Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.
Identifieur interne : 000237 ( Ncbi/Merge ); précédent : 000236; suivant : 000238Specific inhibition of human immunodeficiency virus type 1 replication by antisense oligonucleotides: an in vitro model for treatment.
Auteurs : J. Lisziewicz [États-Unis] ; D. Sun ; M. Klotman ; S. Agrawal ; P. Zamecnik ; R. GalloSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1992.
Descripteurs français
- KwdFr :
- Cellules cultivées, Données de séquences moléculaires, Facteurs temps, Humains, Mutation, Oligonucléotides antisens (), Oligonucléotides antisens (pharmacologie), Réplication virale (), Survie cellulaire (), Séquence nucléotidique, Techniques in vitro, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (croissance et développement), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique).
- MESH :
- croissance et développement : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- génétique : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- pharmacologie : Oligonucléotides antisens.
- Cellules cultivées, Données de séquences moléculaires, Facteurs temps, Humains, Mutation, Oligonucléotides antisens, Réplication virale, Survie cellulaire, Séquence nucléotidique, Techniques in vitro.
English descriptors
- KwdEn :
- Base Sequence, Cell Survival (drug effects), Cells, Cultured, HIV-1 (genetics), HIV-1 (growth & development), Humans, In Vitro Techniques, Molecular Sequence Data, Mutation, Oligonucleotides, Antisense (chemistry), Oligonucleotides, Antisense (pharmacology), Time Factors, Virus Replication (drug effects).
- MESH :
- chemical , chemistry : Oligonucleotides, Antisense.
- drug effects : Cell Survival, Virus Replication.
- genetics : HIV-1.
- growth & development : HIV-1.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Base Sequence, Cells, Cultured, Humans, In Vitro Techniques, Molecular Sequence Data, Mutation, Time Factors.
Abstract
We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication of the virus in a sequence-specific manner at 1 microM concentration. Variations in antiviral activity were seen among the different oligonucleotides, revealing an effect of target selection. Mismatched or random oligonucleotide phosphorothioates delayed, but did not completely inhibit, HIV-1 replication. In the case of inhibition by a splice-acceptor-site antisense oligodeoxynucleotide, a break-through phenomenon occurred after 25 days of treatment, suggesting the development of an "escape mutant." This result did not occur when the inhibitory oligodeoxynucleotides were complementary to the primary-sequence areas of the rev-responsive element and rev-1 genes. Sequential treatment of HIV-1-infected cells with a combination of different antisense oligonucleotides, each administered once, also prevented the development of escape mutants. Our results suggest that chemotherapy based on specifically targeted antisense-oligonucleotide phosphorothioates may be an effective method for reducing the viral burden in HIV-1-infected individuals at clinically achievable oligonucleotide concentrations.
DOI: 10.1073/pnas.89.23.11209
PubMed: 1454800
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002936
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pubmed:1454800Le document en format XML
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<front><div type="abstract" xml:lang="en">We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication of the virus in a sequence-specific manner at 1 microM concentration. Variations in antiviral activity were seen among the different oligonucleotides, revealing an effect of target selection. Mismatched or random oligonucleotide phosphorothioates delayed, but did not completely inhibit, HIV-1 replication. In the case of inhibition by a splice-acceptor-site antisense oligodeoxynucleotide, a break-through phenomenon occurred after 25 days of treatment, suggesting the development of an "escape mutant." This result did not occur when the inhibitory oligodeoxynucleotides were complementary to the primary-sequence areas of the rev-responsive element and rev-1 genes. Sequential treatment of HIV-1-infected cells with a combination of different antisense oligonucleotides, each administered once, also prevented the development of escape mutants. Our results suggest that chemotherapy based on specifically targeted antisense-oligonucleotide phosphorothioates may be an effective method for reducing the viral burden in HIV-1-infected individuals at clinically achievable oligonucleotide concentrations.</div>
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<Abstract><AbstractText>We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment. Five oligonucleotide phosphorothioates (28-mers), complementary to different regions of HIV-1 RNA, blocked replication of the virus in a sequence-specific manner at 1 microM concentration. Variations in antiviral activity were seen among the different oligonucleotides, revealing an effect of target selection. Mismatched or random oligonucleotide phosphorothioates delayed, but did not completely inhibit, HIV-1 replication. In the case of inhibition by a splice-acceptor-site antisense oligodeoxynucleotide, a break-through phenomenon occurred after 25 days of treatment, suggesting the development of an "escape mutant." This result did not occur when the inhibitory oligodeoxynucleotides were complementary to the primary-sequence areas of the rev-responsive element and rev-1 genes. Sequential treatment of HIV-1-infected cells with a combination of different antisense oligonucleotides, each administered once, also prevented the development of escape mutants. Our results suggest that chemotherapy based on specifically targeted antisense-oligonucleotide phosphorothioates may be an effective method for reducing the viral burden in HIV-1-infected individuals at clinically achievable oligonucleotide concentrations.</AbstractText>
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<ArticleIdList><ArticleId IdType="pubmed">2225067</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1990 Jun;87(12):4571-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2191294</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1990 Feb 23;60(4):675-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2406030</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Natl Cancer Inst. 1987 Apr;78(4):663-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2435942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1989 Jun;86(11):4244-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2471199</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1985 Jan 24-30;313(6000):277-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2578615</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1989 Oct;86(20):7790-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2682627</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1985 Jul 5;229(4708):69-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2990040</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1986 Jun;83(12):4143-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3012555</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1988 Aug;85(15):5507-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3041414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1988 Oct;85(19):7079-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3174622</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1988 Oct;85(20):7448-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3174646</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biochem Biophys Methods. 1986 Sep;13(2):97-102</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3772027</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1984 May 4;224(4648):497-500</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6200935</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gene. 1988 Dec 10;72(1-2):343-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3243433</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biotechnol. 1992 May;10(5):152-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1368317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1992 Jan;66(1):600-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1727504</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):758-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1731351</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1991 Apr 19;65(2):241-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2015625</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1991 Jun 25;19(12):3359-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2062653</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1990 Nov 25;265(33):20172-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2173699</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1990 Jun 29;61(7):1271-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2364429</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
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