MersV1, PubMed, Corpus, bibRecord, 002A87

[General approach to the engineering of synthetic DNA].

Identifieur interne : 002A87 ( PubMed/Corpus ); précédent : 002A86; suivant : 002A88

[General approach to the engineering of synthetic DNA].

Auteurs : O G Chakhmakhcheva ; A A Buriakova ; O V Mirskikh ; S V Reverdatto ; V A Efimov

Source :

Bioorganicheskaia khimiia [ 0132-3423 ] ; 1985.

RBID : pubmed:3004509

English descriptors

KwdEn :
- Bacteriorhodopsins (genetics), Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA (chemical synthesis), DNA Restriction Enzymes, DNA, Bacterial (chemical synthesis), Escherichia coli (genetics), Genes, Synthetic, Genetic Engineering (methods), Genetic Vectors, Plasmids, Polynucleotides (chemical synthesis).
MESH :
- chemical , chemical synthesis : DNA, DNA, Bacterial, Polynucleotides.
- chemical , genetics : Bacteriorhodopsins.
- genetics : Escherichia coli.
- methods : Genetic Engineering.
- Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA Restriction Enzymes, Genes, Synthetic, Genetic Vectors, Plasmids.

Abstract

A useful and efficient approach to the synthesis of DNA duplexes of practically unlimited length has been developed. The proposed methodology is based on the use of temporary restriction sites for subcloning and assembling the segments of the desired DNA. It allows the utilization of chemically synthesized oligonucleotides of various length (from 10- to 100-mers) for the duplex construction. The application of this approach to the synthesis of a gene for the functionally active bacteriorhodopsin fragment is described.

PubMed: 3004509

Links to Exploration step

pubmed:3004509

Le document en format XML

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<author><name sortKey="Chakhmakhcheva, O G" sort="Chakhmakhcheva, O G" uniqKey="Chakhmakhcheva O" first="O G" last="Chakhmakhcheva">O G Chakhmakhcheva</name>
</author>
<author><name sortKey="Buriakova, A A" sort="Buriakova, A A" uniqKey="Buriakova A" first="A A" last="Buriakova">A A Buriakova</name>
</author>
<author><name sortKey="Mirskikh, O V" sort="Mirskikh, O V" uniqKey="Mirskikh O" first="O V" last="Mirskikh">O V Mirskikh</name>
</author>
<author><name sortKey="Reverdatto, S V" sort="Reverdatto, S V" uniqKey="Reverdatto S" first="S V" last="Reverdatto">S V Reverdatto</name>
</author>
<author><name sortKey="Efimov, V A" sort="Efimov, V A" uniqKey="Efimov V" first="V A" last="Efimov">V A Efimov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1985">1985</date>
<idno type="RBID">pubmed:3004509</idno>
<idno type="pmid">3004509</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[General approach to the engineering of synthetic DNA].</title>
<author><name sortKey="Chakhmakhcheva, O G" sort="Chakhmakhcheva, O G" uniqKey="Chakhmakhcheva O" first="O G" last="Chakhmakhcheva">O G Chakhmakhcheva</name>
</author>
<author><name sortKey="Buriakova, A A" sort="Buriakova, A A" uniqKey="Buriakova A" first="A A" last="Buriakova">A A Buriakova</name>
</author>
<author><name sortKey="Mirskikh, O V" sort="Mirskikh, O V" uniqKey="Mirskikh O" first="O V" last="Mirskikh">O V Mirskikh</name>
</author>
<author><name sortKey="Reverdatto, S V" sort="Reverdatto, S V" uniqKey="Reverdatto S" first="S V" last="Reverdatto">S V Reverdatto</name>
</author>
<author><name sortKey="Efimov, V A" sort="Efimov, V A" uniqKey="Efimov V" first="V A" last="Efimov">V A Efimov</name>
</author>
</analytic>
<series><title level="j">Bioorganicheskaia khimiia</title>
<idno type="ISSN">0132-3423</idno>
<imprint><date when="1985" type="published">1985</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriorhodopsins (genetics)</term>
<term>Base Sequence</term>
<term>Chromosome Mapping</term>
<term>Cloning, Molecular</term>
<term>DNA (chemical synthesis)</term>
<term>DNA Restriction Enzymes</term>
<term>DNA, Bacterial (chemical synthesis)</term>
<term>Escherichia coli (genetics)</term>
<term>Genes, Synthetic</term>
<term>Genetic Engineering (methods)</term>
<term>Genetic Vectors</term>
<term>Plasmids</term>
<term>Polynucleotides (chemical synthesis)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>DNA</term>
<term>DNA, Bacterial</term>
<term>Polynucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacteriorhodopsins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Chromosome Mapping</term>
<term>Cloning, Molecular</term>
<term>DNA Restriction Enzymes</term>
<term>Genes, Synthetic</term>
<term>Genetic Vectors</term>
<term>Plasmids</term>
</keywords>
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</teiHeader>
<front><div type="abstract" xml:lang="en">A useful and efficient approach to the synthesis of DNA duplexes of practically unlimited length has been developed. The proposed methodology is based on the use of temporary restriction sites for subcloning and assembling the segments of the desired DNA. It allows the utilization of chemically synthesized oligonucleotides of various length (from 10- to 100-mers) for the duplex construction. The application of this approach to the synthesis of a gene for the functionally active bacteriorhodopsin fragment is described.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3004509</PMID>
<DateCompleted><Year>1986</Year>
<Month>03</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0132-3423</ISSN>
<JournalIssue CitedMedium="Print"><Volume>11</Volume>
<Issue>11</Issue>
<PubDate><Year>1985</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Bioorganicheskaia khimiia</Title>
<ISOAbbreviation>Bioorg. Khim.</ISOAbbreviation>
</Journal>
<ArticleTitle>[General approach to the engineering of synthetic DNA].</ArticleTitle>
<Pagination><MedlinePgn>1533-46</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A useful and efficient approach to the synthesis of DNA duplexes of practically unlimited length has been developed. The proposed methodology is based on the use of temporary restriction sites for subcloning and assembling the segments of the desired DNA. It allows the utilization of chemically synthesized oligonucleotides of various length (from 10- to 100-mers) for the duplex construction. The application of this approach to the synthesis of a gene for the functionally active bacteriorhodopsin fragment is described.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chakhmakhcheva</LastName>
<ForeName>O G</ForeName>
<Initials>OG</Initials>
</Author>
<Author ValidYN="Y"><LastName>Buriakova</LastName>
<ForeName>A A</ForeName>
<Initials>AA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Mirskikh</LastName>
<ForeName>O V</ForeName>
<Initials>OV</Initials>
</Author>
<Author ValidYN="Y"><LastName>Reverdatto</LastName>
<ForeName>S V</ForeName>
<Initials>SV</Initials>
</Author>
<Author ValidYN="Y"><LastName>Efimov</LastName>
<ForeName>V A</ForeName>
<Initials>VA</Initials>
</Author>
</AuthorList>
<Language>rus</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<VernacularTitle>Obshchiĭ podkhod k konstruirovaniiu sinteticheskikh DNK.</VernacularTitle>
</Article>
<MedlineJournalInfo><Country>Russia (Federation)</Country>
<MedlineTA>Bioorg Khim</MedlineTA>
<NlmUniqueID>7804941</NlmUniqueID>
<ISSNLinking>0132-3423</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011119">Polynucleotides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>53026-44-1</RegistryNumber>
<NameOfSubstance UI="D001436">Bacteriorhodopsins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-49-2</RegistryNumber>
<NameOfSubstance UI="D004247">DNA</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.21.-</RegistryNumber>
<NameOfSubstance UI="D004262">DNA Restriction Enzymes</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001436" MajorTopicYN="N">Bacteriorhodopsins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName>
<QualifierName UI="Q000138" MajorTopicYN="Y">chemical synthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004262" MajorTopicYN="N">DNA Restriction Enzymes</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName>
<QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005813" MajorTopicYN="Y">Genes, Synthetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011119" MajorTopicYN="N">Polynucleotides</DescriptorName>
<QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1985</Year>
<Month>11</Month>
<Day>1</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1985</Year>
<Month>11</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
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<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">3004509</ArticleId>
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</pubmed>
</record>

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[General approach to the engineering of synthetic DNA].

[General approach to the engineering of synthetic DNA].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki