MALDI analysis of oligonucleotides directly from montmorillonite.
Identifieur interne : 002248 ( PubMed/Curation ); précédent : 002247; suivant : 002249MALDI analysis of oligonucleotides directly from montmorillonite.
Auteurs : Dmitri V. Zagorevskii [États-Unis] ; Michael F. Aldersley ; James P. FerrisSource :
- Journal of the American Society for Mass Spectrometry [ 1044-0305 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Oligonucleotides.
- chemical , chemistry : Bentonite, Oligonucleotides.
- methods : Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Oligonucleotides synthesized on a montmorillonite catalyst were analyzed directly. By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by mass spectrometry even though their concentration was less than 10(-4)% of the total content of the RNA. This method is different from the (MALDI) analysis of the eluates from montmorillonite, which otherwise requires desalting. Placing reaction mixtures with a high concentration of buffers on homoionic, preferably Li-containing, montmorillonite does not require desalting.
DOI: 10.1016/j.jasms.2006.05.012
PubMed: 16809045
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By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by mass spectrometry even though their concentration was less than 10(-4)% of the total content of the RNA. This method is different from the (MALDI) analysis of the eluates from montmorillonite, which otherwise requires desalting. Placing reaction mixtures with a high concentration of buffers on homoionic, preferably Li-containing, montmorillonite does not require desalting.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16809045</PMID><DateCompleted><Year>2007</Year><Month>07</Month><Day>03</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1044-0305</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>9</Issue><PubDate><Year>2006</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of the American Society for Mass Spectrometry</Title><ISOAbbreviation>J. Am. Soc. 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