Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.
Identifieur interne : 002247 ( PubMed/Corpus ); précédent : 002246; suivant : 002248Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.
Auteurs : Rong Chen ; Xuefang Luo ; Xin Di ; Ying Li ; Yuqing Sun ; Yuzhu HuSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2006.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Organothiophosphates.
- chemical , isolation & purification : Oligodeoxyribonucleotides, Organothiophosphates.
- chemical : Acetonitriles, Polyethylene Glycols, Urea.
- instrumentation : Electrophoresis, Capillary.
- methods : Electrophoresis, Capillary.
- Nucleic Acid Conformation.
Abstract
A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.
DOI: 10.1016/j.jchromb.2006.05.034
PubMed: 16809077
Links to Exploration step
pubmed:16809077Le document en format XML
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<author><name sortKey="Chen, Rong" sort="Chen, Rong" uniqKey="Chen R" first="Rong" last="Chen">Rong Chen</name>
<affiliation><nlm:affiliation>Department of Analytical Chemistry, China Pharmaceutical University, Box 41, 24 Tongjia Lane, Nanjing 210009, PR China.</nlm:affiliation>
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<author><name sortKey="Luo, Xuefang" sort="Luo, Xuefang" uniqKey="Luo X" first="Xuefang" last="Luo">Xuefang Luo</name>
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<author><name sortKey="Di, Xin" sort="Di, Xin" uniqKey="Di X" first="Xin" last="Di">Xin Di</name>
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<author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name>
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<author><name sortKey="Sun, Yuqing" sort="Sun, Yuqing" uniqKey="Sun Y" first="Yuqing" last="Sun">Yuqing Sun</name>
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<author><name sortKey="Hu, Yuzhu" sort="Hu, Yuzhu" uniqKey="Hu Y" first="Yuzhu" last="Hu">Yuzhu Hu</name>
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<author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name>
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<author><name sortKey="Sun, Yuqing" sort="Sun, Yuqing" uniqKey="Sun Y" first="Yuqing" last="Sun">Yuqing Sun</name>
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<term>Electrophoresis, Capillary (instrumentation)</term>
<term>Electrophoresis, Capillary (methods)</term>
<term>Nucleic Acid Conformation</term>
<term>Oligodeoxyribonucleotides (isolation & purification)</term>
<term>Organothiophosphates (chemistry)</term>
<term>Organothiophosphates (isolation & purification)</term>
<term>Polyethylene Glycols</term>
<term>Urea</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Organothiophosphates</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Oligodeoxyribonucleotides</term>
<term>Organothiophosphates</term>
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<term>Polyethylene Glycols</term>
<term>Urea</term>
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<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrophoresis, Capillary</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term>
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<front><div type="abstract" xml:lang="en">A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.</div>
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<ArticleTitle>Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis.</ArticleTitle>
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<Abstract><AbstractText>A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18-20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.</AbstractText>
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