[An improved chemico-enzymatic method of synthesizing long DNA segments].
Identifieur interne : 002893 ( PubMed/Checkpoint ); précédent : 002892; suivant : 002894[An improved chemico-enzymatic method of synthesizing long DNA segments].
Auteurs : M L Kobets ; M I Rivkin ; V S Bogachev ; V P KumarevSource :
- Bioorganicheskaia khimiia [ 0132-3423 ] ; 1988.
Descripteurs français
- KwdFr :
- ADN (analyse), ADN (biosynthèse), ADN simple brin (analyse), ADN simple brin (biosynthèse), Clonage moléculaire, DNA ligases, DNA polymerase I, Gènes de synthèse, Humains, Interféron de type I (génétique), Polydésoxyribonucléotides (analyse), Polydésoxyribonucléotides (biosynthèse), Polynucleotide ligases, Électrophorèse sur gel de polyacrylamide.
- MESH :
- analyse : ADN, ADN simple brin, Polydésoxyribonucléotides.
- biosynthèse : ADN, ADN simple brin, Polydésoxyribonucléotides.
- génétique : Interféron de type I.
- Clonage moléculaire, DNA ligases, DNA polymerase I, Gènes de synthèse, Humains, Polynucleotide ligases, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Cloning, Molecular, DNA (analysis), DNA (biosynthesis), DNA Ligases, DNA Polymerase I, DNA, Single-Stranded (analysis), DNA, Single-Stranded (biosynthesis), Electrophoresis, Polyacrylamide Gel, Genes, Synthetic, Humans, Interferon Type I (genetics), Polydeoxyribonucleotides (analysis), Polydeoxyribonucleotides (biosynthesis), Polynucleotide Ligases.
- MESH :
- chemical , analysis : DNA, DNA, Single-Stranded, Polydeoxyribonucleotides.
- chemical , biosynthesis : DNA, DNA, Single-Stranded, Polydeoxyribonucleotides.
- chemical , genetics : Interferon Type I.
- Cloning, Molecular, DNA Ligases, DNA Polymerase I, Electrophoresis, Polyacrylamide Gel, Genes, Synthetic, Humans, Polynucleotide Ligases.
Abstract
A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.
PubMed: 3382431
Affiliations:
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pubmed:3382431Le document en format XML
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<author><name sortKey="Kobets, M L" sort="Kobets, M L" uniqKey="Kobets M" first="M L" last="Kobets">M L Kobets</name>
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<author><name sortKey="Rivkin, M I" sort="Rivkin, M I" uniqKey="Rivkin M" first="M I" last="Rivkin">M I Rivkin</name>
</author>
<author><name sortKey="Bogachev, V S" sort="Bogachev, V S" uniqKey="Bogachev V" first="V S" last="Bogachev">V S Bogachev</name>
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<author><name sortKey="Kumarev, V P" sort="Kumarev, V P" uniqKey="Kumarev V" first="V P" last="Kumarev">V P Kumarev</name>
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<term>DNA (analysis)</term>
<term>DNA (biosynthesis)</term>
<term>DNA Ligases</term>
<term>DNA Polymerase I</term>
<term>DNA, Single-Stranded (analysis)</term>
<term>DNA, Single-Stranded (biosynthesis)</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Genes, Synthetic</term>
<term>Humans</term>
<term>Interferon Type I (genetics)</term>
<term>Polydeoxyribonucleotides (analysis)</term>
<term>Polydeoxyribonucleotides (biosynthesis)</term>
<term>Polynucleotide Ligases</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>ADN (biosynthèse)</term>
<term>ADN simple brin (analyse)</term>
<term>ADN simple brin (biosynthèse)</term>
<term>Clonage moléculaire</term>
<term>DNA ligases</term>
<term>DNA polymerase I</term>
<term>Gènes de synthèse</term>
<term>Humains</term>
<term>Interféron de type I (génétique)</term>
<term>Polydésoxyribonucléotides (analyse)</term>
<term>Polydésoxyribonucléotides (biosynthèse)</term>
<term>Polynucleotide ligases</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
<term>DNA, Single-Stranded</term>
<term>Polydeoxyribonucleotides</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA</term>
<term>DNA, Single-Stranded</term>
<term>Polydeoxyribonucleotides</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferon Type I</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
<term>ADN simple brin</term>
<term>Polydésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN</term>
<term>ADN simple brin</term>
<term>Polydésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Interféron de type I</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term>
<term>DNA Ligases</term>
<term>DNA Polymerase I</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Genes, Synthetic</term>
<term>Humans</term>
<term>Polynucleotide Ligases</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term>
<term>DNA ligases</term>
<term>DNA polymerase I</term>
<term>Gènes de synthèse</term>
<term>Humains</term>
<term>Polynucleotide ligases</term>
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<front><div type="abstract" xml:lang="en">A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.</div>
</front>
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<Month>07</Month>
<Day>27</Day>
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<DateRevised><Year>2006</Year>
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<Issue>1</Issue>
<PubDate><Year>1988</Year>
<Month>Jan</Month>
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<Title>Bioorganicheskaia khimiia</Title>
<ISOAbbreviation>Bioorg. Khim.</ISOAbbreviation>
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<ArticleTitle>[An improved chemico-enzymatic method of synthesizing long DNA segments].</ArticleTitle>
<Pagination><MedlinePgn>43-7</MedlinePgn>
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<Abstract><AbstractText>A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.</AbstractText>
</Abstract>
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<VernacularTitle>Usovershenstvovannyĭ khimiko-fermentativnyĭ metod sinteza protiazhennykh fragmentov DNK.</VernacularTitle>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
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<name sortKey="Kobets, M L" sort="Kobets, M L" uniqKey="Kobets M" first="M L" last="Kobets">M L Kobets</name>
<name sortKey="Kumarev, V P" sort="Kumarev, V P" uniqKey="Kumarev V" first="V P" last="Kumarev">V P Kumarev</name>
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