MersV1, PubMed, Corpus, bibRecord, 002A69

[An improved chemico-enzymatic method of synthesizing long DNA segments].

Identifieur interne : 002A69 ( PubMed/Corpus ); précédent : 002A68; suivant : 002A70

[An improved chemico-enzymatic method of synthesizing long DNA segments].

Auteurs : M L Kobets ; M I Rivkin ; V S Bogachev ; V P Kumarev

Source :

Bioorganicheskaia khimiia [ 0132-3423 ] ; 1988.

RBID : pubmed:3382431

English descriptors

KwdEn :
- Cloning, Molecular, DNA (analysis), DNA (biosynthesis), DNA Ligases, DNA Polymerase I, DNA, Single-Stranded (analysis), DNA, Single-Stranded (biosynthesis), Electrophoresis, Polyacrylamide Gel, Genes, Synthetic, Humans, Interferon Type I (genetics), Polydeoxyribonucleotides (analysis), Polydeoxyribonucleotides (biosynthesis), Polynucleotide Ligases.
MESH :
- chemical , analysis : DNA, DNA, Single-Stranded, Polydeoxyribonucleotides.
- chemical , biosynthesis : DNA, DNA, Single-Stranded, Polydeoxyribonucleotides.
- chemical , genetics : Interferon Type I.
- Cloning, Molecular, DNA Ligases, DNA Polymerase I, Electrophoresis, Polyacrylamide Gel, Genes, Synthetic, Humans, Polynucleotide Ligases.

Abstract

A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.

PubMed: 3382431

Links to Exploration step

pubmed:3382431

Le document en format XML

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<author><name sortKey="Kobets, M L" sort="Kobets, M L" uniqKey="Kobets M" first="M L" last="Kobets">M L Kobets</name>
</author>
<author><name sortKey="Rivkin, M I" sort="Rivkin, M I" uniqKey="Rivkin M" first="M I" last="Rivkin">M I Rivkin</name>
</author>
<author><name sortKey="Bogachev, V S" sort="Bogachev, V S" uniqKey="Bogachev V" first="V S" last="Bogachev">V S Bogachev</name>
</author>
<author><name sortKey="Kumarev, V P" sort="Kumarev, V P" uniqKey="Kumarev V" first="V P" last="Kumarev">V P Kumarev</name>
</author>
</titleStmt>
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<date when="1988">1988</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[An improved chemico-enzymatic method of synthesizing long DNA segments].</title>
<author><name sortKey="Kobets, M L" sort="Kobets, M L" uniqKey="Kobets M" first="M L" last="Kobets">M L Kobets</name>
</author>
<author><name sortKey="Rivkin, M I" sort="Rivkin, M I" uniqKey="Rivkin M" first="M I" last="Rivkin">M I Rivkin</name>
</author>
<author><name sortKey="Bogachev, V S" sort="Bogachev, V S" uniqKey="Bogachev V" first="V S" last="Bogachev">V S Bogachev</name>
</author>
<author><name sortKey="Kumarev, V P" sort="Kumarev, V P" uniqKey="Kumarev V" first="V P" last="Kumarev">V P Kumarev</name>
</author>
</analytic>
<series><title level="j">Bioorganicheskaia khimiia</title>
<idno type="ISSN">0132-3423</idno>
<imprint><date when="1988" type="published">1988</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term>
<term>DNA (analysis)</term>
<term>DNA (biosynthesis)</term>
<term>DNA Ligases</term>
<term>DNA Polymerase I</term>
<term>DNA, Single-Stranded (analysis)</term>
<term>DNA, Single-Stranded (biosynthesis)</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Genes, Synthetic</term>
<term>Humans</term>
<term>Interferon Type I (genetics)</term>
<term>Polydeoxyribonucleotides (analysis)</term>
<term>Polydeoxyribonucleotides (biosynthesis)</term>
<term>Polynucleotide Ligases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
<term>DNA, Single-Stranded</term>
<term>Polydeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA</term>
<term>DNA, Single-Stranded</term>
<term>Polydeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferon Type I</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term>
<term>DNA Ligases</term>
<term>DNA Polymerase I</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Genes, Synthetic</term>
<term>Humans</term>
<term>Polynucleotide Ligases</term>
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<front><div type="abstract" xml:lang="en">A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">3382431</PMID>
<DateCompleted><Year>1988</Year>
<Month>07</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0132-3423</ISSN>
<JournalIssue CitedMedium="Print"><Volume>14</Volume>
<Issue>1</Issue>
<PubDate><Year>1988</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Bioorganicheskaia khimiia</Title>
<ISOAbbreviation>Bioorg. Khim.</ISOAbbreviation>
</Journal>
<ArticleTitle>[An improved chemico-enzymatic method of synthesizing long DNA segments].</ArticleTitle>
<Pagination><MedlinePgn>43-7</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A simple and economy method of the biochemical assembling of long double-stranded DNA segments is described. A single-stranded polydeoxynucleotide 122 bases long representing a fragment of synthetic gene of human beta-interferon was assembled from three synthetic fragments 36 (two) and 50 bases long on four complementary 12-mers as templates. This single-stranded polynucleotide was converted, in the presence of DNA polymerase 1 and a 12-meric primer, in to the full-length double-stranded DNA (the beta-interferon gene segment). It was cloned into an E. coli plasmid vector pBR322 and its sequence confirmed.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kobets</LastName>
<ForeName>M L</ForeName>
<Initials>ML</Initials>
</Author>
<Author ValidYN="Y"><LastName>Rivkin</LastName>
<ForeName>M I</ForeName>
<Initials>MI</Initials>
</Author>
<Author ValidYN="Y"><LastName>Bogachev</LastName>
<ForeName>V S</ForeName>
<Initials>VS</Initials>
</Author>
<Author ValidYN="Y"><LastName>Kumarev</LastName>
<ForeName>V P</ForeName>
<Initials>VP</Initials>
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<Language>rus</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<VernacularTitle>Usovershenstvovannyĭ khimiko-fermentativnyĭ metod sinteza protiazhennykh fragmentov DNK.</VernacularTitle>
</Article>
<MedlineJournalInfo><Country>Russia (Federation)</Country>
<MedlineTA>Bioorg Khim</MedlineTA>
<NlmUniqueID>7804941</NlmUniqueID>
<ISSNLinking>0132-3423</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004277">DNA, Single-Stranded</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007370">Interferon Type I</NameOfSubstance>
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<Chemical><RegistryNumber>EC 2.7.7.-</RegistryNumber>
<NameOfSubstance UI="D004256">DNA Polymerase I</NameOfSubstance>
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<Chemical><RegistryNumber>EC 6.5.1.-</RegistryNumber>
<NameOfSubstance UI="D011088">DNA Ligases</NameOfSubstance>
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<Chemical><RegistryNumber>EC 6.5.1.-</RegistryNumber>
<NameOfSubstance UI="D011118">Polynucleotide Ligases</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
<QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName>
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<MeshHeading><DescriptorName UI="D011088" MajorTopicYN="Y">DNA Ligases</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004256" MajorTopicYN="Y">DNA Polymerase I</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004277" MajorTopicYN="N">DNA, Single-Stranded</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005813" MajorTopicYN="Y">Genes, Synthetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007370" MajorTopicYN="N">Interferon Type I</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011089" MajorTopicYN="N">Polydeoxyribonucleotides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
<QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011118" MajorTopicYN="Y">Polynucleotide Ligases</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year>
<Month>1</Month>
<Day>1</Day>
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</record>

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[An improved chemico-enzymatic method of synthesizing long DNA segments].

[An improved chemico-enzymatic method of synthesizing long DNA segments].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki