Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Identifieur interne : 000726 ( Pmc/Checkpoint ); précédent : 000725; suivant : 000727Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Auteurs : Bengt Rönnberg ; Olli Vapalahti ; Marco Goeijenbier ; Chantal Reusken ; Ke Gustafsson ; Jonas Blomberg ; Ke LundkvistSource :
- Infection Ecology & Epidemiology [ 2000-8686 ] ; 2017.
Abstract
Url:
DOI: 10.1080/20008686.2017.1350086
PubMed: 28815001
PubMed Central: 5549826
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title>
<author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name>
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<author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name>
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<author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name>
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<author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name>
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<author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name>
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<author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name>
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<author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name>
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<series><title level="j">Infection Ecology & Epidemiology</title>
<idno type="eISSN">2000-8686</idno>
<imprint><date when="2017">2017</date>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p><bold>Introduction</bold>
: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p>
<p><bold>Materials and methods</bold>
: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p>
<p><bold>Results and discussion</bold>
: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p>
<p><bold>Conclusion</bold>
: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p>
</div>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Ecol Epidemiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Infect Ecol Epidemiol</journal-id>
<journal-id journal-id-type="archive">ZIEE</journal-id>
<journal-id journal-id-type="publisher-id">ziee20</journal-id>
<journal-title-group><journal-title>Infection Ecology & Epidemiology</journal-title>
</journal-title-group>
<issn pub-type="epub">2000-8686</issn>
<publisher><publisher-name>Taylor & Francis</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">28815001</article-id>
<article-id pub-id-type="pmc">5549826</article-id>
<article-id pub-id-type="publisher-id">1350086</article-id>
<article-id pub-id-type="doi">10.1080/20008686.2017.1350086</article-id>
<article-categories><subj-group subj-group-type="article-type"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Rönnberg</surname>
<given-names>Bengt</given-names>
</name>
<xref ref-type="aff" rid="AFF0001"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="AFF0002"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="AFF0003"><sup>c</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-0229-093X</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Vapalahti</surname>
<given-names>Olli</given-names>
</name>
<xref ref-type="aff" rid="AFF0004"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Goeijenbier</surname>
<given-names>Marco</given-names>
</name>
<xref ref-type="aff" rid="AFF0005"><sup>e</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-3165-8193</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Reusken</surname>
<given-names>Chantal</given-names>
</name>
<xref ref-type="aff" rid="AFF0005"><sup>e</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gustafsson</surname>
<given-names>Åke</given-names>
</name>
<xref ref-type="aff" rid="AFF0003"><sup>c</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-1472-1373</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Blomberg</surname>
<given-names>Jonas</given-names>
</name>
<xref ref-type="aff" rid="AFF0001"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="AFF0002"><sup>b</sup>
</xref>
<xref rid="FT0001" ref-type="author-notes"></xref>
<xref ref-type="corresp" rid="AN0001"><sup>*</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-6492-2491</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Lundkvist</surname>
<given-names>Åke</given-names>
</name>
<xref ref-type="aff" rid="AFF0001"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="AFF0002"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="AFF0003"><sup>c</sup>
</xref>
<xref rid="FT0001" ref-type="author-notes"></xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-1472-1373</contrib-id>
</contrib>
<aff id="AFF0001"><label><sup>a</sup>
</label>
<institution><named-content content-type="department">Section of Clinical Microbiology, Department of Medical Sciences</named-content>
,<named-content content-type="institution-name">Uppsala University</named-content>
</institution>
,<named-content content-type="city">Uppsala</named-content>
,<country>Sweden</country>
</aff>
<aff id="AFF0002"><label><sup>b</sup>
</label>
<institution><named-content content-type="department">Zoonosis Science Center, Department of Medical Biochemistry and Microbiology</named-content>
,<named-content content-type="institution-name">Uppsala University</named-content>
</institution>
,<named-content content-type="city">Uppsala</named-content>
,<country>Sweden</country>
</aff>
<aff id="AFF0003"><label><sup>c</sup>
</label>
<institution><named-content content-type="department">Laboratory of Clinical Microbiology</named-content>
,<named-content content-type="institution-name">Uppsala University Hospital</named-content>
</institution>
,<named-content content-type="city">Uppsala</named-content>
,<country>Sweden</country>
</aff>
<aff id="AFF0004"><label><sup>d</sup>
</label>
<institution><named-content content-type="department">Department of Veterinary Biosciences and Virology</named-content>
,<named-content content-type="institution-name">University of Helsinki and Helsinki University Hospital</named-content>
</institution>
,<named-content content-type="city">Helsinki</named-content>
,<country>Finland</country>
</aff>
<aff id="AFF0005"><label><sup>e</sup>
</label>
<institution><named-content content-type="department">Department of Viroscience</named-content>
,<named-content content-type="institution-name">Erasmus MC</named-content>
</institution>
,<named-content content-type="city">Rotterdam</named-content>
,<country>The Netherlands</country>
</aff>
</contrib-group>
<author-notes><corresp id="AN0001">CONTACT Jonas Blomberg <email xlink:href="Jonas.blomberg@medsci.uu.se">Jonas.blomberg@medsci.uu.se</email>
<institution><named-content content-type="department">Section of Clinical Microbiology, Department of Medical Sciences</named-content>
, <named-content content-type="institution-name">Academic Hospital</named-content>
</institution>
, <named-content content-type="postal-code">S-75185</named-content>
<named-content content-type="city">Uppsala</named-content>
, <country>Sweden</country>
</corresp>
<fn id="FT0001"><label>*</label>
<p>These authors contributed equally.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2017</year>
</pub-date>
<pub-date pub-type="epub"><day>26</day>
<month>7</month>
<year>2017</year>
</pub-date>
<volume>7</volume>
<issue>1</issue>
<elocation-id>1350086</elocation-id>
<history><date date-type="received"><day>4</day>
<month>1</month>
<year>2017</year>
</date>
<date date-type="accepted"><day>24</day>
<month>6</month>
<year>2017</year>
</date>
</history>
<permissions><copyright-statement>© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.</copyright-statement>
<copyright-year>2017</copyright-year>
<copyright-holder>The Author(s)</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<self-uri content-type="pdf" xlink:type="simple" xlink:href="ziee-7-1350086.pdf"></self-uri>
<abstract><title>ABSTRACT</title>
<p><bold>Introduction</bold>
: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p>
<p><bold>Materials and methods</bold>
: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p>
<p><bold>Results and discussion</bold>
: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p>
<p><bold>Conclusion</bold>
: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p>
</abstract>
<kwd-group kwd-group-type="author"><title>KEYWORDS</title>
<kwd>Hantavirus</kwd>
<kwd>laboratory surveillance</kwd>
<kwd>suspension multiplex immunoassay</kwd>
<kwd>pathogen surveillance</kwd>
<kwd>megapeptide</kwd>
<kwd>synthetic antigen</kwd>
<kwd>viral haemorrhagic fever</kwd>
<kwd>zoonoses</kwd>
<kwd>emerging or re-emerging diseases</kwd>
</kwd-group>
<funding-group><award-group><funding-source><named-content content-type="funder-name">Academic Hospital, Uppsala</named-content>
</funding-source>
<award-id>ALF2016</award-id>
</award-group>
<funding-statement>This work was supported by the Academic Hospital, Uppsala.</funding-statement>
</funding-group>
<counts><fig-count count="6"></fig-count>
<table-count count="1"></table-count>
<ref-count count="51"></ref-count>
<page-count count="12"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name>
<name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name>
<name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name>
<name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name>
<name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name>
<name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name>
<name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name>
</noCountry>
</tree>
</affiliations>
</record>
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