Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity.
Identifieur interne : 002320 ( Ncbi/Checkpoint ); précédent : 002319; suivant : 002321Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity.
Auteurs : Bryan A. Johnson [États-Unis] ; Adam Hage [États-Unis] ; Birte Kalveram [États-Unis] ; Megan Mears [États-Unis] ; Jessica A. Plante [États-Unis] ; Sergio E. Rodriguez [États-Unis] ; Zhixia Ding [États-Unis] ; Xuemei Luo [États-Unis] ; Dennis Bente [États-Unis] ; Shelton S. Bradrick [États-Unis] ; Alexander N. Freiberg [États-Unis] ; Vsevolod Popov [États-Unis] ; Ricardo Rajsbaum [États-Unis] ; Shannan Rossi [États-Unis] ; William K. Russell [États-Unis] ; Vineet D. Menachery [États-Unis]Source :
- Journal of virology [ 1098-5514 ] ; 2019.
Abstract
Enteric viruses exploit bacterial components, including lipopolysaccharides (LPS) and peptidoglycan (PG), to facilitate infection in humans. Because of their origin in the bat enteric system, we wondered if severe acute respiratory syndrome coronavirus (SARS-CoV) or Middle East respiratory syndrome CoV (MERS-CoV) also use bacterial components to modulate infectivity. To test this question, we incubated CoVs with LPS and PG and evaluated infectivity, finding no change following LPS treatment. However, PG from Bacillus subtilis reduced infection >10,000-fold, while PG from other bacterial species failed to recapitulate this. Treatment with an alcohol solvent transferred inhibitory activity to the wash, and mass spectrometry revealed surfactin, a cyclic lipopeptide antibiotic, as the inhibitory compound. This antibiotic had robust dose- and temperature-dependent inhibition of CoV infectivity. Mechanistic studies indicated that surfactin disrupts CoV virion integrity, and surfactin treatment of the virus inoculum ablated infection in vivo Finally, similar cyclic lipopeptides had no effect on CoV infectivity, and the inhibitory effect of surfactin extended broadly to enveloped viruses, including influenza, Ebola, Zika, Nipah, chikungunya, Una, Mayaro, Dugbe, and Crimean-Congo hemorrhagic fever viruses. Overall, our results indicate that peptidoglycan-associated surfactin has broad viricidal activity and suggest that bacteria by-products may negatively modulate virus infection.IMPORTANCE In this article, we consider a role for bacteria in shaping coronavirus infection. Taking cues from studies of enteric viruses, we initially investigated how bacterial surface components might improve CoV infection. Instead, we found that peptidoglycan-associated surfactin is a potent viricidal compound that disrupts virion integrity with broad activity against enveloped viruses. Our results indicate that interactions with commensal bacterial may improve or disrupt viral infections, highlighting the importance of understanding these microbial interactions and their implications for viral pathogenesis and treatment.
DOI: 10.1128/JVI.01282-19
PubMed: 31462558
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000440
- to stream PubMed, to step Curation: 000440
- to stream PubMed, to step Checkpoint: 000433
- to stream Ncbi, to step Merge: 002320
- to stream Ncbi, to step Curation: 002320
Links to Exploration step
pubmed:31462558Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity.</title>
<author><name sortKey="Johnson, Bryan A" sort="Johnson, Bryan A" uniqKey="Johnson B" first="Bryan A" last="Johnson">Bryan A. Johnson</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hage, Adam" sort="Hage, Adam" uniqKey="Hage A" first="Adam" last="Hage">Adam Hage</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kalveram, Birte" sort="Kalveram, Birte" uniqKey="Kalveram B" first="Birte" last="Kalveram">Birte Kalveram</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mears, Megan" sort="Mears, Megan" uniqKey="Mears M" first="Megan" last="Mears">Megan Mears</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name>
