Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.
Identifieur interne : 000237 ( Ncbi/Curation ); précédent : 000236; suivant : 000238Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.
Auteurs : J. Lisziewicz [États-Unis] ; D. Sun ; M. Klotman ; S. Agrawal ; P. Zamecnik ; R. GalloSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1992.
Descripteurs français
- KwdFr :
- Cellules cultivées, Données de séquences moléculaires, Facteurs temps, Humains, Mutation, Oligonucléotides antisens (), Oligonucléotides antisens (pharmacologie), Réplication virale (), Survie cellulaire (), Séquence nucléotidique, Techniques in vitro, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (croissance et développement), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique).
- MESH :
- croissance et développement : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- génétique : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- pharmacologie : Oligonucléotides antisens.
- Cellules cultivées, Données de séquences moléculaires, Facteurs temps, Humains, Mutation, Oligonucléotides antisens, Réplication virale, Survie cellulaire, Séquence nucléotidique, Techniques in vitro.
English descriptors
- KwdEn :
- Base Sequence, Cell Survival (drug effects), Cells, Cultured, HIV-1 (genetics), HIV-1 (growth & development), Humans, In Vitro Techniques, Molecular Sequence Data, Mutation, Oligonucleotides, Antisense (chemistry), Oligonucleotides, Antisense (pharmacology), Time Factors, Virus Replication (drug effects).
- MESH :
- chemical , chemistry : Oligonucleotides, Antisense.
- drug effects : Cell Survival, Virus Replication.
- genetics : HIV-1.
- growth & development : HIV-1.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Base Sequence, Cells, Cultured, Humans, In Vitro Techniques, Molecular Sequence Data, Mutation, Time Factors.
Abstract
We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication of the virus in a sequence-specific manner at 1 microM concentration. Variations in antiviral activity were seen among the different oligonucleotides, revealing an effect of target selection. Mismatched or random oligonucleotide phosphorothioates delayed, but did not completely inhibit, HIV-1 replication. In the case of inhibition by a splice-acceptor-site antisense oligodeoxynucleotide, a break-through phenomenon occurred after 25 days of treatment, suggesting the development of an "escape mutant." This result did not occur when the inhibitory oligodeoxynucleotides were complementary to the primary-sequence areas of the rev-responsive element and rev-1 genes. Sequential treatment of HIV-1-infected cells with a combination of different antisense oligonucleotides, each administered once, also prevented the development of escape mutants. Our results suggest that chemotherapy based on specifically targeted antisense-oligonucleotide phosphorothioates may be an effective method for reducing the viral burden in HIV-1-infected individuals at clinically achievable oligonucleotide concentrations.
DOI: 10.1073/pnas.89.23.11209
PubMed: 1454800
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002936
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002936
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002794
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000237
Links to Exploration step
pubmed:1454800Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.</title><author><name sortKey="Lisziewicz, J" sort="Lisziewicz, J" uniqKey="Lisziewicz J" first="J" last="Lisziewicz">J. Lisziewicz</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Sun, D" sort="Sun, D" uniqKey="Sun D" first="D" last="Sun">D. Sun</name></author><author><name sortKey="Klotman, M" sort="Klotman, M" uniqKey="Klotman M" first="M" last="Klotman">M. Klotman</name></author><author><name sortKey="Agrawal, S" sort="Agrawal, S" uniqKey="Agrawal S" first="S" last="Agrawal">S. Agrawal</name></author><author><name sortKey="Zamecnik, P" sort="Zamecnik, P" uniqKey="Zamecnik P" first="P" last="Zamecnik">P. Zamecnik</name></author><author><name sortKey="Gallo, R" sort="Gallo, R" uniqKey="Gallo R" first="R" last="Gallo">R. Gallo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1454800</idno><idno type="pmid">1454800</idno><idno type="doi">10.1073/pnas.89.23.11209</idno><idno type="wicri:Area/PubMed/Corpus">002936</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002936</idno><idno type="wicri:Area/PubMed/Curation">002936</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002936</idno><idno type="wicri:Area/PubMed/Checkpoint">002794</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002794</idno><idno type="wicri:Area/Ncbi/Merge">000237</idno><idno type="wicri:Area/Ncbi/Curation">000237</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.</title><author><name sortKey="Lisziewicz, J" sort="Lisziewicz, J" uniqKey="Lisziewicz J" first="J" last="Lisziewicz">J. Lisziewicz</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Sun, D" sort="Sun, D" uniqKey="Sun D" first="D" last="Sun">D. Sun</name></author><author><name sortKey="Klotman, M" sort="Klotman, M" uniqKey="Klotman M" first="M" last="Klotman">M. Klotman</name></author><author><name sortKey="Agrawal, S" sort="Agrawal, S" uniqKey="Agrawal S" first="S" last="Agrawal">S. Agrawal</name></author><author><name sortKey="Zamecnik, P" sort="Zamecnik, P" uniqKey="Zamecnik P" first="P" last="Zamecnik">P. Zamecnik</name></author><author><name sortKey="Gallo, R" sort="Gallo, R" uniqKey="Gallo R" first="R" last="Gallo">R. Gallo</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Cell Survival (drug effects)</term><term>Cells, Cultured</term><term>HIV-1 (genetics)</term><term>HIV-1 (growth & development)</term><term>Humans</term><term>In Vitro Techniques</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Oligonucleotides, Antisense (chemistry)</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>Time Factors</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Facteurs temps</term><term>Humains</term><term>Mutation</term><term>Oligonucléotides antisens ()</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Réplication virale ()</term><term>Survie cellulaire ()</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (croissance et développement)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cells, Cultured</term><term>Humans</term><term>In Vitro Techniques</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Facteurs temps</term><term>Humains</term><term>Mutation</term><term>Oligonucléotides antisens</term><term>Réplication virale</term><term>Survie cellulaire</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication of the virus in a sequence-specific manner at 1 microM concentration. Variations in antiviral activity were seen among the different oligonucleotides, revealing an effect of target selection. Mismatched or random oligonucleotide phosphorothioates delayed, but did not completely inhibit, HIV-1 replication. In the case of inhibition by a splice-acceptor-site antisense oligodeoxynucleotide, a break-through phenomenon occurred after 25 days of treatment, suggesting the development of an "escape mutant." This result did not occur when the inhibitory oligodeoxynucleotides were complementary to the primary-sequence areas of the rev-responsive element and rev-1 genes. Sequential treatment of HIV-1-infected cells with a combination of different antisense oligonucleotides, each administered once, also prevented the development of escape mutants. Our results suggest that chemotherapy based on specifically targeted antisense-oligonucleotide phosphorothioates may be an effective method for reducing the viral burden in HIV-1-infected individuals at clinically achievable oligonucleotide concentrations.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000237 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000237 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= pubmed:1454800
|texte= Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:1454800" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |