DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice
Identifieur interne : 000893 ( Pmc/Checkpoint ); précédent : 000892; suivant : 000894DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice
Auteurs : Hang Chi [République populaire de Chine] ; Xuexing Zheng [République populaire de Chine] ; Xiwen Wang [République populaire de Chine] ; Chong Wang [République populaire de Chine] ; Hualei Wang [République populaire de Chine] ; Weiwei Gai [République populaire de Chine] ; Stanley Perlman [États-Unis] ; Songtao Yang [République populaire de Chine] ; Jincun Zhao [République populaire de Chine] ; Xianzhu Xia [République populaire de Chine]Source :
- Vaccine [ 0264-410X ] ; 2017.
Abstract
DNA vaccine encoding MERS-CoV S1 gene induced humoral and cellular immune responses. High titers of neutralizing antibodies were generated without adjuvant. Virus loads in lungs significantly decreased in vaccinated and serum received mice.
Url:
DOI: 10.1016/j.vaccine.2017.02.063
PubMed: 28314561
PubMed Central: 5411280
Affiliations:
- République populaire de Chine, États-Unis
- Dongbei, Guangdong, Iowa, Jilin
- Changchun, Iowa City, Jiangmen
- Université de l'Iowa
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type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name><affiliation wicri:level="3"><nlm:aff id="af005">Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="af010">School of Public Health, Shandong University, Jinan, China</nlm:aff><country 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sort="Wang, Chong" uniqKey="Wang C" first="Chong" last="Wang">Chong Wang</name><affiliation wicri:level="3"><nlm:aff id="af005">Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><affiliation wicri:level="3"><nlm:aff id="af005">Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="af015">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Gai, Weiwei" sort="Gai, Weiwei" uniqKey="Gai W" first="Weiwei" last="Gai">Weiwei Gai</name><affiliation wicri:level="3"><nlm:aff id="af005">Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation wicri:level="4"><nlm:aff id="af020">Department of Microbiology, University of Iowa, Iowa City, IA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Iowa, Iowa City, IA</wicri:regionArea><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><affiliation wicri:level="3"><nlm:aff id="af005">Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="af015">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><affiliation wicri:level="3"><nlm:aff id="af025">State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation wicri:level="3"><nlm:aff id="af005">Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="af015">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label><p id="p0005">DNA vaccine encoding MERS-CoV S1 gene induced humoral and cellular immune responses.</p></list-item><list-item id="o0010"><label>•</label><p id="p0010">High titers of neutralizing antibodies were generated without adjuvant.</p></list-item><list-item id="o0015"><label>•</label><p id="p0015">Virus loads in lungs significantly decreased in vaccinated and serum received mice.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id><journal-id journal-id-type="iso-abbrev">Vaccine</journal-id><journal-title-group><journal-title>Vaccine</journal-title></journal-title-group><issn pub-type="ppub">0264-410X</issn><issn pub-type="epub">1873-2518</issn><publisher><publisher-name>Elsevier Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28314561</article-id><article-id pub-id-type="pmc">5411280</article-id><article-id pub-id-type="publisher-id">S0264-410X(17)30288-8</article-id><article-id pub-id-type="doi">10.1016/j.vaccine.2017.02.063</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice</article-title></title-group><contrib-group><contrib contrib-type="author" id="au005"><name><surname>Chi</surname><given-names>Hang</given-names></name><xref rid="af005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au010"><name><surname>Zheng</surname><given-names>Xuexing</given-names></name><xref rid="af005" ref-type="aff">a</xref><xref rid="af010" ref-type="aff">b</xref></contrib><contrib contrib-type="author" id="au015"><name><surname>Wang</surname><given-names>Xiwen</given-names></name><xref rid="af005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au020"><name><surname>Wang</surname><given-names>Chong</given-names></name><xref rid="af005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au025"><name><surname>Wang</surname><given-names>Hualei</given-names></name><xref rid="af005" ref-type="aff">a</xref><xref rid="af015" ref-type="aff">c</xref></contrib><contrib contrib-type="author" id="au030"><name><surname>Gai</surname><given-names>Weiwei</given-names></name><xref rid="af005" ref-type="aff">a</xref></contrib><contrib contrib-type="author" id="au035"><name><surname>Perlman</surname><given-names>Stanley</given-names></name><xref rid="af020" ref-type="aff">d</xref></contrib><contrib contrib-type="author" id="au040"><name><surname>Yang</surname><given-names>Songtao</given-names></name><email>yst62041@163.com</email><xref rid="af005" ref-type="aff">a</xref><xref rid="af015" ref-type="aff">c</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="au045"><name><surname>Zhao</surname><given-names>Jincun</given-names></name><email>zhaojincun@gird.cn</email><xref rid="af025" ref-type="aff">e</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib><contrib contrib-type="author" id="au050"><name><surname>Xia</surname><given-names>Xianzhu</given-names></name><email>xiaxzh@cae.cn</email><xref rid="af005" ref-type="aff">a</xref><xref rid="af015" ref-type="aff">c</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="af005"><label>a</label>Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China</aff><aff id="af010"><label>b</label>School of Public Health, Shandong University, Jinan, China</aff><aff id="af015"><label>c</label>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</aff><aff id="af020"><label>d</label>Department of Microbiology, University of Iowa, Iowa City, IA, USA</aff><aff id="af025"><label>e</label>State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding authors at: Department of Virology, Institute of Military Veterinary, Academy of Military Medical Sciences, 666 Liuying West Road, Changchun, Jilin 130012, China (S. Yang and X. Xia). State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China (J. Zhao). <email>yst62041@163.com</email><email>zhaojincun@gird.cn</email><email>xiaxzh@cae.cn</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>14</day><month>3</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>11</day><month>4</month><year>2017</year></pub-date><pub-date pub-type="epub"><day>14</day><month>3</month><year>2017</year></pub-date><volume>35</volume><issue>16</issue><fpage>2069</fpage><lpage>2075</lpage><history><date date-type="received"><day>10</day><month>6</month><year>2016</year></date><date date-type="rev-recd"><day>13</day><month>2</month><year>2017</year></date><date date-type="accepted"><day>28</day><month>2</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>Elsevier Ltd</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract abstract-type="author-highlights" id="ab005"><title>Highlights</title><p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label><p id="p0005">DNA vaccine encoding MERS-CoV S1 gene induced humoral and cellular immune responses.</p></list-item><list-item id="o0010"><label>•</label><p id="p0010">High titers of neutralizing antibodies were generated without adjuvant.</p></list-item><list-item id="o0015"><label>•</label><p id="p0015">Virus loads in lungs significantly decreased in vaccinated and serum received mice.</p></list-item></list></p></abstract><abstract id="ab010"><p>The Middle East respiratory syndrome coronavirus (MERS-CoV), is an emerging pathogen that continues to cause outbreaks in the Arabian peninsula and in travelers from this region, raising the concern that a global pandemic could occur. Here, we show that a DNA vaccine encoding the first 725 amino acids (S1) of MERS-CoV spike (S) protein induces antigen-specific humoral and cellular immune responses in mice. With three immunizations, high titers of neutralizing antibodies (up to 1: 10<sup>4</sup>) were generated without adjuvant. DNA vaccination with the MERS-CoV S1 gene markedly increased the frequencies of antigen-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cells secreting IFN-γ and other cytokines. Both pcDNA3.1-S1 DNA vaccine immunization and passive transfer of immune serum from pcDNA3.1-S1 vaccinated mice protected Ad5-hDPP4-transduced mice from MERS-CoV challenge. These results demonstrate that a DNA vaccine encoding MERS-CoV S1 protein induces strong protective immune responses against MERS-CoV infection.</p></abstract><kwd-group id="kg005"><title>Keywords</title><kwd>MERS-CoV</kwd><kwd>DNA vaccine</kwd><kwd>Spike protein</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Dongbei</li><li>Guangdong</li><li>Iowa</li><li>Jilin</li></region><settlement><li>Changchun</li><li>Iowa City</li><li>Jiangmen</li></settlement><orgName><li>Université de l'Iowa</li></orgName></list><tree><country name="République populaire de Chine"><region name="Jilin"><name sortKey="Chi, Hang" sort="Chi, Hang" uniqKey="Chi H" first="Hang" last="Chi">Hang Chi</name></region><name sortKey="Gai, Weiwei" sort="Gai, Weiwei" uniqKey="Gai W" first="Weiwei" last="Gai">Weiwei Gai</name><name sortKey="Wang, Chong" sort="Wang, Chong" uniqKey="Wang C" first="Chong" last="Wang">Chong Wang</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Xiwen" sort="Wang, Xiwen" uniqKey="Wang X" first="Xiwen" last="Wang">Xiwen Wang</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name></country><country name="États-Unis"><region name="Iowa"><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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