Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Identifieur interne : 000D23 ( Pmc/Curation ); précédent : 000D22; suivant : 000D24Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array
Auteurs : Bengt Rönnberg ; Olli Vapalahti ; Marco Goeijenbier ; Chantal Reusken ; Ke Gustafsson ; Jonas Blomberg ; Ke LundkvistSource :
- Infection Ecology & Epidemiology [ 2000-8686 ] ; 2017.
Abstract
Url:
DOI: 10.1080/20008686.2017.1350086
PubMed: 28815001
PubMed Central: 5549826
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000D23
Links to Exploration step
PMC:5549826Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title>
<author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name>
<affiliation><nlm:aff id="AFF0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0002"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name>
<affiliation><nlm:aff id="AFF0004"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name>
<affiliation><nlm:aff id="AFF0005"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name>
<affiliation><nlm:aff id="AFF0005"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name>
<affiliation><nlm:aff id="AFF0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name>
<affiliation><nlm:aff id="AFF0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name>
<affiliation><nlm:aff id="AFF0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0002"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0003"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">28815001</idno>
<idno type="pmc">5549826</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5549826</idno>
<idno type="RBID">PMC:5549826</idno>
<idno type="doi">10.1080/20008686.2017.1350086</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">000D23</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D23</idno>
<idno type="wicri:Area/Pmc/Curation">000D23</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D23</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</title>
<author><name sortKey="Ronnberg, Bengt" sort="Ronnberg, Bengt" uniqKey="Ronnberg B" first="Bengt" last="Rönnberg">Bengt Rönnberg</name>
<affiliation><nlm:aff id="AFF0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0002"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Vapalahti, Olli" sort="Vapalahti, Olli" uniqKey="Vapalahti O" first="Olli" last="Vapalahti">Olli Vapalahti</name>
<affiliation><nlm:aff id="AFF0004"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Goeijenbier, Marco" sort="Goeijenbier, Marco" uniqKey="Goeijenbier M" first="Marco" last="Goeijenbier">Marco Goeijenbier</name>
<affiliation><nlm:aff id="AFF0005"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name>
<affiliation><nlm:aff id="AFF0005"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gustafsson, Ke" sort="Gustafsson, Ke" uniqKey="Gustafsson " first=" Ke" last="Gustafsson"> Ke Gustafsson</name>
<affiliation><nlm:aff id="AFF0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Blomberg, Jonas" sort="Blomberg, Jonas" uniqKey="Blomberg J" first="Jonas" last="Blomberg">Jonas Blomberg</name>
<affiliation><nlm:aff id="AFF0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lundkvist, Ke" sort="Lundkvist, Ke" uniqKey="Lundkvist " first=" Ke" last="Lundkvist"> Ke Lundkvist</name>
<affiliation><nlm:aff id="AFF0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0002"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="AFF0003"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Infection Ecology & Epidemiology</title>
<idno type="eISSN">2000-8686</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<p><bold>Introduction</bold>
: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p>
<p><bold>Materials and methods</bold>
: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p>
<p><bold>Results and discussion</bold>
: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p>
<p><bold>Conclusion</bold>
: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Vaheri, A" uniqKey="Vaheri A">A Vaheri</name>
</author>
<author><name sortKey="Henttonen, H" uniqKey="Henttonen H">H Henttonen</name>
</author>
<author><name sortKey="Voutilainen, L" uniqKey="Voutilainen L">L Voutilainen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holmes, Ec" uniqKey="Holmes E">EC Holmes</name>
</author>
<author><name sortKey="Zhang, Y Z" uniqKey="Zhang Y">Y-Z. Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Heyman, P" uniqKey="Heyman P">P Heyman</name>
</author>
<author><name sortKey="Vervoort, T" uniqKey="Vervoort T">T Vervoort</name>
</author>
<author><name sortKey="Colson, P" uniqKey="Colson P">P Colson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Heyman, P" uniqKey="Heyman P">P Heyman</name>
</author>
<author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name>
</author>
<author><name sortKey="Berny, P" uniqKey="Berny P">P Berny</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Heyman, P" uniqKey="Heyman P">P Heyman</name>
</author>
<author><name sortKey="Baert, K" uniqKey="Baert K">K Baert</name>
</author>
<author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sanfeliu, I" uniqKey="Sanfeliu I">I Sanfeliu</name>
</author>
<author><name sortKey="Nogueras, Mm" uniqKey="Nogueras M">MM Nogueras</name>
</author>
<author><name sortKey="Gegundez, Mi" uniqKey="Gegundez M">MI Gegúndez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lin, X D" uniqKey="Lin X">X-D Lin</name>
</author>
<author><name sortKey="Guo, W P" uniqKey="Guo W">W-P Guo</name>
</author>
<author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name>
</author>
<author><name sortKey="Heyman, P" uniqKey="Heyman P">P Heyman</name>
</author>
<author><name sortKey="Baert, K" uniqKey="Baert K">K Baert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Clement, J" uniqKey="Clement J">J Clement</name>
</author>
<author><name sortKey="Maes, P" uniqKey="Maes P">P Maes</name>
</author>
<author><name sortKey="Van Ranst, M" uniqKey="Van Ranst M">M Van Ranst</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Christova, I" uniqKey="Christova I">I Christova</name>
</author>
<author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name>
</author>
<author><name sortKey="Gladnishka, T" uniqKey="Gladnishka T">T Gladnishka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goeijenbier, M" uniqKey="Goeijenbier M">M Goeijenbier</name>
</author>
<author><name sortKey="Verner Carlsson, J" uniqKey="Verner Carlsson J">J Verner-Carlsson</name>
</author>
<author><name sortKey="Van Gorp, Ec" uniqKey="Van Gorp E">EC van Gorp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Verner Carlsson, J" uniqKey="Verner Carlsson J">J Verner-Carlsson</name>
</author>
<author><name sortKey="Lohmus, M" uniqKey="Lohmus M">M Lohmus</name>
</author>
<author><name sortKey="Sundstrom, K" uniqKey="Sundstrom K">K Sundstrom</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Avsic Zupanc, T" uniqKey="Avsic Zupanc T">T Avsic-Zupanc</name>
</author>
<author><name sortKey="Petrovec, M" uniqKey="Petrovec M">M Petrovec</name>
</author>
<author><name sortKey="Duh, D" uniqKey="Duh D">D Duh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Voutilainen, L" uniqKey="Voutilainen L">L Voutilainen</name>
</author>
<author><name sortKey="Sironen, T" uniqKey="Sironen T">T Sironen</name>
</author>
<author><name sortKey="Tonteri, E" uniqKey="Tonteri E">E Tonteri</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Watson, Dc" uniqKey="Watson D">DC Watson</name>
</author>
<author><name sortKey="Sargianou, M" uniqKey="Sargianou M">M Sargianou</name>
</author>
<author><name sortKey="Papa, A" uniqKey="Papa A">A Papa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manigold, T" uniqKey="Manigold T">T Manigold</name>
</author>
<author><name sortKey="Vial, P" uniqKey="Vial P">P Vial</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reusken, C" uniqKey="Reusken C">C Reusken</name>
</author>
<author><name sortKey="Heyman, P" uniqKey="Heyman P">P Heyman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hartline, J" uniqKey="Hartline J">J Hartline</name>
</author>
<author><name sortKey="Mierek, C" uniqKey="Mierek C">C Mierek</name>
</author>
<author><name sortKey="Knutson, T" uniqKey="Knutson T">T Knutson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mattar, S" uniqKey="Mattar S">S Mattar</name>
</author>
<author><name sortKey="Guzman, C" uniqKey="Guzman C">C Guzmán</name>
</author>
<author><name sortKey="Figueiredo, Lt" uniqKey="Figueiredo L">LT Figueiredo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lundkvist, A" uniqKey="Lundkvist A">A Lundkvist</name>
</author>
<author><name sortKey="Meisel, H" uniqKey="Meisel H">H Meisel</name>
</author>
<author><name sortKey="Koletzki, D" uniqKey="Koletzki D">D Koletzki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Heiskanen, T" uniqKey="Heiskanen T">T Heiskanen</name>
</author>
<author><name sortKey="Lundkvist, A" uniqKey="Lundkvist A">A Lundkvist</name>
</author>
<author><name sortKey="Soliymani, R" uniqKey="Soliymani R">R Soliymani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Elfaitouri, A" uniqKey="Elfaitouri A">A Elfaitouri</name>
</author>
<author><name sortKey="Herrmann, B" uniqKey="Herrmann B">B Herrmann</name>
</author>
<author><name sortKey="Bolin Wiener, A" uniqKey="Bolin Wiener A">A Bolin-Wiener</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blomberg, J" uniqKey="Blomberg J">J Blomberg</name>
</author>
<author><name sortKey="Blomberg, F" uniqKey="Blomberg F">F Blomberg</name>
</author>
<author><name sortKey="Sjosten, A" uniqKey="Sjosten A">A Sjösten</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rizwan, M" uniqKey="Rizwan M">M Rizwan</name>
</author>
<author><name sortKey="Ronnberg, B" uniqKey="Ronnberg B">B Ronnberg</name>
</author>
<author><name sortKey="Cistjakovs, M" uniqKey="Cistjakovs M">M Cistjakovs</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Elgh, F" uniqKey="Elgh F">F Elgh</name>
</author>
<author><name sortKey="Linderholm, M" uniqKey="Linderholm M">M Linderholm</name>
</author>
<author><name sortKey="Wadell, G" uniqKey="Wadell G">G Wadell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yoshimatsu, K" uniqKey="Yoshimatsu K">K Yoshimatsu</name>
</author>
<author><name sortKey="Arikawa, J" uniqKey="Arikawa J">J Arikawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yoshimatsu, K" uniqKey="Yoshimatsu K">K Yoshimatsu</name>
</author>
<author><name sortKey="Arikawa, J" uniqKey="Arikawa J">J Arikawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name>
</author>
<author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Kim, Y" uniqKey="Kim Y">Y Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name>
</author>
<author><name sortKey="Boudreaux, Dm" uniqKey="Boudreaux D">DM Boudreaux</name>
</author>
<author><name sortKey="Estrada, Df" uniqKey="Estrada D">DF Estrada</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goeijenbier, M" uniqKey="Goeijenbier M">M Goeijenbier</name>
</author>
<author><name sortKey="Hartskeerl, Ra" uniqKey="Hartskeerl R">RA Hartskeerl</name>
</author>
<author><name sortKey="Reimerink, J" uniqKey="Reimerink J">J Reimerink</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gherasim, A" uniqKey="Gherasim A">A Gherasim</name>
</author>
<author><name sortKey="Hjertqvist, M" uniqKey="Hjertqvist M">M Hjertqvist</name>
</author>
<author><name sortKey="Lundkvist, A" uniqKey="Lundkvist A">A Lundkvist</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lundkvist, A" uniqKey="Lundkvist A">A Lundkvist</name>
</author>
<author><name sortKey="Kallio Kokko, H" uniqKey="Kallio Kokko H">H Kallio-Kokko</name>
</author>
<author><name sortKey="Sjolander, Kb" uniqKey="Sjolander K">KB Sjölander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kallio Kokko, H" uniqKey="Kallio Kokko H">H Kallio-Kokko</name>
</author>
<author><name sortKey="Leveelahti, R" uniqKey="Leveelahti R">R Leveelahti</name>
</author>
<author><name sortKey="Brummer Korvenkontio, M" uniqKey="Brummer Korvenkontio M">M Brummer-Korvenkontio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hedman, K" uniqKey="Hedman K">K Hedman</name>
</author>
<author><name sortKey="Vaheri, A" uniqKey="Vaheri A">A Vaheri</name>
</author>
<author><name sortKey="Brummer Korvenkontio, M" uniqKey="Brummer Korvenkontio M">M Brummer-Korvenkontio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sjolander, Kb" uniqKey="Sjolander K">KB Sjölander</name>
</author>
<author><name sortKey="Elgh, F" uniqKey="Elgh F">F Elgh</name>
</author>
<author><name sortKey="Kallio Kokko, H" uniqKey="Kallio Kokko H">H Kallio-Kokko</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hepojoki, S" uniqKey="Hepojoki S">S Hepojoki</name>
</author>
<author><name sortKey="Rusanen, J" uniqKey="Rusanen J">J Rusanen</name>
</author>
<author><name sortKey="Hepojoki, J" uniqKey="Hepojoki J">J Hepojoki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klingstrom, J" uniqKey="Klingstrom J">J Klingström</name>
</author>
<author><name sortKey="Plyusnin, A" uniqKey="Plyusnin A">A Plyusnin</name>
</author>
<author><name sortKey="Vaheri, A" uniqKey="Vaheri A">A Vaheri</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lohmus, M" uniqKey="Lohmus M">M Lohmus</name>
</author>
<author><name sortKey="Verner Carlsson, J" uniqKey="Verner Carlsson J">J Verner-Carlsson</name>
</author>
<author><name sortKey="Borg, O" uniqKey="Borg O">O Borg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haste Andersen, P" uniqKey="Haste Andersen P">P Haste Andersen</name>
</author>
<author><name sortKey="Nielsen, M" uniqKey="Nielsen M">M Nielsen</name>
</author>
<author><name sortKey="Lund, O" uniqKey="Lund O">O Lund</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vapalahti, O" uniqKey="Vapalahti O">O Vapalahti</name>
</author>
<author><name sortKey="Kallio Kokko, H" uniqKey="Kallio Kokko H">H Kallio-Kokko</name>
</author>
<author><name sortKey="N Rv Nen, A" uniqKey="N Rv Nen A">A Närvänen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dzagurova, T" uniqKey="Dzagurova T">T Dzagurova</name>
</author>
<author><name sortKey="Tkachenko, E" uniqKey="Tkachenko E">E Tkachenko</name>
</author>
<author><name sortKey="Slonova, R" uniqKey="Slonova R">R Slonova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, L" uniqKey="Xu L">L Xu</name>
</author>
<author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name>
</author>
<author><name sortKey="He, B" uniqKey="He B">B He</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Noh, Jy" uniqKey="Noh J">JY Noh</name>
</author>
<author><name sortKey="Cheong, Hj" uniqKey="Cheong H">HJ Cheong</name>
</author>
<author><name sortKey="Song, Jy" uniqKey="Song J">JY Song</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Terajima, M" uniqKey="Terajima M">M Terajima</name>
</author>
<author><name sortKey="Hendershot, Jd" uniqKey="Hendershot J">JD Hendershot</name>
</author>
<author><name sortKey="Kariwa, H" uniqKey="Kariwa H">H Kariwa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Elgh, F" uniqKey="Elgh F">F Elgh</name>
</author>
<author><name sortKey="Lundkvist, A" uniqKey="Lundkvist A">A Lundkvist</name>
</author>
<author><name sortKey="Alexeyev, Oa" uniqKey="Alexeyev O">OA Alexeyev</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Carvalho Nicacio, C" uniqKey="De Carvalho Nicacio C">C de Carvalho Nicacio</name>
</author>
<author><name sortKey="S Llberg, M" uniqKey="S Llberg M">M Sällberg</name>
</author>
<author><name sortKey="Hultgren, C" uniqKey="Hultgren C">C Hultgren</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Todd, Dj" uniqKey="Todd D">DJ Todd</name>
</author>
<author><name sortKey="Knowlton, N" uniqKey="Knowlton N">N Knowlton</name>
</author>
<author><name sortKey="Amato, M" uniqKey="Amato M">M Amato</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Waterboer, T" uniqKey="Waterboer T">T Waterboer</name>
</author>
<author><name sortKey="Sehr, P" uniqKey="Sehr P">P Sehr</name>
</author>
<author><name sortKey="Pawlita, M" uniqKey="Pawlita M">M Pawlita</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Razzauti, M" uniqKey="Razzauti M">M Razzauti</name>
</author>
<author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name>
</author>
<author><name sortKey="Sironen, T" uniqKey="Sironen T">T Sironen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lundkvist, A" uniqKey="Lundkvist A">A Lundkvist</name>
</author>
<author><name sortKey="Verner Carlsson, J" uniqKey="Verner Carlsson J">J Verner-Carlsson</name>
</author>
<author><name sortKey="Plyusnina, A" uniqKey="Plyusnina A">A Plyusnina</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ling, J" uniqKey="Ling J">J Ling</name>
</author>
<author><name sortKey="Vaheri, A" uniqKey="Vaheri A">A Vaheri</name>
</author>
<author><name sortKey="Hepojoki, S" uniqKey="Hepojoki S">S Hepojoki</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Ecol Epidemiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Infect Ecol Epidemiol</journal-id>
<journal-id journal-id-type="archive">ZIEE</journal-id>
<journal-id journal-id-type="publisher-id">ziee20</journal-id>
<journal-title-group><journal-title>Infection Ecology & Epidemiology</journal-title>
</journal-title-group>
<issn pub-type="epub">2000-8686</issn>
<publisher><publisher-name>Taylor & Francis</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">28815001</article-id>
<article-id pub-id-type="pmc">5549826</article-id>
<article-id pub-id-type="publisher-id">1350086</article-id>
<article-id pub-id-type="doi">10.1080/20008686.2017.1350086</article-id>
<article-categories><subj-group subj-group-type="article-type"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Rönnberg</surname>
<given-names>Bengt</given-names>
</name>
<xref ref-type="aff" rid="AFF0001"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="AFF0002"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="AFF0003"><sup>c</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-0229-093X</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Vapalahti</surname>
<given-names>Olli</given-names>
</name>
<xref ref-type="aff" rid="AFF0004"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Goeijenbier</surname>
<given-names>Marco</given-names>
</name>
<xref ref-type="aff" rid="AFF0005"><sup>e</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-3165-8193</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Reusken</surname>
<given-names>Chantal</given-names>
</name>
<xref ref-type="aff" rid="AFF0005"><sup>e</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gustafsson</surname>
<given-names>Åke</given-names>
</name>
<xref ref-type="aff" rid="AFF0003"><sup>c</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-1472-1373</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Blomberg</surname>
<given-names>Jonas</given-names>
</name>
<xref ref-type="aff" rid="AFF0001"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="AFF0002"><sup>b</sup>
</xref>
<xref rid="FT0001" ref-type="author-notes"></xref>
<xref ref-type="corresp" rid="AN0001"><sup>*</sup>
</xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-6492-2491</contrib-id>
</contrib>
<contrib contrib-type="author"><name><surname>Lundkvist</surname>
<given-names>Åke</given-names>
</name>
<xref ref-type="aff" rid="AFF0001"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="AFF0002"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="AFF0003"><sup>c</sup>
</xref>
<xref rid="FT0001" ref-type="author-notes"></xref>
<contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-1472-1373</contrib-id>
</contrib>
<aff id="AFF0001"><label><sup>a</sup>
</label>
<institution><named-content content-type="department">Section of Clinical Microbiology, Department of Medical Sciences</named-content>
,<named-content content-type="institution-name">Uppsala University</named-content>
</institution>
,<named-content content-type="city">Uppsala</named-content>
,<country>Sweden</country>
</aff>
<aff id="AFF0002"><label><sup>b</sup>
</label>
<institution><named-content content-type="department">Zoonosis Science Center, Department of Medical Biochemistry and Microbiology</named-content>
,<named-content content-type="institution-name">Uppsala University</named-content>
</institution>
,<named-content content-type="city">Uppsala</named-content>
,<country>Sweden</country>
</aff>
<aff id="AFF0003"><label><sup>c</sup>
</label>
