A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests.
Identifieur interne : 002042 ( PubMed/Corpus ); précédent : 002041; suivant : 002043A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests.
Auteurs : Zehra Banu Bah I ; Aligül Büyükaksoy ; Sinan Mert Olmezcan ; Fethi Sim Ek ; Muhammed Hasan Aslan ; Ahmet Yavuz OralSource :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2009.
Abstract
SiO(2)-TiO(2) thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the E. coli O157:H7 EDL933 species using strands of 20-mers (5'-TAATATCGGTTGCGGAGGTG -3') sequence.
DOI: 10.3390/s90604890
PubMed: 22408559
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The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the E. coli O157:H7 EDL933 species using strands of 20-mers (5'-TAATATCGGTTGCGGAGGTG -3') sequence.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">22408559</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1424-8220</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>6</Issue><PubDate><Year>2009</Year></PubDate></JournalIssue><Title>Sensors (Basel, Switzerland)</Title><ISOAbbreviation>Sensors (Basel)</ISOAbbreviation></Journal><ArticleTitle>A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests.</ArticleTitle><Pagination><MedlinePgn>4890-900</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3390/s90604890</ELocationID><Abstract><AbstractText>SiO(2)-TiO(2) thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the E. coli O157:H7 EDL933 species using strands of 20-mers (5'-TAATATCGGTTGCGGAGGTG -3') sequence.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bahşi</LastName><ForeName>Zehra Banu</ForeName><Initials>ZB</Initials><AffiliationInfo><Affiliation>Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail: bbahsi@gyte.edu.tr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Büyükaksoy</LastName><ForeName>Aligül</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Olmezcan</LastName><ForeName>Sinan Mert</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Simşek</LastName><ForeName>Fethi</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Aslan</LastName><ForeName>Muhammed Hasan</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Oral</LastName><ForeName>Ahmet Yavuz</ForeName><Initials>AY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>06</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Sensors (Basel)</MedlineTA><NlmUniqueID>101204366</NlmUniqueID><ISSNLinking>1424-8220</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">optical DNA biosensor</Keyword><Keyword MajorTopicYN="Y">prism coupler</Keyword><Keyword MajorTopicYN="Y">sol-gel</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>04</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>06</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>3</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22408559</ArticleId><ArticleId IdType="doi">10.3390/s90604890</ArticleId><ArticleId IdType="pii">s90604890</ArticleId><ArticleId IdType="pmc">PMC3291944</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biosens Bioelectron. 2008 May 15;23(10):1572-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18308536</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosens Bioelectron. 2005 Dec 15;21(6):881-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16257656</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosens Bioelectron. 2008 Dec 1;24(4):787-92</Citation><ArticleIdList><ArticleId IdType="pubmed">18692388</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chim Acta. 2008 Jul 14;620(1-2):8-26</Citation><ArticleIdList><ArticleId IdType="pubmed">18558119</ArticleId></ArticleIdList></Reference><Reference><Citation>J Fluoresc. 2005 Mar;15(2):161-70</Citation><ArticleIdList><ArticleId IdType="pubmed">15883771</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Opt. 1973 Dec 1;12(12):2901-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20125894</ArticleId></ArticleIdList></Reference><Reference><Citation>Talanta. 2005 Jan 30;65(2):291-7</Citation><ArticleIdList><ArticleId IdType="pubmed">18969798</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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