Performance of FilmArray RP2 Multiplex Panel for identification of MERS CoV
Identifieur interne : 000614 ( Pmc/Corpus ); précédent : 000613; suivant : 000615Performance of FilmArray RP2 Multiplex Panel for identification of MERS CoV
Auteurs : T. Al Salmi ; A. Algothmi ; A. Alshehri ; S. AljohaniSource :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2019.
Url:
DOI: 10.1016/j.jiph.2018.10.019
PubMed: NONE
PubMed Central: 7129424
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Performance of FilmArray RP2 Multiplex Panel for identification of MERS CoV</title><author><name sortKey="Al Salmi, T" sort="Al Salmi, T" uniqKey="Al Salmi T" first="T." last="Al Salmi">T. Al Salmi</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation></author><author><name sortKey="Algothmi, A" sort="Algothmi, A" uniqKey="Algothmi A" first="A." last="Algothmi">A. Algothmi</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation></author><author><name sortKey="Alshehri, A" sort="Alshehri, A" uniqKey="Alshehri A" first="A." last="Alshehri">A. Alshehri</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation></author><author><name sortKey="Aljohani, S" sort="Aljohani, S" uniqKey="Aljohani S" first="S." last="Aljohani">S. Aljohani</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation><affiliation><nlm:aff id="aff0010">King Saud Bin Abdulaziz University for Health Sciences</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7129424</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129424</idno><idno type="RBID">PMC:7129424</idno><idno type="doi">10.1016/j.jiph.2018.10.019</idno><idno type="pmid">NONE</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000614</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000614</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Performance of FilmArray RP2 Multiplex Panel for identification of MERS CoV</title><author><name sortKey="Al Salmi, T" sort="Al Salmi, T" uniqKey="Al Salmi T" first="T." last="Al Salmi">T. Al Salmi</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation></author><author><name sortKey="Algothmi, A" sort="Algothmi, A" uniqKey="Algothmi A" first="A." last="Algothmi">A. Algothmi</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation></author><author><name sortKey="Alshehri, A" sort="Alshehri, A" uniqKey="Alshehri A" first="A." last="Alshehri">A. Alshehri</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation></author><author><name sortKey="Aljohani, S" sort="Aljohani, S" uniqKey="Aljohani S" first="S." last="Aljohani">S. Aljohani</name><affiliation><nlm:aff id="aff0005">King Abdulaziz Medical City</nlm:aff></affiliation><affiliation><nlm:aff id="aff0010">King Saud Bin Abdulaziz University for Health Sciences</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader></TEI><pmc article-type="abstract"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Infect Public Health</journal-id><journal-id journal-id-type="iso-abbrev">J Infect Public Health</journal-id><journal-title-group><journal-title>Journal of Infection and Public Health</journal-title></journal-title-group><issn pub-type="ppub">1876-0341</issn><issn pub-type="epub">1876-035X</issn><publisher><publisher-name>Published by Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">7129424</article-id><article-id pub-id-type="publisher-id">S1876-0341(18)30189-8</article-id><article-id pub-id-type="doi">10.1016/j.jiph.2018.10.019</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Performance of FilmArray RP2 Multiplex Panel for identification of MERS CoV</article-title></title-group><contrib-group><contrib contrib-type="author" id="aut0005"><name><surname>Al salmi</surname><given-names>T.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="cor0005" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="aut0010"><name><surname>Algothmi</surname><given-names>A.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0015"><name><surname>Alshehri</surname><given-names>A.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref></contrib><contrib contrib-type="author" id="aut0020"><name><surname>Aljohani</surname><given-names>S.</given-names></name><xref rid="aff0005" ref-type="aff">1</xref><xref rid="aff0010" ref-type="aff">2</xref></contrib></contrib-group><aff id="aff0005"><label>1</label>King Abdulaziz Medical City</aff><aff id="aff0010"><label>2</label>King Saud Bin Abdulaziz University for Health Sciences</aff><author-notes><corresp id="cor0005"><label>⁎</label>Corresponding author.</corresp></author-notes><pub-date pub-type="pmc-release"><day>8</day><month>1</month><year>2019</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub" iso-8601-date="2019-02-01"><season>January-February</season><year>2019</year></pub-date><pub-date pub-type="epub"><day>8</day><month>1</month><year>2019</year></pub-date><volume>12</volume><issue>1</issue><fpage>109</fpage><lpage>109</lpage><permissions><copyright-statement>Copyright © 2018 Published by Elsevier Ltd.</copyright-statement><copyright-year>2018</copyright-year><copyright-holder></copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions></article-meta></front><body><p id="par0005"><bold>Background and objective:</bold> The FilmArray Respiratory Panel 2 (RP2) is a multiplex in vitro diagnostic test for the simultaneous and rapid (∼45-min) detection of 22 pathogens directly from nasopharyngeal swab (NPS) samples. It contains updated (and in some instances redesigned) assays that improve upon the FilmArray Respiratory Panel (RP; version 1.7), with a faster run time. The organisms identified are adenovirus, coronavirus 229E, coronavirus HKU1, coronavirus NL63, coronavirus OC43, human metapneumovirus, human rhinovirus/enterovirus, influenza virus A, influenza virus A H1, influenza virus A H1-2009, influenza virus A H3, influenza virus B, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, parainfluenza virus 4, respiratory syncytial virus, Bordetella pertussis, Chlamydia pneumoniae, and Mycoplasma pneumoniae Two new targets are included in the FilmArray RP2: Middle East respiratory syndrome coronavirus and Bordetella parapertussis.</p><p id="par0010">In this study, we are evaluating the performance and accuracy of BioFire FilmArray in comparison to our gold standard method MERS-Cov using Roche MagnPure 96 and Light Cycler 480.</p><p id="par0015"><bold>Methods:</bold> 25 clinical samples were tested parallel using both Roche Light Cycler then repeated by using Respiratory Multiplex Panel 2 and performed on BioFire FilmArray.</p><p id="par0020">All tested samples were respiratory such as Sputum, Tracheal aspiration, Endotracheal, Nasopharyngeal aspiration and Nasopharyngeal Swabs.</p><p id="par0025"><bold>Result:</bold> 25 Patient samples were tested for MERS CoV. Overall positive percent agreement between systems was 99% and the negative percent agreement was 95%.</p><p id="par0030"><bold>Conclusion:</bold> In comparison with Roche Light Cycler, there were no false positive results observe from FilmArray system, which indicate that the FilmArray system design and effective in preventing carryover and cross contamination. However, we found that FilmArray system has more reliability and accuracy than Roche Light Cycler System. Moreover, easy to perform the test and the entire process takes about one hour, which dramatically changed the Turn-Around-Time to report the result.</p></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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