Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.
Identifieur interne : 002247 ( PubMed/Curation ); précédent : 002246; suivant : 002248Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.
Auteurs : Rong Chen [République populaire de Chine] ; Xuefang Luo ; Xin Di ; Ying Li ; Yuqing Sun ; Yuzhu HuSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Organothiophosphates.
- chemical , isolation & purification : Oligodeoxyribonucleotides, Organothiophosphates.
- chemical : Acetonitriles, Polyethylene Glycols, Urea.
- instrumentation : Electrophoresis, Capillary.
- methods : Electrophoresis, Capillary.
- Nucleic Acid Conformation.
Abstract
A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.
DOI: 10.1016/j.jchromb.2006.05.034
PubMed: 16809077
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<term>Nucleic Acid Conformation</term>
<term>Oligodeoxyribonucleotides (isolation & purification)</term>
<term>Organothiophosphates (chemistry)</term>
<term>Organothiophosphates (isolation & purification)</term>
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<term>Organothiophosphates ()</term>
<term>Organothiophosphates (isolement et purification)</term>
<term>Polyéthylène glycols</term>
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<front><div type="abstract" xml:lang="en">A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.</div>
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<Abstract><AbstractText>A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.</AbstractText>
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