A-hydroxyphosphonate oligonucleotides: a promising DNA type?
Identifieur interne : 000238 ( Ncbi/Curation ); précédent : 000237; suivant : 000239A-hydroxyphosphonate oligonucleotides: a promising DNA type?
Auteurs : Sárka Králíková [République tchèque] ; Milos Budesínsk ; Ivan RosenbergSource :
- Nucleosides, nucleotides & nucleic acids [ 1525-7770 ]
Descripteurs français
- KwdFr :
- MESH :
- analogues et dérivés : Thymidine.
- synthèse chimique : Oligodésoxyribonucléotides.
- ADN, Conformation d'acide nucléique, Indicateurs et réactifs, Oligodésoxyribonucléotides, Phosphonates, Structure moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Thymidine.
- chemical , chemical synthesis : Oligodeoxyribonucleotides.
- chemical , chemistry : DNA, Oligodeoxyribonucleotides.
- chemical : Indicators and Reagents, Organophosphonates.
- Molecular Structure, Nucleic Acid Conformation.
Abstract
The synthesis of monomers (S)-1, (R)-1 and 2 derived from (5'S)-, (5'R)-2'-deoxythymidine-5'-C-phosphonic acids and 2',5'-dideoxythymidine-5'-C-phosphonic acids was elaborated. The protection of the 5'-hydroxyl by the methoxycarbonyl group was a key step of the synthesis. Prepared monomers were used for the solid-phase assembly of several types oligothymidylate 15-mers (S)-3, (S)-4, (S)-5, (R)-4 and (R)-5 containing the chiral 3'-O-P-CH(OH)-5'' internucleotide linkage. Their hybridization properties with dA15 and rA15 were studied as well as their resistance against nuclease cleavage.
DOI: 10.1081/NCN-120022736
PubMed: 14565344
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pubmed:14565344Le document en format XML
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type?</title><author><name sortKey="Kralikova, Sarka" sort="Kralikova, Sarka" uniqKey="Kralikova S" first="Sárka" last="Králíková">Sárka Králíková</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Prague, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation></author><author><name sortKey="Budesinsk, Milos" sort="Budesinsk, Milos" uniqKey="Budesinsk M" first="Milos" last="Budesínsk">Milos Budesínsk</name></author><author><name sortKey="Rosenberg, Ivan" sort="Rosenberg, Ivan" uniqKey="Rosenberg I" first="Ivan" last="Rosenberg">Ivan Rosenberg</name></author></analytic><series><title level="j">Nucleosides, nucleotides & nucleic 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type="abstract" xml:lang="en">The synthesis of monomers (S)-1, (R)-1 and 2 derived from (5'S)-, (5'R)-2'-deoxythymidine-5'-C-phosphonic acids and 2',5'-dideoxythymidine-5'-C-phosphonic acids was elaborated. The protection of the 5'-hydroxyl by the methoxycarbonyl group was a key step of the synthesis. Prepared monomers were used for the solid-phase assembly of several types oligothymidylate 15-mers (S)-3, (S)-4, (S)-5, (R)-4 and (R)-5 containing the chiral 3'-O-P-CH(OH)-5'' internucleotide linkage. Their hybridization properties with dA15 and rA15 were studied as well as their resistance against nuclease cleavage.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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