Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Identifieur interne : 001614 ( PubMed/Curation ); précédent : 001613; suivant : 001615Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Auteurs : Jasper Fuk-Woo Chan [République populaire de Chine] ; Garnet Kwan-Yue Choi [République populaire de Chine] ; Alan Ka-Lun Tsang [République populaire de Chine] ; Kah-Meng Tee [République populaire de Chine] ; Ho-Yin Lam [République populaire de Chine] ; Cyril Chik-Yan Yip [République populaire de Chine] ; Kelvin Kai-Wang To [République populaire de Chine] ; Vincent Chi-Chung Cheng [République populaire de Chine] ; Man-Lung Yeung [République populaire de Chine] ; Susanna Kar-Pui Lau [République populaire de Chine] ; Patrick Chiu-Yat Woo [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; Bone Siu-Fai Tang [République populaire de Chine] ; Kwok-Yung Yuen [Hong Kong]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Jeune adulte, Mâle, Nourrisson, Oligonucléotides (génétique), RT-PCR (), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Sondes oligonucléotidiques (génétique), Sujet âgé, Sujet âgé de 80 ans ou plus.
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus, Oligonucléotides, Sondes oligonucléotidiques.
- isolement et purification : Coronavirus.
- virologie : Infections à coronavirus.
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Coronavirus, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Nourrisson, RT-PCR, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Sujet âgé, Sujet âgé de 80 ans ou plus.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Female, Humans, Infant, Male, Middle Aged, Oligonucleotide Probes (genetics), Oligonucleotides (genetics), Real-Time Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Young Adult.
- MESH :
- chemical , genetics : Oligonucleotide Probes, Oligonucleotides.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
DOI: 10.1128/JCM.01224-15
PubMed: 26019210
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pubmed:26019210Le document en format XML
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<author><name sortKey="Yip, Cyril Chik Yan" sort="Yip, Cyril Chik Yan" uniqKey="Yip C" first="Cyril Chik-Yan" last="Yip">Cyril Chik-Yan Yip</name>
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<author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name>
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<author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name>
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<author><name sortKey="Tee, Kah Meng" sort="Tee, Kah Meng" uniqKey="Tee K" first="Kah-Meng" last="Tee">Kah-Meng Tee</name>
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<author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name>
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<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
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<author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name>
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<author><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man-Lung" last="Yeung">Man-Lung Yeung</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
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<author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name>
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<author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
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<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
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<wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea>
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<author><name sortKey="Tang, Bone Siu Fai" sort="Tang, Bone Siu Fai" uniqKey="Tang B" first="Bone Siu-Fai" last="Tang">Bone Siu-Fai Tang</name>
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<wicri:regionArea>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region</wicri:regionArea>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<front><div type="abstract" xml:lang="en">Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses. </div>
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<DateCompleted><Year>2016</Year>
<Month>05</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-660X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>53</Volume>
<Issue>8</Issue>
<PubDate><Year>2015</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Journal of clinical microbiology</Title>
<ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.</ArticleTitle>
<Pagination><MedlinePgn>2722-6</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1128/JCM.01224-15</ELocationID>
<Abstract><AbstractText>Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses. </AbstractText>
<CopyrightInformation>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Jasper Fuk-Woo</ForeName>
<Initials>JF</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Choi</LastName>
<ForeName>Garnet Kwan-Yue</ForeName>
<Initials>GK</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tsang</LastName>
<ForeName>Alan Ka-Lun</ForeName>
<Initials>AK</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tee</LastName>
<ForeName>Kah-Meng</ForeName>
<Initials>KM</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lam</LastName>
<ForeName>Ho-Yin</ForeName>
<Initials>HY</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yip</LastName>
<ForeName>Cyril Chik-Yan</ForeName>
<Initials>CC</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
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<ForeName>Kelvin Kai-Wang</ForeName>
<Initials>KK</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
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<ForeName>Vincent Chi-Chung</ForeName>
<Initials>VC</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yeung</LastName>
<ForeName>Man-Lung</ForeName>
<Initials>ML</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lau</LastName>
<ForeName>Susanna Kar-Pui</ForeName>
<Initials>SK</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Woo</LastName>
<ForeName>Patrick Chiu-Yat</ForeName>
<Initials>PC</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Kwok-Hung</ForeName>
<Initials>KH</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tang</LastName>
<ForeName>Bone Siu-Fai</ForeName>
<Initials>BS</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yuen</LastName>
<ForeName>Kwok-Yung</ForeName>
<Initials>KY</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
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<PublicationType UI="D023362">Evaluation Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
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<Month>05</Month>
<Day>27</Day>
</ArticleDate>
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