Simultaneous Detection of Influenza Viruses A, B, and Swine Origin Influenza A Using Multiplex One-Step Real-Time RT-PCR Assay
Identifieur interne : 001014 ( Main/Exploration ); précédent : 001013; suivant : 001015Simultaneous Detection of Influenza Viruses A, B, and Swine Origin Influenza A Using Multiplex One-Step Real-Time RT-PCR Assay
Auteurs : S. H. R. Monavari [Iran] ; H. R. Mollaie [Iran] ; M. Fazlalipour [Iran]Source :
- Applied biochemistry and biotechnology [ 0273-2289 ] ; 2014.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Porcin.
English descriptors
- KwdEn :
Abstract
Every year, seasonal epidemics of influenza viruses are causing considerable morbidity and mortality worldwide. Also infrequent novel and rearranged strains of influenza viruses have caused quick, acute universal pandemics resulting in millions of mortalities. The usage of efficient and accurate detection is superior for infection control, effective treatment, and epidemiological supervision. Therefore, evaluation of useful real-time PCR molecular tests for the detection of pandemic viruses is important before the next wave of the pandemic. A novel quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers was used successfully for detection and monitoring of the influenza A, B, and swine influenza. The newly designed primers target highly conserved regions in influenza viruses. Our qRT-PCR assay is highly specific for detecting influenza A, B, and swine influenza viruses. The cutoff CT value was determined <38 for domestic human diagnostic test, under conditions of FDA emergency, and the reaction efficiency of the InfA, swInfA, and InfB assays were thereby estimated to be 97.9 % (R2=0.998), 98.3 % (R2=0.986), and 99.5 % (R2=0.995), respectively. Interestingly, based on our finding, there is no cross reactivity of detecting other viruses.
Affiliations:
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Mollaie</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Virology, Iran University of Medical Sciences</s1><s2>Tehran</s2><s3>IRN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Iran</country><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Fazlalipour, M" sort="Fazlalipour, M" uniqKey="Fazlalipour M" first="M." last="Fazlalipour">M. 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H. R." last="Monavari">S. H. R. Monavari</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology and Anti Microbial Resistance Research Center, Iran University of Medical Sciences, Hemmat Highway</s1><s2>Tehran</s2><s3>IRN</s3><sZ>1 aut.</sZ></inist:fA14><country>Iran</country><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Mollaie, H R" sort="Mollaie, H R" uniqKey="Mollaie H" first="H. R." last="Mollaie">H. R. Mollaie</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Virology, Iran University of Medical Sciences</s1><s2>Tehran</s2><s3>IRN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Iran</country><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Fazlalipour, M" sort="Fazlalipour, M" uniqKey="Fazlalipour M" first="M." last="Fazlalipour">M. Fazlalipour</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Virology, Iran University of Medical Sciences</s1><s2>Tehran</s2><s3>IRN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Iran</country><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Applied biochemistry and biotechnology</title><title level="j" type="abbreviated">Appl. biochem. biotechnol.</title><idno type="ISSN">0273-2289</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Applied biochemistry and biotechnology</title><title level="j" type="abbreviated">Appl. biochem. biotechnol.</title><idno type="ISSN">0273-2289</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term><term>Influenza A</term><term>Influenza B</term><term>Pig</term><term>Polymerase chain reaction</term><term>Real time</term><term>Reverse transcription polymerase chain reaction</term><term>Swine</term><term>Virus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Détection</term><term>Grippe A</term><term>Grippe B</term><term>Porc</term><term>Porcin</term><term>Virus</term><term>Temps réel</term><term>Réaction chaîne polymérase RT</term><term>Réaction chaîne polymérase</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Porcin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Every year, seasonal epidemics of influenza viruses are causing considerable morbidity and mortality worldwide. Also infrequent novel and rearranged strains of influenza viruses have caused quick, acute universal pandemics resulting in millions of mortalities. The usage of efficient and accurate detection is superior for infection control, effective treatment, and epidemiological supervision. Therefore, evaluation of useful real-time PCR molecular tests for the detection of pandemic viruses is important before the next wave of the pandemic. A novel quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers was used successfully for detection and monitoring of the influenza A, B, and swine influenza. The newly designed primers target highly conserved regions in influenza viruses. Our qRT-PCR assay is highly specific for detecting influenza A, B, and swine influenza viruses. The cutoff CT value was determined <38 for domestic human diagnostic test, under conditions of FDA emergency, and the reaction efficiency of the InfA, swInfA, and InfB assays were thereby estimated to be 97.9 % (R2=0.998), 98.3 % (R2=0.986), and 99.5 % (R2=0.995), respectively. Interestingly, based on our finding, there is no cross reactivity of detecting other viruses.</div></front></TEI><affiliations><list><country><li>Iran</li></country></list><tree><country name="Iran"><noRegion><name sortKey="Monavari, S H R" sort="Monavari, S H R" uniqKey="Monavari S" first="S. H. R." last="Monavari">S. H. R. Monavari</name></noRegion><name sortKey="Fazlalipour, M" sort="Fazlalipour, M" uniqKey="Fazlalipour M" first="M." last="Fazlalipour">M. Fazlalipour</name><name sortKey="Mollaie, H R" sort="Mollaie, H R" uniqKey="Mollaie H" first="H. R." last="Mollaie">H. R. Mollaie</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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