MersV1, PubMed, Corpus, bibRecord, 002A86

Curved DNA: design, synthesis, and circularization.

Identifieur interne : 002A86 ( PubMed/Corpus ); précédent : 002A85; suivant : 002A87

Curved DNA: design, synthesis, and circularization.

Auteurs : L. Ulanovsky ; M. Bodner ; E N Trifonov ; M. Choder

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1986.

RBID : pubmed:3456570

English descriptors

KwdEn :
- DNA (chemical synthesis), DNA, Circular (chemical synthesis), Electrophoresis, Polyacrylamide Gel, Molecular Weight, Nucleic Acid Conformation, Oligodeoxyribonucleotides (chemical synthesis).
MESH :
- chemical , chemical synthesis : DNA, DNA, Circular, Oligodeoxyribonucleotides.
- Electrophoresis, Polyacrylamide Gel, Molecular Weight, Nucleic Acid Conformation.

Abstract

Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide gel electrophoresis of the ligation products showed two distinct families of spots: (i) noncircular oligo(precursor)s of 21 to 231 bp (1- to 11-mers) and (ii) four circles from 105 to 168 bp (eluted and analyzed by denaturing gel electrophoresis). The noncircular oligomers exhibited anomalously slow migration, as if they were as much as three times longer than they actually are. The amount of circular products peaked sharply at approximately equal to 126 bp, near which size the circles have been estimated to be nonconstrained both torsionally and in terms of bending. The nonconstrained circularization provides a technique for the direct measurement of the inherent curvature of DNA in solution. From the size of the circles, an estimate of 8.7 degrees is obtained for the absolute value of the AA X TT wedge angle (roll and tilt combined).

DOI: 10.1073/pnas.83.4.862
PubMed: 3456570

Links to Exploration step

pubmed:3456570

Le document en format XML

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<author><name sortKey="Ulanovsky, L" sort="Ulanovsky, L" uniqKey="Ulanovsky L" first="L" last="Ulanovsky">L. Ulanovsky</name>
</author>
<author><name sortKey="Bodner, M" sort="Bodner, M" uniqKey="Bodner M" first="M" last="Bodner">M. Bodner</name>
</author>
<author><name sortKey="Trifonov, E N" sort="Trifonov, E N" uniqKey="Trifonov E" first="E N" last="Trifonov">E N Trifonov</name>
</author>
<author><name sortKey="Choder, M" sort="Choder, M" uniqKey="Choder M" first="M" last="Choder">M. Choder</name>
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<date when="1986">1986</date>
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<author><name sortKey="Ulanovsky, L" sort="Ulanovsky, L" uniqKey="Ulanovsky L" first="L" last="Ulanovsky">L. Ulanovsky</name>
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<author><name sortKey="Bodner, M" sort="Bodner, M" uniqKey="Bodner M" first="M" last="Bodner">M. Bodner</name>
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<author><name sortKey="Trifonov, E N" sort="Trifonov, E N" uniqKey="Trifonov E" first="E N" last="Trifonov">E N Trifonov</name>
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<author><name sortKey="Choder, M" sort="Choder, M" uniqKey="Choder M" first="M" last="Choder">M. Choder</name>
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<imprint><date when="1986" type="published">1986</date>
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<term>Molecular Weight</term>
<term>Nucleic Acid Conformation</term>
<term>Oligodeoxyribonucleotides (chemical synthesis)</term>
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<term>Oligodeoxyribonucleotides</term>
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<front><div type="abstract" xml:lang="en">Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide gel electrophoresis of the ligation products showed two distinct families of spots: (i) noncircular oligo(precursor)s of 21 to 231 bp (1- to 11-mers) and (ii) four circles from 105 to 168 bp (eluted and analyzed by denaturing gel electrophoresis). The noncircular oligomers exhibited anomalously slow migration, as if they were as much as three times longer than they actually are. The amount of circular products peaked sharply at approximately equal to 126 bp, near which size the circles have been estimated to be nonconstrained both torsionally and in terms of bending. The nonconstrained circularization provides a technique for the direct measurement of the inherent curvature of DNA in solution. From the size of the circles, an estimate of 8.7 degrees is obtained for the absolute value of the AA X TT wedge angle (roll and tilt combined).</div>
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<DateRevised><Year>2019</Year>
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<Title>Proceedings of the National Academy of Sciences of the United States of America</Title>
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<ArticleTitle>Curved DNA: design, synthesis, and circularization.</ArticleTitle>
<Pagination><MedlinePgn>862-6</MedlinePgn>
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<Abstract><AbstractText>Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide gel electrophoresis of the ligation products showed two distinct families of spots: (i) noncircular oligo(precursor)s of 21 to 231 bp (1- to 11-mers) and (ii) four circles from 105 to 168 bp (eluted and analyzed by denaturing gel electrophoresis). The noncircular oligomers exhibited anomalously slow migration, as if they were as much as three times longer than they actually are. The amount of circular products peaked sharply at approximately equal to 126 bp, near which size the circles have been estimated to be nonconstrained both torsionally and in terms of bending. The nonconstrained circularization provides a technique for the direct measurement of the inherent curvature of DNA in solution. From the size of the circles, an estimate of 8.7 degrees is obtained for the absolute value of the AA X TT wedge angle (roll and tilt combined).</AbstractText>
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Curved DNA: design, synthesis, and circularization.

Curved DNA: design, synthesis, and circularization.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki