Antibody-mediated protection against MERS-CoV in the murine model☆
Identifieur interne : 000397 ( Pmc/Checkpoint ); précédent : 000396; suivant : 000398Antibody-mediated protection against MERS-CoV in the murine model☆
Auteurs : R. R. C. New [Royaume-Uni] ; B. D. Moore [Royaume-Uni] ; W. Butcher [Royaume-Uni] ; R. Mahood [Royaume-Uni] ; M. S. Lever [Royaume-Uni] ; S. Smither [Royaume-Uni] ; L. O'Brien [Royaume-Uni] ; S. A. Weller [Royaume-Uni] ; M. Bayliss [Royaume-Uni] ; L. C. D. Gibson [Royaume-Uni] ; C. Macleod [Royaume-Uni] ; M. Bogus [Royaume-Uni] ; R. Harvey [Royaume-Uni] ; N. Almond [Royaume-Uni] ; E. D. Williamson [Royaume-Uni]Source :
- Vaccine [ 0264-410X ] ; 2019.
Abstract
A novel dual route vaccination using the MERS-CoV RBD sub-unit has been developed. Murine antisera induced to the RBD protein , were neutralising MERS-CoV susceptibility was induced in naïve mice with Ad5hDPP4. Passive transfer of anti-RBD sera protected susceptible mice. Protected mice had a significantly reduced viral titre (P = 0.02) in their lungs.
Url:
DOI: 10.1016/j.vaccine.2019.05.074
PubMed: 31178378
PubMed Central: 7115393
Affiliations:
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<series><title level="j">Vaccine</title>
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<front><div type="abstract" xml:lang="en"><title>Highlights</title>
<p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label>
<p id="p0005">A novel dual route vaccination using the MERS-CoV RBD sub-unit has been developed.</p>
</list-item>
<list-item id="o0010"><label>•</label>
<p id="p0010">Murine antisera induced to the RBD protein , were neutralising <italic>in vitro</italic>
.</p>
</list-item>
<list-item id="o0015"><label>•</label>
<p id="p0015">MERS-CoV susceptibility was induced in naïve mice with Ad5hDPP4.</p>
</list-item>
<list-item id="o0020"><label>•</label>
<p id="p0020">Passive transfer of anti-RBD sera protected susceptible mice.</p>
</list-item>
<list-item id="o0025"><label>•</label>
<p id="p0025">Protected mice had a significantly reduced viral titre (P = 0.02) in their lungs.</p>
</list-item>
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</p>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Vaccine</journal-id>
<journal-id journal-id-type="iso-abbrev">Vaccine</journal-id>
<journal-title-group><journal-title>Vaccine</journal-title>
</journal-title-group>
<issn pub-type="ppub">0264-410X</issn>
<issn pub-type="epub">1873-2518</issn>
<publisher><publisher-name>Published by Elsevier Ltd.</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">31178378</article-id>
<article-id pub-id-type="pmc">7115393</article-id>
<article-id pub-id-type="publisher-id">S0264-410X(19)30711-X</article-id>
<article-id pub-id-type="doi">10.1016/j.vaccine.2019.05.074</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Antibody-mediated protection against MERS-CoV in the murine model<sup><xref ref-type="fn" rid="d32e1577">☆</xref>
</sup>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" id="au005"><name><surname>New</surname>
<given-names>R.R.C.</given-names>
</name>
<xref rid="af015" ref-type="aff">c</xref>
<xref rid="af030" ref-type="aff">f</xref>
</contrib>
<contrib contrib-type="author" id="au010"><name><surname>Moore</surname>
<given-names>B.D.</given-names>
</name>
<xref rid="af010" ref-type="aff">b</xref>
</contrib>
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<given-names>W.</given-names>
</name>
<xref rid="af005" ref-type="aff">a</xref>
</contrib>
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<given-names>R.</given-names>
</name>
<xref rid="af005" ref-type="aff">a</xref>
</contrib>
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<given-names>M.S.</given-names>
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<xref rid="af005" ref-type="aff">a</xref>
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<given-names>S.</given-names>
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<xref rid="af005" ref-type="aff">a</xref>
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<given-names>L.</given-names>
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<xref rid="af005" ref-type="aff">a</xref>
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<xref rid="af005" ref-type="aff">a</xref>
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<given-names>M.</given-names>
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<xref rid="af005" ref-type="aff">a</xref>
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<xref rid="af020" ref-type="aff">d</xref>
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<given-names>C.</given-names>
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<given-names>M.</given-names>
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<xref rid="af015" ref-type="aff">c</xref>
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<given-names>R.</given-names>
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<xref rid="af025" ref-type="aff">e</xref>
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<given-names>N.</given-names>
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<xref rid="af025" ref-type="aff">e</xref>
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<contrib contrib-type="author" id="au075"><name><surname>Williamson</surname>
<given-names>E.D.</given-names>
</name>
<email>dewilliamson@dstl.gov.uk</email>
<xref rid="af005" ref-type="aff">a</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
</contrib-group>
<aff id="af005"><label>a</label>
Defence Science & Technology Laboratory, Porton Down, Salisbury, UK</aff>
<aff id="af010"><label>b</label>
Pure & Applied Chemistry, University of Strathclyde, Glasgow, UK</aff>
<aff id="af015"><label>c</label>
Vaxcine (UK) Ltd, c/o London Bioscience Innovation Centre, London, UK</aff>
<aff id="af020"><label>d</label>
Scottish Biomedical Drug Discovery, West of Scotland Science Park, Glasgow, UK</aff>
<aff id="af025"><label>e</label>
National Institute for Biological Standards and Control, Potters Bar, UK</aff>
<aff id="af030"><label>f</label>
Faculty of Science and Technology, Middlesex University, The Burroughs, London, UK</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author. <email>dewilliamson@dstl.gov.uk</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>6</day>
<month>6</month>
<year>2019</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><day>9</day>
<month>7</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub"><day>6</day>
<month>6</month>
<year>2019</year>
</pub-date>
<volume>37</volume>
<issue>30</issue>
<fpage>4094</fpage>
<lpage>4102</lpage>
<history><date date-type="received"><day>20</day>
<month>12</month>
<year>2018</year>
</date>
<date date-type="rev-recd"><day>5</day>
<month>3</month>
<year>2019</year>
</date>
<date date-type="accepted"><day>26</day>
<month>5</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>Crown Copyright © 2019 Published by Elsevier Ltd.</copyright-statement>
<copyright-year>2019</copyright-year>
<copyright-holder></copyright-holder>
<license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p>
</license>
</permissions>
<abstract abstract-type="author-highlights" id="ab005"><title>Highlights</title>
<p><list list-type="simple" id="l0005"><list-item id="o0005"><label>•</label>
<p id="p0005">A novel dual route vaccination using the MERS-CoV RBD sub-unit has been developed.</p>
</list-item>
<list-item id="o0010"><label>•</label>
<p id="p0010">Murine antisera induced to the RBD protein , were neutralising <italic>in vitro</italic>
.</p>
</list-item>
<list-item id="o0015"><label>•</label>
<p id="p0015">MERS-CoV susceptibility was induced in naïve mice with Ad5hDPP4.</p>
</list-item>
<list-item id="o0020"><label>•</label>
<p id="p0020">Passive transfer of anti-RBD sera protected susceptible mice.</p>
</list-item>
<list-item id="o0025"><label>•</label>
<p id="p0025">Protected mice had a significantly reduced viral titre (P = 0.02) in their lungs.</p>
</list-item>
</list>
</p>
</abstract>
<abstract id="ab010"><p>Murine antisera with neutralising activity for the coronavirus causative of Middle East respiratory syndrome (MERS) were induced by immunisation of Balb/c mice with the receptor binding domain (RBD) of the viral Spike protein. The murine antisera induced were fully-neutralising <italic>in vitro</italic>
for two separate clinical strains of the MERS coronavirus (MERS-CoV). To test the neutralising capacity of these antisera <italic>in vivo</italic>
, susceptibility to MERS-CoV was induced in naive recipient Balb/c mice by the administration of an adenovirus vector expressing the human DPP4 receptor (Ad5-hDPP4) for MERS-CoV, prior to the passive transfer of the RBD-specific murine antisera to the transduced mice. Subsequent challenge of the recipient transduced mice by the intra-nasal route with a clinical isolate of the MERS-CoV resulted in a significantly reduced viral load in their lungs, compared with transduced mice receiving a negative control antibody. The murine antisera used were derived from mice which had been primed sub-cutaneously with a recombinant fusion of RBD with a human IgG Fc tag (RBD-Fc), adsorbed to calcium phosphate microcrystals and then boosted by the oral route with the same fusion protein in reverse micelles. The data gained indicate that this dual-route vaccination with novel formulations of the RBD-Fc, induced systemic and mucosal anti-viral immunity with demonstrated <italic>in vitro</italic>
and <italic>in vivo</italic>
neutralisation capacity for clinical strains of MERS-CoV.</p>
</abstract>
<kwd-group id="kg005"><title>Keywords</title>
<kwd>MERS</kwd>
<kwd>Coronavirus</kwd>
<kwd>Vaccination</kwd>
<kwd>Mucosal immunity</kwd>
<kwd>Systemic immunity</kwd>
<kwd>Respiratory infection</kwd>
<kwd>Neutralising antibody</kwd>
<kwd>Novel vaccine formulation</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Grand Londres</li>
</region>
<settlement><li>Londres</li>
</settlement>
</list>
<tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="New, R R C" sort="New, R R C" uniqKey="New R" first="R. R. C." last="New">R. R. C. New</name>
</region>
<name sortKey="Almond, N" sort="Almond, N" uniqKey="Almond N" first="N." last="Almond">N. Almond</name>
<name sortKey="Bayliss, M" sort="Bayliss, M" uniqKey="Bayliss M" first="M." last="Bayliss">M. Bayliss</name>
<name sortKey="Bogus, M" sort="Bogus, M" uniqKey="Bogus M" first="M." last="Bogus">M. Bogus</name>
<name sortKey="Butcher, W" sort="Butcher, W" uniqKey="Butcher W" first="W." last="Butcher">W. Butcher</name>
<name sortKey="Gibson, L C D" sort="Gibson, L C D" uniqKey="Gibson L" first="L. C. D." last="Gibson">L. C. D. Gibson</name>
<name sortKey="Harvey, R" sort="Harvey, R" uniqKey="Harvey R" first="R." last="Harvey">R. Harvey</name>
<name sortKey="Lever, M S" sort="Lever, M S" uniqKey="Lever M" first="M. S." last="Lever">M. S. Lever</name>
<name sortKey="Macleod, C" sort="Macleod, C" uniqKey="Macleod C" first="C." last="Macleod">C. Macleod</name>
<name sortKey="Mahood, R" sort="Mahood, R" uniqKey="Mahood R" first="R." last="Mahood">R. Mahood</name>
<name sortKey="Moore, B D" sort="Moore, B D" uniqKey="Moore B" first="B. D." last="Moore">B. D. Moore</name>
<name sortKey="New, R R C" sort="New, R R C" uniqKey="New R" first="R. R. C." last="New">R. R. C. New</name>
<name sortKey="O Brien, L" sort="O Brien, L" uniqKey="O Brien L" first="L." last="O'Brien">L. O'Brien</name>
<name sortKey="Smither, S" sort="Smither, S" uniqKey="Smither S" first="S." last="Smither">S. Smither</name>
<name sortKey="Weller, S A" sort="Weller, S A" uniqKey="Weller S" first="S. A." last="Weller">S. A. Weller</name>
<name sortKey="Williamson, E D" sort="Williamson, E D" uniqKey="Williamson E" first="E. D." last="Williamson">E. D. Williamson</name>
</country>
</tree>
</affiliations>
</record>
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