MersV1, Ncbi, Merge, bibRecord, 000378

Recent highlights on photolithic oligonucleotide array in situ synthesis.

Identifieur interne : 000378 ( Ncbi/Merge ); précédent : 000377; suivant : 000379

Recent highlights on photolithic oligonucleotide array in situ synthesis.

Auteurs : Klaus-Peter Stengele [Allemagne] ; Jochen Bühler ; Sigrid Bühler ; Evgueni Kvassiouk ; Roland Green ; Tamara Prykota ; Wolfgang Pfleiderer

Source :

Nucleosides, nucleotides & nucleic acids [ 1525-7770 ] ; 2005.

RBID : pubmed:16248056

Descripteurs français

English descriptors

KwdEn :
- Combinatorial Chemistry Techniques, Gene Expression Profiling (instrumentation), Kinetics, Models, Chemical, Molecular Biology (methods), Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis (instrumentation), Oligonucleotide Probes, Oligonucleotides (chemistry), Photochemistry, Propanols (chemistry), Time Factors, Ultraviolet Rays.
MESH :
- chemical , chemistry : Oligonucleotides, Propanols.
- chemical : Oligonucleotide Probes.
- instrumentation : Gene Expression Profiling, Oligonucleotide Array Sequence Analysis.
- methods : Molecular Biology.
- Combinatorial Chemistry Techniques, Kinetics, Models, Chemical, Nucleic Acid Hybridization, Photochemistry, Time Factors, Ultraviolet Rays.

Abstract

Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc. Natl. Acad Sci U.SA. 1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block. The synthesis cycle uses a combinatorial approach attaching one specific base per cycle, thus as many as 100 cycles need to be run to make an array of 25-mers. Time needed for deprotection/activation of the growing oligo chain determines overall manufacturing time and consequently also cost. In this report we demonstrate the development of photoprotected posphoramidite monomers for light directed array synthesis with increasing sensitivity to the UV light used. If combined with maskless array synthesis, this technology allows for synthesis of arrays with >780,000 different 25-mer oligonucleotides in about one hour and allows for high flexibility in array design and reiterative redesign. The arrays synthesized show high quality and reproducibility in our standard hybridization based assay.

DOI: 10.1081/ncn-200059241
PubMed: 16248056

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Le document en format XML

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<term>Molecular Biology (methods)</term>
<term>Nucleic Acid Hybridization</term>
<term>Oligonucleotide Array Sequence Analysis (instrumentation)</term>
<term>Oligonucleotide Probes</term>
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<front><div type="abstract" xml:lang="en">Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc. Natl. Acad Sci U.SA. 1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block. The synthesis cycle uses a combinatorial approach attaching one specific base per cycle, thus as many as 100 cycles need to be run to make an array of 25-mers. Time needed for deprotection/activation of the growing oligo chain determines overall manufacturing time and consequently also cost. In this report we demonstrate the development of photoprotected posphoramidite monomers for light directed array synthesis with increasing sensitivity to the UV light used. If combined with maskless array synthesis, this technology allows for synthesis of arrays with >780,000 different 25-mer oligonucleotides in about one hour and allows for high flexibility in array design and reiterative redesign. The arrays synthesized show high quality and reproducibility in our standard hybridization based assay.</div>
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<Abstract><AbstractText>Light directed synthesis of high-density oligonucleotide microarrays is currently performed using either ortho-nitro-benzyl-type [MeNPOC] (Pease, A.C.; Solas, D.; Sullivan, E.J.; Cronin, T.M.; Holmes, C.P.; Fodor, S.P.A. Proc. Natl. Acad Sci U.SA. 1994, 91, 6333.) or ortho-nitrophenylethyl-type [NPPOC] (Hasan, A.; Stengele, K.P.; Giegrich, H.; Cornwell, P.; Isham, K.R.; Sachleben, R.A.; Pfleiderer, W.; Foote, R.S. Tetrahedron 1997, 53, 424Z) protecting groups as the 5'-O-carbonate ester of the phosphoramidite building block. The synthesis cycle uses a combinatorial approach attaching one specific base per cycle, thus as many as 100 cycles need to be run to make an array of 25-mers. Time needed for deprotection/activation of the growing oligo chain determines overall manufacturing time and consequently also cost. In this report we demonstrate the development of photoprotected posphoramidite monomers for light directed array synthesis with increasing sensitivity to the UV light used. If combined with maskless array synthesis, this technology allows for synthesis of arrays with >780,000 different 25-mer oligonucleotides in about one hour and allows for high flexibility in array design and reiterative redesign. The arrays synthesized show high quality and reproducibility in our standard hybridization based assay.</AbstractText>
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Recent highlights on photolithic oligonucleotide array in situ synthesis.

Recent highlights on photolithic oligonucleotide array in situ synthesis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki