MersV1, Ncbi, Curation, bibRecord, 002838

[An improved chemico-enzymatic method of synthesizing long DNA segments].

Identifieur interne : 002838 ( Ncbi/Curation ); précédent : 002837; suivant : 002839

[An improved chemico-enzymatic method of synthesizing long DNA segments].

Auteurs : M L Kobets ; M I Rivkin ; V S Bogachev ; V P Kumarev

Source :

Bioorganicheskaia khimiia [ 0132-3423 ] ; 1988.

RBID : pubmed:3382431

Descripteurs français

KwdFr :
- ADN (analyse), ADN (biosynthèse), ADN simple brin (analyse), ADN simple brin (biosynthèse), Clonage moléculaire, DNA ligases, DNA polymerase I, Gènes de synthèse, Humains, Interféron de type I (génétique), Polydésoxyribonucléotides (analyse), Polydésoxyribonucléotides (biosynthèse), Polynucleotide ligases, Électrophorèse sur gel de polyacrylamide.
MESH :
- analyse : ADN, ADN simple brin, Polydésoxyribonucléotides.
- biosynthèse : ADN, ADN simple brin, Polydésoxyribonucléotides.
- génétique : Interféron de type I.
- Clonage moléculaire, DNA ligases, DNA polymerase I, Gènes de synthèse, Humains, Polynucleotide ligases, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Cloning, Molecular, DNA (analysis), DNA (biosynthesis), DNA Ligases, DNA Polymerase I, DNA, Single-Stranded (analysis), DNA, Single-Stranded (biosynthesis), Electrophoresis, Polyacrylamide Gel, Genes, Synthetic, Humans, Interferon Type I (genetics), Polydeoxyribonucleotides (analysis), Polydeoxyribonucleotides (biosynthesis), Polynucleotide Ligases.
MESH :
- chemical , analysis : DNA, DNA, Single-Stranded, Polydeoxyribonucleotides.
- chemical , biosynthesis : DNA, DNA, Single-Stranded, Polydeoxyribonucleotides.
- chemical , genetics : Interferon Type I.
- Cloning, Molecular, DNA Ligases, DNA Polymerase I, Electrophoresis, Polyacrylamide Gel, Genes, Synthetic, Humans, Polynucleotide Ligases.

Abstract

A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.

PubMed: 3382431

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pubmed:3382431

Le document en format XML

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<author><name sortKey="Kobets, M L" sort="Kobets, M L" uniqKey="Kobets M" first="M L" last="Kobets">M L Kobets</name>
</author>
<author><name sortKey="Rivkin, M I" sort="Rivkin, M I" uniqKey="Rivkin M" first="M I" last="Rivkin">M I Rivkin</name>
</author>
<author><name sortKey="Bogachev, V S" sort="Bogachev, V S" uniqKey="Bogachev V" first="V S" last="Bogachev">V S Bogachev</name>
</author>
<author><name sortKey="Kumarev, V P" sort="Kumarev, V P" uniqKey="Kumarev V" first="V P" last="Kumarev">V P Kumarev</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[An improved chemico-enzymatic method of synthesizing long DNA segments].</title>
<author><name sortKey="Kobets, M L" sort="Kobets, M L" uniqKey="Kobets M" first="M L" last="Kobets">M L Kobets</name>
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<author><name sortKey="Rivkin, M I" sort="Rivkin, M I" uniqKey="Rivkin M" first="M I" last="Rivkin">M I Rivkin</name>
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<author><name sortKey="Bogachev, V S" sort="Bogachev, V S" uniqKey="Bogachev V" first="V S" last="Bogachev">V S Bogachev</name>
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<series><title level="j">Bioorganicheskaia khimiia</title>
<idno type="ISSN">0132-3423</idno>
<imprint><date when="1988" type="published">1988</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term>
<term>DNA (analysis)</term>
<term>DNA (biosynthesis)</term>
<term>DNA Ligases</term>
<term>DNA Polymerase I</term>
<term>DNA, Single-Stranded (analysis)</term>
<term>DNA, Single-Stranded (biosynthesis)</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Genes, Synthetic</term>
<term>Humans</term>
<term>Interferon Type I (genetics)</term>
<term>Polydeoxyribonucleotides (analysis)</term>
<term>Polydeoxyribonucleotides (biosynthesis)</term>
<term>Polynucleotide Ligases</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>ADN (biosynthèse)</term>
<term>ADN simple brin (analyse)</term>
<term>ADN simple brin (biosynthèse)</term>
<term>Clonage moléculaire</term>
<term>DNA ligases</term>
<term>DNA polymerase I</term>
<term>Gènes de synthèse</term>
<term>Humains</term>
<term>Interféron de type I (génétique)</term>
<term>Polydésoxyribonucléotides (analyse)</term>
<term>Polydésoxyribonucléotides (biosynthèse)</term>
<term>Polynucleotide ligases</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
<term>DNA, Single-Stranded</term>
<term>Polydeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA</term>
<term>DNA, Single-Stranded</term>
<term>Polydeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferon Type I</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
<term>ADN simple brin</term>
<term>Polydésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN</term>
<term>ADN simple brin</term>
<term>Polydésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Interféron de type I</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term>
<term>DNA Ligases</term>
<term>DNA Polymerase I</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Genes, Synthetic</term>
<term>Humans</term>
<term>Polynucleotide Ligases</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term>
<term>DNA ligases</term>
<term>DNA polymerase I</term>
<term>Gènes de synthèse</term>
<term>Humains</term>
<term>Polynucleotide ligases</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.</div>
</front>
</TEI>
</record>

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[An improved chemico-enzymatic method of synthesizing long DNA segments].

[An improved chemico-enzymatic method of synthesizing long DNA segments].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki