Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Identifieur interne : 000726 ( Pmc/Checkpoint ); précédent : 000725; suivant : 000727Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Auteurs : Bengt Rönnberg ; Olli Vapalahti ; Marco Goeijenbier ; Chantal Reusken ; Ke Gustafsson ; Jonas Blomberg ; Ke LundkvistSource :
- Infection Ecology & Epidemiology [ 2000-8686 ] ; 2017.
Abstract
Url:
DOI: 10.1080/20008686.2017.1350086
PubMed: 28815001
PubMed Central: 5549826
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation><nlm:aff id="AFF0004"></nlm:aff></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28815001</idno><idno type="pmc">5549826</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549826</idno><idno type="RBID">PMC:5549826</idno><idno type="doi">10.1080/20008686.2017.1350086</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000D23</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D23</idno><idno type="wicri:Area/Pmc/Curation">000D23</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D23</idno><idno type="wicri:Area/Pmc/Checkpoint">000726</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000726</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title><author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name><affiliation><nlm:aff id="AFF0004"></nlm:aff></affiliation></author><author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation><nlm:aff id="AFF0005"></nlm:aff></affiliation></author><author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author><author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation></author><author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><affiliation><nlm:aff id="AFF0001"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0002"></nlm:aff></affiliation><affiliation><nlm:aff id="AFF0003"></nlm:aff></affiliation></author></analytic><series><title level="j">Infection Ecology & Epidemiology</title><idno type="eISSN">2000-8686</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p><bold>Introduction</bold>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p><p><bold>Materials and methods</bold>: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p><p><bold>Results and discussion</bold>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p><p><bold>Conclusion</bold>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Ecol Epidemiol</journal-id><journal-id journal-id-type="iso-abbrev">Infect Ecol Epidemiol</journal-id><journal-id journal-id-type="archive">ZIEE</journal-id><journal-id journal-id-type="publisher-id">ziee20</journal-id><journal-title-group><journal-title>Infection Ecology & Epidemiology</journal-title></journal-title-group><issn pub-type="epub">2000-8686</issn><publisher><publisher-name>Taylor & Francis</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28815001</article-id><article-id pub-id-type="pmc">5549826</article-id><article-id pub-id-type="publisher-id">1350086</article-id><article-id pub-id-type="doi">10.1080/20008686.2017.1350086</article-id><article-categories><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Rönnberg</surname><given-names>Bengt</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>a</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>b</sup></xref><xref ref-type="aff" rid="AFF0003"><sup>c</sup></xref><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-0229-093X</contrib-id></contrib><contrib contrib-type="author"><name><surname>Vapalahti</surname><given-names>Olli</given-names></name><xref ref-type="aff" rid="AFF0004"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Goeijenbier</surname><given-names>Marco</given-names></name><xref ref-type="aff" rid="AFF0005"><sup>e</sup></xref><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-3165-8193</contrib-id></contrib><contrib contrib-type="author"><name><surname>Reusken</surname><given-names>Chantal</given-names></name><xref ref-type="aff" rid="AFF0005"><sup>e</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gustafsson</surname><given-names>Åke</given-names></name><xref ref-type="aff" rid="AFF0003"><sup>c</sup></xref><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-1472-1373</contrib-id></contrib><contrib contrib-type="author"><name><surname>Blomberg</surname><given-names>Jonas</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>a</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>b</sup></xref><xref rid="FT0001" ref-type="author-notes"></xref><xref ref-type="corresp" rid="AN0001"><sup>*</sup></xref><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-6492-2491</contrib-id></contrib><contrib