<institution><named-content content-type="department">Laboratory of Clinical Microbiology</named-content>
,<named-content content-type="institution-name">Uppsala University Hospital</named-content>
</institution>
,<named-content content-type="city">Uppsala</named-content>
,<country>Sweden</country>
</aff>
<aff id="AFF0004"><label><sup>d</sup>
</label>
<institution><named-content content-type="department">Department of Veterinary Biosciences and Virology</named-content>
,<named-content content-type="institution-name">University of Helsinki and Helsinki University Hospital</named-content>
</institution>
,<named-content content-type="city">Helsinki</named-content>
,<country>Finland</country>
</aff>
<aff id="AFF0005"><label><sup>e</sup>
</label>
<institution><named-content content-type="department">Department of Viroscience</named-content>
,<named-content content-type="institution-name">Erasmus MC</named-content>
</institution>
,<named-content content-type="city">Rotterdam</named-content>
,<country>The Netherlands</country>
</aff>
</contrib-group>
<author-notes><corresp id="AN0001">CONTACT Jonas Blomberg <email xlink:href="Jonas.blomberg@medsci.uu.se">Jonas.blomberg@medsci.uu.se</email>
<institution><named-content content-type="department">Section of Clinical Microbiology, Department of Medical Sciences</named-content>
, <named-content content-type="institution-name">Academic Hospital</named-content>
</institution>
, <named-content content-type="postal-code">S-75185</named-content>
<named-content content-type="city">Uppsala</named-content>
, <country>Sweden</country>
</corresp>
<fn id="FT0001"><label>*</label>
<p>These authors contributed equally.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2017</year>
</pub-date>
<pub-date pub-type="epub"><day>26</day>
<month>7</month>
<year>2017</year>
</pub-date>
<volume>7</volume>
<issue>1</issue>
<elocation-id>1350086</elocation-id>
<history><date date-type="received"><day>4</day>
<month>1</month>
<year>2017</year>
</date>
<date date-type="accepted"><day>24</day>
<month>6</month>
<year>2017</year>
</date>
</history>
<permissions><copyright-statement>© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.</copyright-statement>
<copyright-year>2017</copyright-year>
<copyright-holder>The Author(s)</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<self-uri content-type="pdf" xlink:type="simple" xlink:href="ziee-7-1350086.pdf"></self-uri>
<abstract><title>ABSTRACT</title>
<p><bold>Introduction</bold>
: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome.</p>
<p><bold>Materials and methods</bold>
: ‘Megapeptides’, 119–120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT).</p>
<p><bold>Results and discussion</bold>
: The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations.</p>
<p><bold>Conclusion</bold>
: The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.</p>
</abstract>
<kwd-group kwd-group-type="author"><title>KEYWORDS</title>
<kwd>Hantavirus</kwd>
<kwd>laboratory surveillance</kwd>
<kwd>suspension multiplex immunoassay</kwd>
<kwd>pathogen surveillance</kwd>
<kwd>megapeptide</kwd>
<kwd>synthetic antigen</kwd>
<kwd>viral haemorrhagic fever</kwd>
<kwd>zoonoses</kwd>
<kwd>emerging or re-emerging diseases</kwd>
</kwd-group>
<funding-group><award-group><funding-source><named-content content-type="funder-name">Academic Hospital, Uppsala</named-content>
</funding-source>
<award-id>ALF2016</award-id>
</award-group>
<funding-statement>This work was supported by the Academic Hospital, Uppsala.</funding-statement>
</funding-group>
<counts><fig-count count="6"></fig-count>
<table-count count="1"></table-count>
<ref-count count="51"></ref-count>
<page-count count="12"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D23 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000D23 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:5549826 |texte= Serogrouping and seroepidemiology of North European hantaviruses using a novel broadly targeted synthetic nucleoprotein antigen array }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:28815001" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |