Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Identifieur interne : 001B39 ( Ncbi/Merge ); précédent : 001B38; suivant : 001B40Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Auteurs : Bengt Rönnberg ; Olli Vapalahti ; Marco Goeijenbier ; Chantal Reusken ; Ke Gustafsson ; Jonas Blomberg ; Ke LundkvistSource :
- Infection Ecology & Epidemiology [ 2000-8686 ] ; 2017.
Abstract
Url:
DOI: 10.1080/20008686.2017.1350086
PubMed: 28815001
PubMed Central: 5549826
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation><nlm:aff id="AFF0004"></nlm:aff></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28815001</idno><idno type="pmc">5549826</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549826</idno><idno type="RBID">PMC:5549826</idno><idno type="doi">10.1080/20008686.2017.1350086</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000D23</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D23</idno><idno type="wicri:Area/Pmc/Curation">000D23</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D23</idno><idno type="wicri:Area/Pmc/Checkpoint">000726</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000726</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:28815001</idno><idno type="wicri:Area/PubMed/Corpus">000B96</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000B96</idno><idno type="wicri:Area/PubMed/Curation">000B96</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000B96</idno><idno type="wicri:Area/PubMed/Checkpoint">000B38</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000B38</idno><idno type="wicri:Area/Ncbi/Merge">001B39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation><nlm:aff id="AFF0004"></nlm:aff></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author></analytic><series><title level="j">Infection Ecology & Epidemiology</title><idno type="eISSN">2000-8686</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p><bold>Introduction</bold>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p><p><bold>Materials and methods</bold>: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p><p><bold>Results and discussion</bold>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p><p><bold>Conclusion</bold>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. 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Lundkvist</name></author><author><name sortKey="Verner Carlsson, J" uniqKey="Verner Carlsson J">J Verner-Carlsson</name></author><author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ling, J" uniqKey="Ling J">J Ling</name></author><author><name sortKey="Vaheri, A" uniqKey="Vaheri A">A Vaheri</name></author><author><name sortKey="Hepojoki, S" uniqKey="Hepojoki S">S Hepojoki</name></author></analytic></biblStruct></listBibl></div1></back></TEI><double pmid="28815001"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation><nlm:aff id="AFF0004"></nlm:aff></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28815001</idno><idno type="pmc">5549826</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549826</idno><idno type="RBID">PMC:5549826</idno><idno type="doi">10.1080/20008686.2017.1350086</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000D23</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D23</idno><idno type="wicri:Area/Pmc/Curation">000D23</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D23</idno><idno type="wicri:Area/Pmc/Checkpoint">000726</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000726</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation><nlm:aff id="AFF0004"></nlm:aff></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author></analytic><series><title level="j">Infection Ecology & Epidemiology</title><idno type="eISSN">2000-8686</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p><bold>Introduction</bold>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p><p><bold>Materials and methods</bold>: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p><p><bold>Results and discussion</bold>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p><p><bold>Conclusion</bold>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. 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Lundkvist</name></author><author><name sortKey="Verner Carlsson, J" uniqKey="Verner Carlsson J">J Verner-Carlsson</name></author><author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ling, J" uniqKey="Ling J">J Ling</name></author><author><name sortKey="Vaheri, A" uniqKey="Vaheri A">A Vaheri</name></author><author><name sortKey="Hepojoki, S" uniqKey="Hepojoki S">S Hepojoki</name></author></analytic></biblStruct></listBibl></div1></back></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation wicri:level="4"><nlm:affiliation>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Biosciences and Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Veterinary Biosciences and Virology, University of Helsinki and Helsinki University Hospital, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation wicri:level="3"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="3"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Laboratory of Clinical Microbiology, Uppsala University Hospital, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation wicri:level="4"><nlm:affiliation>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation wicri:level="4"><nlm:affiliation>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28815001</idno><idno type="pmid">28815001</idno><idno type="doi">10.1080/20008686.2017.1350086</idno><idno type="wicri:Area/PubMed/Corpus">000B96</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000B96</idno><idno type="wicri:Area/PubMed/Curation">000B96</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000B96</idno><idno type="wicri:Area/PubMed/Checkpoint">000B38</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000B38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array.</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation wicri:level="4"><nlm:affiliation>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Biosciences and Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Veterinary Biosciences and Virology, University of Helsinki and Helsinki University Hospital, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation wicri:level="3"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="3"><nlm:affiliation>Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Clinical Microbiology, Uppsala University Hospital, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Laboratory of Clinical Microbiology, Uppsala University Hospital, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation wicri:level="4"><nlm:affiliation>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation wicri:level="4"><nlm:affiliation>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author></analytic><series><title level="j">Infection ecology & epidemiology</title><idno type="ISSN">2000-8686</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><b>Introduction</b>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome. <b>Materials and methods</b>: 'Megapeptides', 119-120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT). <b>Results and discussion</b>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations. <b>Conclusion</b>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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