Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Identifieur interne : 001614 ( PubMed/Corpus ); précédent : 001613; suivant : 001615Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Auteurs : Jasper Fuk-Woo Chan ; Garnet Kwan-Yue Choi ; Alan Ka-Lun Tsang ; Kah-Meng Tee ; Ho-Yin Lam ; Cyril Chik-Yan Yip ; Kelvin Kai-Wang To ; Vincent Chi-Chung Cheng ; Man-Lung Yeung ; Susanna Kar-Pui Lau ; Patrick Chiu-Yat Woo ; Kwok-Hung Chan ; Bone Siu-Fai Tang ; Kwok-Yung YuenSource :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Female, Humans, Infant, Male, Middle Aged, Oligonucleotide Probes (genetics), Oligonucleotides (genetics), Real-Time Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Young Adult.
- MESH :
- chemical , genetics : Oligonucleotide Probes, Oligonucleotides.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
DOI: 10.1128/JCM.01224-15
PubMed: 26019210
Links to Exploration step
pubmed:26019210Le document en format XML
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Yue" uniqKey="Choi G" first="Garnet Kwan-Yue" last="Choi">Garnet Kwan-Yue Choi</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tee, Kah Meng" sort="Tee, Kah Meng" uniqKey="Tee K" first="Kah-Meng" last="Tee">Kah-Meng Tee</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lam, Ho Yin" sort="Lam, Ho Yin" uniqKey="Lam H" first="Ho-Yin" last="Lam">Ho-Yin Lam</name><affiliation><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yip, Cyril Chik Yan" sort="Yip, Cyril Chik Yan" uniqKey="Yip C" first="Cyril Chik-Yan" last="Yip">Cyril Chik-Yan Yip</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man-Lung" last="Yeung">Man-Lung Yeung</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tang, Bone Siu Fai" sort="Tang, Bone Siu Fai" uniqKey="Tang B" first="Bone Siu-Fai" last="Tang">Bone Siu-Fai Tang</name><affiliation><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26019210</idno><idno type="pmid">26019210</idno><idno type="doi">10.1128/JCM.01224-15</idno><idno type="wicri:Area/PubMed/Corpus">001614</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001614</idno></publicationStmt><sourceDesc><biblStruct><analytic><title 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Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yip, Cyril Chik Yan" sort="Yip, Cyril Chik Yan" uniqKey="Yip C" first="Cyril Chik-Yan" last="Yip">Cyril Chik-Yan Yip</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man-Lung" last="Yeung">Man-Lung Yeung</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tang, Bone Siu Fai" sort="Tang, Bone Siu Fai" uniqKey="Tang B" first="Bone Siu-Fai" last="Tang">Bone Siu-Fai Tang</name><affiliation><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Child</term><term>Child, Preschool</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Oligonucleotide Probes (genetics)</term><term>Oligonucleotides (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oligonucleotide Probes</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26019210</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-660X</ISSN><JournalIssue CitedMedium="Internet"><Volume>53</Volume><Issue>8</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.</ArticleTitle><Pagination><MedlinePgn>2722-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JCM.01224-15</ELocationID><Abstract><AbstractText>Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses. </AbstractText><CopyrightInformation>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Jasper Fuk-Woo</ForeName><Initials>JF</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Garnet Kwan-Yue</ForeName><Initials>GK</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsang</LastName><ForeName>Alan Ka-Lun</ForeName><Initials>AK</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tee</LastName><ForeName>Kah-Meng</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lam</LastName><ForeName>Ho-Yin</ForeName><Initials>HY</Initials><AffiliationInfo><Affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yip</LastName><ForeName>Cyril Chik-Yan</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>To</LastName><ForeName>Kelvin Kai-Wang</ForeName><Initials>KK</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Vincent Chi-Chung</ForeName><Initials>VC</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yeung</LastName><ForeName>Man-Lung</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lau</LastName><ForeName>Susanna Kar-Pui</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Woo</LastName><ForeName>Patrick Chiu-Yat</ForeName><Initials>PC</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Kwok-Hung</ForeName><Initials>KH</Initials><AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Bone Siu-Fai</ForeName><Initials>BS</Initials><AffiliationInfo><Affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuen</LastName><ForeName>Kwok-Yung</ForeName><Initials>KY</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType 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