A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests
Identifieur interne : 000938 ( Ncbi/Merge ); précédent : 000937; suivant : 000939A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests
Auteurs : Zehra Banu Bah I ; Aligül Büyükaksoy ; Sinan Mert Ölmezcan ; Fethi Im Ek ; Muhammed Hasan Aslan ; Ahmet Yavuz OralSource :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2009.
Abstract
SiO2-TiO2 thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the
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DOI: 10.3390/s90604890
PubMed: 22408559
PubMed Central: 3291944
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E-Mail:<email>bbahsi@gyte.edu.tr</email></nlm:aff></affiliation></author><author><name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author><author><name sortKey="Olmezcan, Sinan Mert" sort="Olmezcan, Sinan Mert" uniqKey="Olmezcan S" first="Sinan Mert" last="Ölmezcan">Sinan Mert Ölmezcan</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author><author><name sortKey=" Im Ek, Fethi" sort=" Im Ek, Fethi" uniqKey=" Im Ek F" first="Fethi" last=" Im Ek">Fethi Im Ek</name><affiliation><nlm:aff id="af3-sensors-09-04890">Nanotechnology Laboratory, Doga Nanobiotech, Istanbul, Turkey; E-Mail:<email>fsimsek@dogananobiotech.net</email></nlm:aff></affiliation></author><author><name sortKey="Aslan, Muhammed Hasan" sort="Aslan, Muhammed Hasan" uniqKey="Aslan M" first="Muhammed Hasan" last="Aslan">Muhammed Hasan Aslan</name><affiliation><nlm:aff id="af4-sensors-09-04890">Dept of Physics, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail:<email>maslan@gyte.edu.tr</email></nlm:aff></affiliation></author><author><name sortKey="Oral, Ahmet Yavuz" sort="Oral, Ahmet Yavuz" uniqKey="Oral A" first="Ahmet Yavuz" last="Oral">Ahmet Yavuz Oral</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author></analytic><series><title level="j">Sensors (Basel, Switzerland)</title><idno type="eISSN">1424-8220</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>SiO<sub>2</sub>-TiO<sub>2</sub> thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the <italic>E. coli</italic> O157:H7 EDL933 species using strands of 20-mers (5′-TAATATCGGTTGCGGAGGTG -3′) sequence.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lawrence, C R" uniqKey="Lawrence C">C.R. Lawrence</name></author><author><name sortKey="Geddes, N J" uniqKey="Geddes N">N.J. Geddes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez Mozaz, S" uniqKey="Rodriguez Mozaz S">S. Rodriguez-Mozaz</name></author><author><name sortKey="De Alda, M J L" uniqKey="De Alda M">M.J.L. de Alda</name></author><author><name sortKey="Marco, M P" uniqKey="Marco M">M.P. Marco</name></author><author><name sortKey="Barcel, D" uniqKey="Barcel D">D. 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Weiss</name></author></analytic></biblStruct></listBibl></div1></back></TEI><double pmid="22408559"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from <italic>E. coli</italic> O157:H7: Elementary Sensitivity Tests</title><author><name sortKey="Bah I, Zehra Banu" sort="Bah I, Zehra Banu" uniqKey="Bah I Z" first="Zehra Banu" last="Bah I">Zehra Banu Bah I</name><affiliation><nlm:aff id="af1-sensors-09-04890">Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail:<email>bbahsi@gyte.edu.tr</email></nlm:aff></affiliation></author><author><name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author><author><name sortKey="Olmezcan, Sinan Mert" sort="Olmezcan, Sinan Mert" uniqKey="Olmezcan S" first="Sinan Mert" last="Ölmezcan">Sinan Mert Ölmezcan</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author><author><name sortKey=" Im Ek, Fethi" sort=" Im Ek, Fethi" uniqKey=" Im Ek F" first="Fethi" last=" Im Ek">Fethi Im Ek</name><affiliation><nlm:aff id="af3-sensors-09-04890">Nanotechnology Laboratory, Doga Nanobiotech, Istanbul, Turkey; E-Mail:<email>fsimsek@dogananobiotech.net</email></nlm:aff></affiliation></author><author><name sortKey="Aslan, Muhammed Hasan" sort="Aslan, Muhammed Hasan" uniqKey="Aslan M" first="Muhammed Hasan" last="Aslan">Muhammed Hasan Aslan</name><affiliation><nlm:aff id="af4-sensors-09-04890">Dept of Physics, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail:<email>maslan@gyte.edu.tr</email></nlm:aff></affiliation></author><author><name sortKey="Oral, Ahmet Yavuz" sort="Oral, Ahmet Yavuz" uniqKey="Oral A" first="Ahmet Yavuz" last="Oral">Ahmet Yavuz Oral</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22408559</idno><idno type="pmc">3291944</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291944</idno><idno type="RBID">PMC:3291944</idno><idno type="doi">10.3390/s90604890</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">001146</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001146</idno><idno type="wicri:Area/Pmc/Curation">001146</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001146</idno><idno type="wicri:Area/Pmc/Checkpoint">001383</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001383</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from <italic>E. coli</italic> O157:H7: Elementary Sensitivity Tests</title><author><name sortKey="Bah I, Zehra Banu" sort="Bah I, Zehra Banu" uniqKey="Bah I Z" first="Zehra Banu" last="Bah I">Zehra Banu Bah I</name><affiliation><nlm:aff id="af1-sensors-09-04890">Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail:<email>bbahsi@gyte.edu.tr</email></nlm:aff></affiliation></author><author><name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author><author><name sortKey="Olmezcan, Sinan Mert" sort="Olmezcan, Sinan Mert" uniqKey="Olmezcan S" first="Sinan Mert" last="Ölmezcan">Sinan Mert Ölmezcan</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author><author><name sortKey=" Im Ek, Fethi" sort=" Im Ek, Fethi" uniqKey=" Im Ek F" first="Fethi" last=" Im Ek">Fethi Im Ek</name><affiliation><nlm:aff id="af3-sensors-09-04890">Nanotechnology Laboratory, Doga Nanobiotech, Istanbul, Turkey; E-Mail:<email>fsimsek@dogananobiotech.net</email></nlm:aff></affiliation></author><author><name sortKey="Aslan, Muhammed Hasan" sort="Aslan, Muhammed Hasan" uniqKey="Aslan M" first="Muhammed Hasan" last="Aslan">Muhammed Hasan Aslan</name><affiliation><nlm:aff id="af4-sensors-09-04890">Dept of Physics, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail:<email>maslan@gyte.edu.tr</email></nlm:aff></affiliation></author><author><name sortKey="Oral, Ahmet Yavuz" sort="Oral, Ahmet Yavuz" uniqKey="Oral A" first="Ahmet Yavuz" last="Oral">Ahmet Yavuz Oral</name><affiliation><nlm:aff id="af2-sensors-09-04890">Dept of Materials Science and Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mails:<email>aligul@gyte.edu.tr</email> (A.B.);<email>mertolmezcan@gmail.com</email> (S.M.O.)</nlm:aff></affiliation></author></analytic><series><title level="j">Sensors (Basel, Switzerland)</title><idno type="eISSN">1424-8220</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>SiO<sub>2</sub>-TiO<sub>2</sub> thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the <italic>E. coli</italic> O157:H7 EDL933 species using strands of 20-mers (5′-TAATATCGGTTGCGGAGGTG -3′) sequence.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lawrence, C R" uniqKey="Lawrence C">C.R. Lawrence</name></author><author><name sortKey="Geddes, N J" uniqKey="Geddes N">N.J. Geddes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez Mozaz, S" uniqKey="Rodriguez Mozaz S">S. Rodriguez-Mozaz</name></author><author><name sortKey="De Alda, M J L" uniqKey="De Alda M">M.J.L. de Alda</name></author><author><name sortKey="Marco, M P" uniqKey="Marco M">M.P. Marco</name></author><author><name sortKey="Barcel, D" uniqKey="Barcel D">D. 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Xu</name></author><author><name sortKey="Yuan, D R" uniqKey="Yuan D">D.R. Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Festag, G" uniqKey="Festag G">G. Festag</name></author><author><name sortKey="Steinbruck, A" uniqKey="Steinbruck A">A. Steinbrück</name></author><author><name sortKey="Wolff, A" uniqKey="Wolff A">A. Wolff</name></author><author><name sortKey="Csaki, A" uniqKey="Csaki A">A. Csaki</name></author><author><name sortKey="Moller, R" uniqKey="Moller R">R. Möller</name></author><author><name sortKey="Fritzsche, W" uniqKey="Fritzsche W">W. Fritzsche</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rong, G" uniqKey="Rong G">G. Rong</name></author><author><name sortKey="Saarinen, J J" uniqKey="Saarinen J">J.J. Saarinen</name></author><author><name sortKey="Sipe, J E" uniqKey="Sipe J">J.E. Sipe</name></author><author><name sortKey="Weiss, S M" uniqKey="Weiss S">S.M. Weiss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rong, G" uniqKey="Rong G">G. Rong</name></author><author><name sortKey="Najmaie, A" uniqKey="Najmaie A">A. Najmaie</name></author><author><name sortKey="Sipe, J E" uniqKey="Sipe J">J.E. Sipe</name></author><author><name sortKey="Weiss, S M" uniqKey="Weiss S">S.M. Weiss</name></author></analytic></biblStruct></listBibl></div1></back></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests.</title><author><name sortKey="Bah I, Zehra Banu" sort="Bah I, Zehra Banu" uniqKey="Bah I Z" first="Zehra Banu" last="Bah I">Zehra Banu Bah I</name><affiliation wicri:level="1"><nlm:affiliation>Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail: bbahsi@gyte.edu.tr.</nlm:affiliation><country wicri:rule="url">Turquie</country><wicri:regionArea>Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli</wicri:regionArea><wicri:noRegion>Kocaeli</wicri:noRegion></affiliation></author><author><name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name></author><author><name sortKey="Olmezcan, Sinan Mert" sort="Olmezcan, Sinan Mert" uniqKey="Olmezcan S" first="Sinan Mert" last="Olmezcan">Sinan Mert Olmezcan</name></author><author><name sortKey="Sim Ek, Fethi" sort="Sim Ek, Fethi" uniqKey="Sim Ek F" first="Fethi" last="Sim Ek">Fethi Sim Ek</name></author><author><name sortKey="Aslan, Muhammed Hasan" sort="Aslan, Muhammed Hasan" uniqKey="Aslan M" first="Muhammed Hasan" last="Aslan">Muhammed Hasan Aslan</name></author><author><name sortKey="Oral, Ahmet Yavuz" sort="Oral, Ahmet Yavuz" uniqKey="Oral A" first="Ahmet Yavuz" last="Oral">Ahmet Yavuz Oral</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:22408559</idno><idno type="pmid">22408559</idno><idno type="doi">10.3390/s90604890</idno><idno type="wicri:Area/PubMed/Corpus">002042</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002042</idno><idno type="wicri:Area/PubMed/Curation">002042</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002042</idno><idno type="wicri:Area/PubMed/Checkpoint">001F37</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F37</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Novel Label-Free Optical Biosensor Using Synthetic Oligonucleotides from E. coli O157:H7: Elementary Sensitivity Tests.</title><author><name sortKey="Bah I, Zehra Banu" sort="Bah I, Zehra Banu" uniqKey="Bah I Z" first="Zehra Banu" last="Bah I">Zehra Banu Bah I</name><affiliation wicri:level="1"><nlm:affiliation>Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli, Turkey; E-Mail: bbahsi@gyte.edu.tr.</nlm:affiliation><country wicri:rule="url">Turquie</country><wicri:regionArea>Dept of Environmental Engineering, Gebze Institute of Technology, Kocaeli</wicri:regionArea><wicri:noRegion>Kocaeli</wicri:noRegion></affiliation></author><author><name sortKey="Buyukaksoy, Aligul" sort="Buyukaksoy, Aligul" uniqKey="Buyukaksoy A" first="Aligül" last="Büyükaksoy">Aligül Büyükaksoy</name></author><author><name sortKey="Olmezcan, Sinan Mert" sort="Olmezcan, Sinan Mert" uniqKey="Olmezcan S" first="Sinan Mert" last="Olmezcan">Sinan Mert Olmezcan</name></author><author><name sortKey="Sim Ek, Fethi" sort="Sim Ek, Fethi" uniqKey="Sim Ek F" first="Fethi" last="Sim Ek">Fethi Sim Ek</name></author><author><name sortKey="Aslan, Muhammed Hasan" sort="Aslan, Muhammed Hasan" uniqKey="Aslan M" first="Muhammed Hasan" last="Aslan">Muhammed Hasan Aslan</name></author><author><name sortKey="Oral, Ahmet Yavuz" sort="Oral, Ahmet Yavuz" uniqKey="Oral A" first="Ahmet Yavuz" last="Oral">Ahmet Yavuz Oral</name></author></analytic><series><title level="j">Sensors (Basel, Switzerland)</title><idno type="ISSN">1424-8220</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SiO(2)-TiO(2) thin films for use as fiber optic guiding layers of optical DNA biosensors were fabricated by the sol-gel dip coating technique. The chemical structure and the surface morphology of the films were characterized before immobilization. Single probe DNA strands were immobilized on the surface and the porosity of the films before the hybridization process was measured. Refractive index values of the films were measured using a Metricon 2010 prism coupler. On the surface of each film, 12 different spots were taken for measurement and calculation of the mean refractive index values with their standard deviations. The increased refractive index values after the immobilization of single DNA strands indicated that immobilization was successfully achieved. A further refractive index increase after the hybridization with target single DNA strands showed the possibility of detection of the E. coli O157:H7 EDL933 species using strands of 20-mers (5'-TAATATCGGTTGCGGAGGTG -3') sequence.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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