MersV1, Ncbi, Merge, bibRecord, 000377

Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.

Identifieur interne : 000377 ( Ncbi/Merge ); précédent : 000376; suivant : 000378

Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.

Auteurs : Edyta Ko Cia Ska [Grèce] ; Kriton Kalantidis ; Krzysztof Wypijewski ; Jan Sadowski ; Martin Tabler

Source :

Plant molecular biology [ 0167-4412 ] ; 2005.

RBID : pubmed:16244913

Descripteurs français

English descriptors

KwdEn :
- Agrobacterium tumefaciens (genetics), Gene Expression Regulation, Plant, Genetic Engineering, Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Plant Leaves (genetics), Plants, Genetically Modified, RNA Interference, RNA, Double-Stranded (genetics), RNA, Double-Stranded (metabolism), RNA, Plant (genetics), RNA, Plant (metabolism), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), Tobacco (classification), Tobacco (genetics), Transgenes (genetics).
MESH :
- chemical , genetics : Green Fluorescent Proteins, RNA, Double-Stranded, RNA, Plant, RNA, Small Interfering.
- classification : Tobacco.
- genetics : Agrobacterium tumefaciens, Plant Leaves, Tobacco, Transgenes.
- chemical , metabolism : Green Fluorescent Proteins, RNA, Double-Stranded, RNA, Plant, RNA, Small Interfering.
- Gene Expression Regulation, Plant, Genetic Engineering, Plants, Genetically Modified, RNA Interference.

Abstract

In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen during systemic silencing. More specifically, infiltration of two transgene hairpin constructs resulted in elevated levels of siRNAs. However, differences between the two constructs were observed: the antisense-sense arrangement was more effective than the sense-antisense order. For both double-stranded forms, we observed a relative increase of the 24-mer size class of siRNAs. When a comparable hairpin construct of the endogenous Virp1 gene was assayed, the portion of the 24-mer siRNA class remained low as observed for all kinds of single-stranded inducers. The lack of increase of Virp1-derived 24-mers was independent of the expression level, as demonstrated by agroinfiltration into a transgenic plant that overexpressed Virp1 and showed the same pattern. Using transducer constructs, we could detect within a week transitive silencing from GFP to GUS sequences in the infiltrated zone and in either direction 5'-3' and 3'-5'. Conversely, for the endogenous Virp1 gene neither transitive silencing nor the induction of systemic silencing could be observed. These results are discussed in view of the current models of RNA silencing.

DOI: 10.1007/s11103-005-0668-x
PubMed: 16244913

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002303
to stream PubMed, to step Curation: 002303
to stream PubMed, to step Checkpoint: 002220

