A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests
Identifieur interne : 000938 ( Ncbi/Checkpoint ); précédent : 000937; suivant : 000939A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests
Auteurs : Zehra Banu Bah I ; Aligül Büyükaksoy ; Sinan Mert Ölmezcan ; Fethi Im Ek ; Muhammed Hasan Aslan ; Ahmet Yavuz OralSource :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2009.
Abstract
SiO2-TiO2 thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the
Url:
DOI: 10.3390/s90604890
PubMed: 22408559
PubMed Central: 3291944
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from <italic>E. coli</italic>
O157:H7: Elementary Sensitivity Tests</title>
<author><name sortKey="Bah I, Zehra Banu" sort="Bah I, Zehra Banu" uniqKey="Bah I Z" first="Zehra Banu" last="Bah I">Zehra Banu Bah I</name>
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<author><name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name>
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<author><name sortKey="Olmezcan, Sinan Mert" sort="Olmezcan, Sinan Mert" uniqKey="Olmezcan S" first="Sinan Mert" last="Ölmezcan">Sinan Mert Ölmezcan</name>
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<author><name sortKey=" Im Ek, Fethi" sort=" Im Ek, Fethi" uniqKey=" Im Ek F" first="Fethi" last=" Im Ek">Fethi Im Ek</name>
<affiliation><nlm:aff id="af3-sensors-09-04890">Nanotechnology Laboratory, Doga Nanobiotech, Istanbul, Turkey; E-Mail:<email>fsimsek@dogananobiotech.net</email>
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<author><name sortKey="Aslan, Muhammed Hasan" sort="Aslan, Muhammed Hasan" uniqKey="Aslan M" first="Muhammed Hasan" last="Aslan">Muhammed Hasan Aslan</name>
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<author><name sortKey="Oral, Ahmet Yavuz" sort="Oral, Ahmet Yavuz" uniqKey="Oral A" first="Ahmet Yavuz" last="Oral">Ahmet Yavuz Oral</name>
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(A.B.);<email>mertolmezcan@gmail.com</email>
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<front><div type="abstract" xml:lang="en"><p>SiO<sub>2</sub>
-TiO<sub>2</sub>
thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the <italic>E. coli</italic>
O157:H7 EDL933 species using strands of 20-mers (5′-TAATATCGGTTGCGGAGGTG -3′) sequence.</p>
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<name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name>
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