail: mcortina12@gmail.com</json:string></affiliations></json:item><json:item><name>Anastasia C. H. Scangas</name><affiliations><json:string>Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699‐5810, USA</json:string></affiliations></json:item><json:item><name>Zachary S. 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The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. 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The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. 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H.</givenNames><familyName>Scangas</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#ab"><personName><givenNames>Zachary S.</givenNames><familyName>Marchese</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#ab"><personName><givenNames>Silvana</givenNames><familyName>Andreescu</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#aa"><personName><givenNames>Jean‐Louis</givenNames><familyName>Marty</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#aa"><personName><givenNames>Carole</givenNames><familyName>Calas‐Blanchard</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aa" countryCode="FR" type="organization"><unparsedAffiliation>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</unparsedAffiliation></affiliation><affiliation xml:id="ab" countryCode="US" type="organization"><unparsedAffiliation>Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699‐5810, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">XOD‐based biosensor</keyword><keyword xml:id="kwd2">Superoxide radicals</keyword><keyword xml:id="kwd3">Antioxidant capacity</keyword><keyword xml:id="kwd4">PPD membrane</keyword><keyword xml:id="kwd5">Sol‐gel</keyword></keywordGroup><fundingInfo><fundingAgency>European Commission</fundingAgency></fundingInfo><fundingInfo><fundingAgency>“Nutra‐Snacks”</fundingAgency><fundingNumber>FOOD‐CT‐2005‐023044</fundingNumber></fundingInfo><fundingInfo><fundingAgency>US‐France International Research Experience for Students (IRES)</fundingAgency></fundingInfo><fundingInfo><fundingAgency>US National Science Foundation</fundingAgency><fundingNumber>NSF‐OISE‐0727861</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>This paper describes the development of a xanthine oxidase/poly‐<i>m</i>‐phenylenediamine (XOD‐PPD)‐modified electrode. The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity</title></titleInfo><name type="personal"><namePart type="given">Montserrat</namePart><namePart type="family">Cortina‐Puig</namePart><affiliation>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</affiliation><affiliation>E-mail: mcortina12@gmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Anastasia C. H.</namePart><namePart type="family">Scangas</namePart><affiliation>Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699‐5810, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zachary S.</namePart><namePart type="family">Marchese</namePart><affiliation>Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699‐5810, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Silvana</namePart><namePart type="family">Andreescu</namePart><affiliation>Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699‐5810, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean‐Louis</namePart><namePart type="family">Marty</namePart><affiliation>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Carole</namePart><namePart type="family">Calas‐Blanchard</namePart><affiliation>IMAGES EA 4218 Université de Perpignan Via Domitia, 52 avenue Paul Alduy, 66860 Perpignan Cedex, France tel: +33 468662253; fax:+33 468662223</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2010-10</dateIssued><dateCaptured encoding="w3cdtf">2010-04-09</dateCaptured><dateValid encoding="w3cdtf">2010-06-19</dateValid><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="references">25</extent></physicalDescription><abstract lang="en">This paper describes the development of a xanthine oxidase/poly‐m‐phenylenediamine (XOD‐PPD)‐modified electrode. The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.</abstract><note type="funding">European Commission</note><note type="funding">“Nutra‐Snacks” - No. FOOD‐CT‐2005‐023044; </note><note type="funding">US‐France International Research Experience for Students (IRES)</note><note type="funding">US National Science Foundation - No. NSF‐OISE‐0727861; </note><subject lang="en"><genre>keywords</genre><topic>XOD‐based biosensor</topic><topic>Superoxide radicals</topic><topic>Antioxidant capacity</topic><topic>PPD membrane</topic><topic>Sol‐gel</topic></subject><relatedItem type="host"><titleInfo><title>Electroanalysis</title></titleInfo><titleInfo type="abbreviated"><title>Electroanalysis</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Full Paper</topic></subject><identifier type="ISSN">1040-0397</identifier><identifier type="eISSN">1521-4109</identifier><identifier type="DOI">10.1002/(ISSN)1521-4109</identifier><identifier type="PublisherID">ELAN</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>22</number></detail><detail type="issue"><caption>no.</caption><number>20</number></detail><extent unit="pages"><start>2429</start><end>2433</end><total>5</total></extent></part></relatedItem><identifier type="istex">E1E10AE642AC7F1C7056F9B9E5BF9880EE467E81</identifier><identifier type="DOI">10.1002/elan.201000248</identifier><identifier type="ArticleID">ELAN201000248</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2010 WILEY‐VCH Verlag GmbH & Co. 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