Links to Exploration step

pubmed:16244913

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.</title>
<author><name sortKey="Ko Cia Ska, Edyta" sort="Ko Cia Ska, Edyta" uniqKey="Ko Cia Ska E" first="Edyta" last="Ko Cia Ska">Edyta Ko Cia Ska</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, P.O. Box 1527, GR-71110 Crete, Heraklion, Greece.</nlm:affiliation>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, P.O. Box 1527, GR-71110 Crete, Heraklion</wicri:regionArea>
<wicri:noRegion>Heraklion</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kalantidis, Kriton" sort="Kalantidis, Kriton" uniqKey="Kalantidis K" first="Kriton" last="Kalantidis">Kriton Kalantidis</name>
</author>
<author><name sortKey="Wypijewski, Krzysztof" sort="Wypijewski, Krzysztof" uniqKey="Wypijewski K" first="Krzysztof" last="Wypijewski">Krzysztof Wypijewski</name>
</author>
<author><name sortKey="Sadowski, Jan" sort="Sadowski, Jan" uniqKey="Sadowski J" first="Jan" last="Sadowski">Jan Sadowski</name>
</author>
<author><name sortKey="Tabler, Martin" sort="Tabler, Martin" uniqKey="Tabler M" first="Martin" last="Tabler">Martin Tabler</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16244913</idno>
<idno type="pmid">16244913</idno>
<idno type="doi">10.1007/s11103-005-0668-x</idno>
<idno type="wicri:Area/PubMed/Corpus">002303</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002303</idno>
<idno type="wicri:Area/PubMed/Curation">002303</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002303</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002220</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002220</idno>
<idno type="wicri:Area/Ncbi/Merge">000377</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.</title>
<author><name sortKey="Ko Cia Ska, Edyta" sort="Ko Cia Ska, Edyta" uniqKey="Ko Cia Ska E" first="Edyta" last="Ko Cia Ska">Edyta Ko Cia Ska</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, P.O. Box 1527, GR-71110 Crete, Heraklion, Greece.</nlm:affiliation>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, P.O. Box 1527, GR-71110 Crete, Heraklion</wicri:regionArea>
<wicri:noRegion>Heraklion</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kalantidis, Kriton" sort="Kalantidis, Kriton" uniqKey="Kalantidis K" first="Kriton" last="Kalantidis">Kriton Kalantidis</name>
</author>
<author><name sortKey="Wypijewski, Krzysztof" sort="Wypijewski, Krzysztof" uniqKey="Wypijewski K" first="Krzysztof" last="Wypijewski">Krzysztof Wypijewski</name>
</author>
<author><name sortKey="Sadowski, Jan" sort="Sadowski, Jan" uniqKey="Sadowski J" first="Jan" last="Sadowski">Jan Sadowski</name>
</author>
<author><name sortKey="Tabler, Martin" sort="Tabler, Martin" uniqKey="Tabler M" first="Martin" last="Tabler">Martin Tabler</name>
</author>
</analytic>
<series><title level="j">Plant molecular biology</title>
<idno type="ISSN">0167-4412</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genetic Engineering</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Plant Leaves (genetics)</term>
<term>Plants, Genetically Modified</term>
<term>RNA Interference</term>
<term>RNA, Double-Stranded (genetics)</term>
<term>RNA, Double-Stranded (metabolism)</term>
<term>RNA, Plant (genetics)</term>
<term>RNA, Plant (metabolism)</term>
<term>RNA, Small Interfering (genetics)</term>
<term>RNA, Small Interfering (metabolism)</term>
<term>Tobacco (classification)</term>
<term>Tobacco (genetics)</term>
<term>Transgenes (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term>
<term>ARN des plantes (métabolisme)</term>
<term>ARN double brin (génétique)</term>
<term>ARN double brin (métabolisme)</term>
<term>Agrobacterium tumefaciens (génétique)</term>
<term>Feuilles de plante (génétique)</term>
<term>Génie génétique</term>
<term>Interférence par ARN</term>
<term>Petit ARN interférent (génétique)</term>
<term>Petit ARN interférent (métabolisme)</term>
<term>Protéines à fluorescence verte (génétique)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Tabac ()</term>
<term>Tabac (génétique)</term>
<term>Transgènes (génétique)</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term>
<term>RNA, Double-Stranded</term>
<term>RNA, Plant</term>
<term>RNA, Small Interfering</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Plant Leaves</term>
<term>Tobacco</term>
<term>Transgenes</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term>
<term>ARN double brin</term>
<term>Agrobacterium tumefaciens</term>
<term>Feuilles de plante</term>
<term>Petit ARN interférent</term>
<term>Protéines à fluorescence verte</term>
<term>Tabac</term>
<term>Transgènes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term>
<term>RNA, Double-Stranded</term>
<term>RNA, Plant</term>
<term>RNA, Small Interfering</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term>
<term>ARN double brin</term>
<term>Petit ARN interférent</term>
<term>Protéines à fluorescence verte</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Genetic Engineering</term>
<term>Plants, Genetically Modified</term>
<term>RNA Interference</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Génie génétique</term>
<term>Interférence par ARN</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Tabac</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen during systemic silencing. More specifically, infiltration of two transgene hairpin constructs resulted in elevated levels of siRNAs. However, differences between the two constructs were observed: the antisense-sense arrangement was more effective than the sense-antisense order. For both double-stranded forms, we observed a relative increase of the 24-mer size class of siRNAs. When a comparable hairpin construct of the endogenous Virp1 gene was assayed, the portion of the 24-mer siRNA class remained low as observed for all kinds of single-stranded inducers. The lack of increase of Virp1-derived 24-mers was independent of the expression level, as demonstrated by agroinfiltration into a transgenic plant that overexpressed Virp1 and showed the same pattern. Using transducer constructs, we could detect within a week transitive silencing from GFP to GUS sequences in the infiltrated zone and in either direction 5'-3' and 3'-5'. Conversely, for the endogenous Virp1 gene neither transitive silencing nor the induction of systemic silencing could be observed. These results are discussed in view of the current models of RNA silencing.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16244913</PMID>
<DateCompleted><Year>2006</Year>
<Month>01</Month>
<Day>10</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0167-4412</ISSN>
<JournalIssue CitedMedium="Print"><Volume>59</Volume>
<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Plant molecular biology</Title>
<ISOAbbreviation>Plant Mol. Biol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.</ArticleTitle>
<Pagination><MedlinePgn>647-61</MedlinePgn>
</Pagination>
<Abstract><AbstractText>In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen during systemic silencing. More specifically, infiltration of two transgene hairpin constructs resulted in elevated levels of siRNAs. However, differences between the two constructs were observed: the antisense-sense arrangement was more effective than the sense-antisense order. For both double-stranded forms, we observed a relative increase of the 24-mer size class of siRNAs. When a comparable hairpin construct of the endogenous Virp1 gene was assayed, the portion of the 24-mer siRNA class remained low as observed for all kinds of single-stranded inducers. The lack of increase of Virp1-derived 24-mers was independent of the expression level, as demonstrated by agroinfiltration into a transgenic plant that overexpressed Virp1 and showed the same pattern. Using transducer constructs, we could detect within a week transitive silencing from GFP to GUS sequences in the infiltrated zone and in either direction 5'-3' and 3'-5'. Conversely, for the endogenous Virp1 gene neither transitive silencing nor the induction of systemic silencing could be observed. These results are discussed in view of the current models of RNA silencing.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kościańska</LastName>
<ForeName>Edyta</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas, P.O. Box 1527, GR-71110 Crete, Heraklion, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kalantidis</LastName>
<ForeName>Kriton</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wypijewski</LastName>
<ForeName>Krzysztof</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Sadowski</LastName>
<ForeName>Jan</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tabler</LastName>
<ForeName>Martin</ForeName>
<Initials>M</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Plant Mol Biol</MedlineTA>
<NlmUniqueID>9106343</NlmUniqueID>
<ISSNLinking>0167-4412</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012330">RNA, Double-Stranded</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>147336-22-9</RegistryNumber>
<NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005818" MajorTopicYN="Y">Genetic Engineering</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D034622" MajorTopicYN="Y">RNA Interference</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012330" MajorTopicYN="N">RNA, Double-Stranded</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D034741" MajorTopicYN="N">RNA, Small Interfering</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year>
<Month>12</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2005</Year>
<Month>07</Month>
<Day>08</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>10</Month>
<Day>26</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>1</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>10</Month>
<Day>26</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16244913</ArticleId>
<ArticleId IdType="doi">10.1007/s11103-005-0668-x</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Plant J. 2004 Apr;38(1):107-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15053764</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Gen Genet. 1997 Nov;256(5):581-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9413443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Sep 16;431(7006):364-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15372044</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1996 Feb;8(2):179-188</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12239381</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Jan;131(1):245-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12529532</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2002 Apr;14(4):857-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11971140</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2003 Sep 1;22(17):4523-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12941703</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2003 Mar;33(5):949-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12609035</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6297-302</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15079073</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2003 Feb;16(2):149-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12575749</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 1998 Dec;16(6):651-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10069073</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2004 May 21;566(1-3):223-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15147899</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 2003 Jan 1;17(1):49-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12514099</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2003 Feb 3;22(3):633-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12554663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Sep 16;431(7006):350-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15372042</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Sep 16;431(7006):343-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15372041</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2002 Nov 1;21(21):5824-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12411500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2004 Mar;37(5):762-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14871315</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2002 Apr 1;30(7):1688-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11917031</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2003 Dec;13(12):2651-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14656969</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Jul;132(3):1382-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12857820</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2003 Feb 4;13(3):236-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12573220</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1984 Nov 26;12(22):8711-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6095209</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9632-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12886005</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2004 May;2(5):E104</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15024409</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2001 Nov 16;107(4):465-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11719187</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2002;14 Suppl:S289-301</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12045284</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2002 Aug;8(8):1034-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12212847</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2001 Jun 1;29(11):2395-400</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11376158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2003 Sep;77(17):9685-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12915580</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2004 Apr;85(Pt 4):983-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15039540</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 1992 Sep;19(6):959-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1511141</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 May 27;100(11):6343-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730368</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2002 Sep 2;21(17):4671-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12198169</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2003 Mar;9(3):299-308</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12592004</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1998 Oct 16;95(2):177-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9790525</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1997 Aug 1;16(15):4738-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9303318</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Sep 16;431(7006):356-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15372043</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Jul 8;430(6996):161-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15241403</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2002 Nov;7(11):487-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12417148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1997 Oct 9;389(6651):553</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9335491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2001 Jul;126(3):930-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11457942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2004 May;38(4):594-602</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15125766</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2002 Aug;15(8):826-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12182340</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 1992 Dec;20(6):1203-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1463857</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1998 Oct;10(10):1747-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9761800</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2004 Apr 9;563(1-3):123-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15063735</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11981-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12181491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2001 Jan;7(1):16-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11214177</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>RNA. 2003 Jan;9(1):25-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12554873</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Sep 16;431(7006):338-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15372040</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Grèce</li>
</country>
</list>
<tree><noCountry><name sortKey="Kalantidis, Kriton" sort="Kalantidis, Kriton" uniqKey="Kalantidis K" first="Kriton" last="Kalantidis">Kriton Kalantidis</name>
<name sortKey="Sadowski, Jan" sort="Sadowski, Jan" uniqKey="Sadowski J" first="Jan" last="Sadowski">Jan Sadowski</name>
<name sortKey="Tabler, Martin" sort="Tabler, Martin" uniqKey="Tabler M" first="Martin" last="Tabler">Martin Tabler</name>
<name sortKey="Wypijewski, Krzysztof" sort="Wypijewski, Krzysztof" uniqKey="Wypijewski K" first="Krzysztof" last="Wypijewski">Krzysztof Wypijewski</name>
</noCountry>
<country name="Grèce"><noRegion><name sortKey="Ko Cia Ska, Edyta" sort="Ko Cia Ska, Edyta" uniqKey="Ko Cia Ska E" first="Edyta" last="Ko Cia Ska">Edyta Ko Cia Ska</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000377 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000377 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     pubmed:16244913
   |texte=   Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:16244913" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.

Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki