Gene sensors based on peptide nucleic acid (PNA) probes: relationship between sensor sensitivity and probe/target duplex stability.
Identifieur interne : 000376 ( Ncbi/Merge ); précédent : 000375; suivant : 000377Gene sensors based on peptide nucleic acid (PNA) probes: relationship between sensor sensitivity and probe/target duplex stability.
Auteurs : Hiroshi Aoki [Japon] ; Hiroaki TaoSource :
- The Analyst [ 0003-2654 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN.
- génétique : Acides nucléiques peptidiques.
- instrumentation : Animaux, Hétéroduplexes d'acides nucléiques, Matrices (génétique), Mésappariement de bases, Séquençage par oligonucléotides en batterie, Électrochimie, Électrodes.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA.
- chemical , genetics : Peptide Nucleic Acids.
- instrumentation : Electrochemistry.
- methods : Electrochemistry.
- Animals, Base Pair Mismatch, Electrodes, Nucleic Acid Heteroduplexes, Oligonucleotide Array Sequence Analysis, Templates, Genetic.
Abstract
Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.
DOI: 10.1039/b507121f
PubMed: 16222367
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pubmed:16222367Le document en format XML
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xml:lang="en"><term>Electrochemistry</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrochemistry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Pair Mismatch</term><term>Electrodes</term><term>Nucleic Acid Heteroduplexes</term><term>Oligonucleotide Array Sequence Analysis</term><term>Templates, Genetic</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Animaux</term><term>Hétéroduplexes d'acides nucléiques</term><term>Matrices (génétique)</term><term>Mésappariement de bases</term><term>Séquençage par oligonucléotides en batterie</term><term>Électrochimie</term><term>Électrodes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16222367</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>19</Day></DateCompleted><DateRevised><Year>2005</Year><Month>10</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0003-2654</ISSN><JournalIssue CitedMedium="Print"><Volume>130</Volume><Issue>11</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The Analyst</Title><ISOAbbreviation>Analyst</ISOAbbreviation></Journal><ArticleTitle>Gene sensors based on peptide nucleic acid (PNA) probes: relationship between sensor sensitivity and probe/target duplex stability.</ArticleTitle><Pagination><MedlinePgn>1478-82</MedlinePgn></Pagination><Abstract><AbstractText>Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-, 15-, 17-, 20-, and 22-mers). It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence, and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs. In addition, by using two kinds of probe PNAs with different lengths, it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aoki</LastName><ForeName>Hiroshi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8569, Japan. aoki-h@aist.go.jp</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Hiroaki</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>08</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Analyst</MedlineTA><NlmUniqueID>0372652</NlmUniqueID><ISSNLinking>0003-2654</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009692">Nucleic Acid Heteroduplexes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020135">Peptide Nucleic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020137" MajorTopicYN="N">Base Pair Mismatch</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004563" MajorTopicYN="N">Electrochemistry</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004566" MajorTopicYN="N">Electrodes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009692" MajorTopicYN="N">Nucleic Acid Heteroduplexes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="Y">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020135" MajorTopicYN="N">Peptide Nucleic Acids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013698" MajorTopicYN="N">Templates, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16222367</ArticleId><ArticleId IdType="doi">10.1039/b507121f</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Tao, Hiroaki" sort="Tao, Hiroaki" uniqKey="Tao H" first="Hiroaki" last="Tao">Hiroaki Tao</name></noCountry><country name="Japon"><noRegion><name sortKey="Aoki, Hiroshi" sort="Aoki, Hiroshi" uniqKey="Aoki H" first="Hiroshi" last="Aoki">Hiroshi Aoki</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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