contrib-type="author"><name><surname>Lundkvist</surname><given-names>Åke</given-names></name><xref ref-type="aff" rid="AFF0001"><sup>a</sup></xref><xref ref-type="aff" rid="AFF0002"><sup>b</sup></xref><xref ref-type="aff" rid="AFF0003"><sup>c</sup></xref><xref rid="FT0001" ref-type="author-notes"></xref><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-1472-1373</contrib-id></contrib><aff id="AFF0001"><label><sup>a</sup></label><institution><named-content content-type="department">Section of Clinical Microbiology, Department of Medical Sciences</named-content>,<named-content content-type="institution-name">Uppsala University</named-content></institution>,<named-content content-type="city">Uppsala</named-content>,<country>Sweden</country></aff><aff id="AFF0002"><label><sup>b</sup></label><institution><named-content content-type="department">Zoonosis Science Center, Department of Medical Biochemistry and Microbiology</named-content>,<named-content content-type="institution-name">Uppsala University</named-content></institution>,<named-content content-type="city">Uppsala</named-content>,<country>Sweden</country></aff><aff id="AFF0003"><label><sup>c</sup></label><institution><named-content content-type="department">Laboratory of Clinical Microbiology</named-content>,<named-content content-type="institution-name">Uppsala University Hospital</named-content></institution>,<named-content content-type="city">Uppsala</named-content>,<country>Sweden</country></aff><aff id="AFF0004"><label><sup>d</sup></label><institution><named-content content-type="department">Department of Veterinary Biosciences and Virology</named-content>,<named-content content-type="institution-name">University of Helsinki and Helsinki University Hospital</named-content></institution>,<named-content content-type="city">Helsinki</named-content>,<country>Finland</country></aff><aff id="AFF0005"><label><sup>e</sup></label><institution><named-content content-type="department">Department of Viroscience</named-content>,<named-content content-type="institution-name">Erasmus MC</named-content></institution>,<named-content content-type="city">Rotterdam</named-content>,<country>The Netherlands</country></aff></contrib-group><author-notes><corresp id="AN0001">CONTACT Jonas Blomberg <email xlink:href="Jonas.blomberg@medsci.uu.se">Jonas.blomberg@medsci.uu.se</email><institution><named-content content-type="department">Section of Clinical Microbiology, Department of Medical Sciences</named-content>, <named-content content-type="institution-name">Academic Hospital</named-content></institution>, <named-content content-type="postal-code">S-75185</named-content><named-content content-type="city">Uppsala</named-content>, <country>Sweden</country></corresp><fn id="FT0001"><label>*</label><p>These authors contributed equally.</p></fn></author-notes><pub-date pub-type="collection"><year>2017</year></pub-date><pub-date pub-type="epub"><day>26</day><month>7</month><year>2017</year></pub-date><volume>7</volume><issue>1</issue><elocation-id>1350086</elocation-id><history><date date-type="received"><day>4</day><month>1</month><year>2017</year></date><date date-type="accepted"><day>24</day><month>6</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.</copyright-statement><copyright-year>2017</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="ziee-7-1350086.pdf"></self-uri><abstract><title>ABSTRACT</title><p><bold>Introduction</bold>: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p><p><bold>Materials and methods</bold>: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p><p><bold>Results and discussion</bold>: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p><p><bold>Conclusion</bold>: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p></abstract><kwd-group kwd-group-type="author"><title>KEYWORDS</title><kwd>Hantavirus</kwd><kwd>laboratory surveillance</kwd><kwd>suspension multiplex immunoassay</kwd><kwd>pathogen surveillance</kwd><kwd>megapeptide</kwd><kwd>synthetic antigen</kwd><kwd>viral haemorrhagic fever</kwd><kwd>zoonoses</kwd><kwd>emerging or re-emerging diseases</kwd></kwd-group><funding-group><award-group><funding-source><named-content content-type="funder-name">Academic Hospital, Uppsala</named-content></funding-source><award-id>ALF2016</award-id></award-group><funding-statement>This work was supported by the Academic Hospital, Uppsala.</funding-statement></funding-group><counts><fig-count count="6"></fig-count><table-count count="1"></table-count><ref-count count="51"></ref-count><page-count count="12"></page-count></counts></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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