<affiliation wicri:level="2"><nlm:affiliation>World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rodriguez, Sergio E" sort="Rodriguez, Sergio E" uniqKey="Rodriguez S" first="Sergio E" last="Rodriguez">Sergio E. Rodriguez</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ding, Zhixia" sort="Ding, Zhixia" uniqKey="Ding Z" first="Zhixia" last="Ding">Zhixia Ding</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Luo, Xuemei" sort="Luo, Xuemei" uniqKey="Luo X" first="Xuemei" last="Luo">Xuemei Luo</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bente, Dennis" sort="Bente, Dennis" uniqKey="Bente D" first="Dennis" last="Bente">Dennis Bente</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bradrick, Shelton S" sort="Bradrick, Shelton S" uniqKey="Bradrick S" first="Shelton S" last="Bradrick">Shelton S. Bradrick</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Freiberg, Alexander N" sort="Freiberg, Alexander N" uniqKey="Freiberg A" first="Alexander N" last="Freiberg">Alexander N. Freiberg</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Popov, Vsevolod" sort="Popov, Vsevolod" uniqKey="Popov V" first="Vsevolod" last="Popov">Vsevolod Popov</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rajsbaum, Ricardo" sort="Rajsbaum, Ricardo" uniqKey="Rajsbaum R" first="Ricardo" last="Rajsbaum">Ricardo Rajsbaum</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rossi, Shannan" sort="Rossi, Shannan" uniqKey="Rossi S" first="Shannan" last="Rossi">Shannan Rossi</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Russell, William K" sort="Russell, William K" uniqKey="Russell W" first="William K" last="Russell">William K. Russell</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA Vimenach@utmb.edu.</nlm:affiliation>
<country wicri:rule="url">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2019">2019</date>
<idno type="RBID">pubmed:31462558</idno>
<idno type="pmid">31462558</idno>
<idno type="doi">10.1128/JVI.01282-19</idno>
<idno type="wicri:Area/PubMed/Corpus">000440</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000440</idno>
<idno type="wicri:Area/PubMed/Curation">000440</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000440</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000433</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000433</idno>
<idno type="wicri:Area/Ncbi/Merge">002320</idno>
<idno type="wicri:Area/Ncbi/Curation">002320</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002320</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity.</title>
<author><name sortKey="Johnson, Bryan A" sort="Johnson, Bryan A" uniqKey="Johnson B" first="Bryan A" last="Johnson">Bryan A. Johnson</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hage, Adam" sort="Hage, Adam" uniqKey="Hage A" first="Adam" last="Hage">Adam Hage</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kalveram, Birte" sort="Kalveram, Birte" uniqKey="Kalveram B" first="Birte" last="Kalveram">Birte Kalveram</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mears, Megan" sort="Mears, Megan" uniqKey="Mears M" first="Megan" last="Mears">Megan Mears</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name>
<affiliation wicri:level="2"><nlm:affiliation>World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rodriguez, Sergio E" sort="Rodriguez, Sergio E" uniqKey="Rodriguez S" first="Sergio E" last="Rodriguez">Sergio E. Rodriguez</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ding, Zhixia" sort="Ding, Zhixia" uniqKey="Ding Z" first="Zhixia" last="Ding">Zhixia Ding</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Luo, Xuemei" sort="Luo, Xuemei" uniqKey="Luo X" first="Xuemei" last="Luo">Xuemei Luo</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bente, Dennis" sort="Bente, Dennis" uniqKey="Bente D" first="Dennis" last="Bente">Dennis Bente</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bradrick, Shelton S" sort="Bradrick, Shelton S" uniqKey="Bradrick S" first="Shelton S" last="Bradrick">Shelton S. Bradrick</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Freiberg, Alexander N" sort="Freiberg, Alexander N" uniqKey="Freiberg A" first="Alexander N" last="Freiberg">Alexander N. Freiberg</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Popov, Vsevolod" sort="Popov, Vsevolod" uniqKey="Popov V" first="Vsevolod" last="Popov">Vsevolod Popov</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rajsbaum, Ricardo" sort="Rajsbaum, Ricardo" uniqKey="Rajsbaum R" first="Ricardo" last="Rajsbaum">Ricardo Rajsbaum</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rossi, Shannan" sort="Rossi, Shannan" uniqKey="Rossi S" first="Shannan" last="Rossi">Shannan Rossi</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Russell, William K" sort="Russell, William K" uniqKey="Russell W" first="William K" last="Russell">William K. Russell</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA Vimenach@utmb.edu.</nlm:affiliation>
<country wicri:rule="url">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2019" type="published">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Enteric viruses exploit bacterial components, including lipopolysaccharides (LPS) and peptidoglycan (PG), to facilitate infection in humans. Because of their origin in the bat enteric system, we wondered if severe acute respiratory syndrome coronavirus (SARS-CoV) or Middle East respiratory syndrome CoV (MERS-CoV) also use bacterial components to modulate infectivity. To test this question, we incubated CoVs with LPS and PG and evaluated infectivity, finding no change following LPS treatment. However, PG from <i>Bacillus subtilis</i>
reduced infection >10,000-fold, while PG from other bacterial species failed to recapitulate this. Treatment with an alcohol solvent transferred inhibitory activity to the wash, and mass spectrometry revealed surfactin, a cyclic lipopeptide antibiotic, as the inhibitory compound. This antibiotic had robust dose- and temperature-dependent inhibition of CoV infectivity. Mechanistic studies indicated that surfactin disrupts CoV virion integrity, and surfactin treatment of the virus inoculum ablated infection <i>in vivo</i>
Finally, similar cyclic lipopeptides had no effect on CoV infectivity, and the inhibitory effect of surfactin extended broadly to enveloped viruses, including influenza, Ebola, Zika, Nipah, chikungunya, Una, Mayaro, Dugbe, and Crimean-Congo hemorrhagic fever viruses. Overall, our results indicate that peptidoglycan-associated surfactin has broad viricidal activity and suggest that bacteria by-products may negatively modulate virus infection.<b>IMPORTANCE</b>
In this article, we consider a role for bacteria in shaping coronavirus infection. Taking cues from studies of enteric viruses, we initially investigated how bacterial surface components might improve CoV infection. Instead, we found that peptidoglycan-associated surfactin is a potent viricidal compound that disrupts virion integrity with broad activity against enveloped viruses. Our results indicate that interactions with commensal bacterial may improve or disrupt viral infections, highlighting the importance of understanding these microbial interactions and their implications for viral pathogenesis and treatment.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Texas</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Texas"><name sortKey="Johnson, Bryan A" sort="Johnson, Bryan A" uniqKey="Johnson B" first="Bryan A" last="Johnson">Bryan A. Johnson</name>
</region>
<name sortKey="Bente, Dennis" sort="Bente, Dennis" uniqKey="Bente D" first="Dennis" last="Bente">Dennis Bente</name>
<name sortKey="Bradrick, Shelton S" sort="Bradrick, Shelton S" uniqKey="Bradrick S" first="Shelton S" last="Bradrick">Shelton S. Bradrick</name>
<name sortKey="Ding, Zhixia" sort="Ding, Zhixia" uniqKey="Ding Z" first="Zhixia" last="Ding">Zhixia Ding</name>
<name sortKey="Freiberg, Alexander N" sort="Freiberg, Alexander N" uniqKey="Freiberg A" first="Alexander N" last="Freiberg">Alexander N. Freiberg</name>
<name sortKey="Hage, Adam" sort="Hage, Adam" uniqKey="Hage A" first="Adam" last="Hage">Adam Hage</name>
<name sortKey="Kalveram, Birte" sort="Kalveram, Birte" uniqKey="Kalveram B" first="Birte" last="Kalveram">Birte Kalveram</name>
<name sortKey="Luo, Xuemei" sort="Luo, Xuemei" uniqKey="Luo X" first="Xuemei" last="Luo">Xuemei Luo</name>
<name sortKey="Mears, Megan" sort="Mears, Megan" uniqKey="Mears M" first="Megan" last="Mears">Megan Mears</name>
<name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name>
<name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name>
<name sortKey="Popov, Vsevolod" sort="Popov, Vsevolod" uniqKey="Popov V" first="Vsevolod" last="Popov">Vsevolod Popov</name>
<name sortKey="Rajsbaum, Ricardo" sort="Rajsbaum, Ricardo" uniqKey="Rajsbaum R" first="Ricardo" last="Rajsbaum">Ricardo Rajsbaum</name>
<name sortKey="Rodriguez, Sergio E" sort="Rodriguez, Sergio E" uniqKey="Rodriguez S" first="Sergio E" last="Rodriguez">Sergio E. Rodriguez</name>
<name sortKey="Rossi, Shannan" sort="Rossi, Shannan" uniqKey="Rossi S" first="Shannan" last="Rossi">Shannan Rossi</name>
<name sortKey="Russell, William K" sort="Russell, William K" uniqKey="Russell W" first="William K" last="Russell">William K. Russell</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002320 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 002320 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:31462558 |texte= Peptidoglycan-Associated Cyclic Lipopeptide Disrupts Viral Infectivity. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:31462558" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |