Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
Identifieur interne : 004A79 ( Main/Merge ); précédent : 004A78; suivant : 004A80Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
Auteurs : Y. Kajimura [États-Unis] ; J. Krull ; S. Miyakoshi ; K. Itakura ; H. ToyodaSource :
- Genetic analysis, techniques and applications [ 1050-3862 ] ; 1990.
Descripteurs français
- KwdFr :
- ADN (analyse), Allèles, Antigènes HLA-DQ (génétique), Antigènes HLA-DR (génétique), Données de séquences moléculaires, Humains, Hybridation d'acides nucléiques, Lignée cellulaire, Similitude de séquences d'acides nucléiques, Sondes oligonucléotidiques, Séquence d'acides aminés, Séquence nucléotidique, Température.
- MESH :
- analyse : ADN.
- génétique : Antigènes HLA-DQ, Antigènes HLA-DR.
- Allèles, Données de séquences moléculaires, Humains, Hybridation d'acides nucléiques, Lignée cellulaire, Similitude de séquences d'acides nucléiques, Sondes oligonucléotidiques, Séquence d'acides aminés, Séquence nucléotidique, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA.
- chemical , genetics : HLA-DQ Antigens, HLA-DR Antigens.
- Alleles, Amino Acid Sequence, Base Sequence, Cell Line, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Oligonucleotide Probes, Sequence Homology, Nucleic Acid, Temperature.
Abstract
Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.
DOI: 10.1016/0735-0651(90)90031-a
PubMed: 2206600
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002A25
- to stream PubMed, to step Curation: 002A25
- to stream PubMed, to step Checkpoint: 002870
- to stream Ncbi, to step Merge: 000897
- to stream Ncbi, to step Curation: 000897
- to stream Ncbi, to step Checkpoint: 000897
Links to Exploration step
pubmed:2206600Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.</title><author><name sortKey="Kajimura, Y" sort="Kajimura, Y" uniqKey="Kajimura Y" first="Y" last="Kajimura">Y. Kajimura</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, CA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte</wicri:cityArea></affiliation></author><author><name sortKey="Krull, J" sort="Krull, J" uniqKey="Krull J" first="J" last="Krull">J. Krull</name></author><author><name sortKey="Miyakoshi, S" sort="Miyakoshi, S" uniqKey="Miyakoshi S" first="S" last="Miyakoshi">S. Miyakoshi</name></author><author><name sortKey="Itakura, K" sort="Itakura, K" uniqKey="Itakura K" first="K" last="Itakura">K. Itakura</name></author><author><name sortKey="Toyoda, H" sort="Toyoda, H" uniqKey="Toyoda H" first="H" last="Toyoda">H. Toyoda</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2206600</idno><idno type="pmid">2206600</idno><idno type="doi">10.1016/0735-0651(90)90031-a</idno><idno type="wicri:Area/PubMed/Corpus">002A25</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A25</idno><idno type="wicri:Area/PubMed/Curation">002A25</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A25</idno><idno type="wicri:Area/PubMed/Checkpoint">002870</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002870</idno><idno type="wicri:Area/Ncbi/Merge">000897</idno><idno type="wicri:Area/Ncbi/Curation">000897</idno><idno type="wicri:Area/Ncbi/Checkpoint">000897</idno><idno type="wicri:doubleKey">1050-3862:1990:Kajimura Y:application:of:long</idno><idno type="wicri:Area/Main/Merge">004A79</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.</title><author><name sortKey="Kajimura, Y" sort="Kajimura, Y" uniqKey="Kajimura Y" first="Y" last="Kajimura">Y. Kajimura</name><affiliation wicri:level="2"><nlm:affiliation>Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, CA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte</wicri:cityArea></affiliation></author><author><name sortKey="Krull, J" sort="Krull, J" uniqKey="Krull J" first="J" last="Krull">J. Krull</name></author><author><name sortKey="Miyakoshi, S" sort="Miyakoshi, S" uniqKey="Miyakoshi S" first="S" last="Miyakoshi">S. Miyakoshi</name></author><author><name sortKey="Itakura, K" sort="Itakura, K" uniqKey="Itakura K" first="K" last="Itakura">K. Itakura</name></author><author><name sortKey="Toyoda, H" sort="Toyoda, H" uniqKey="Toyoda H" first="H" last="Toyoda">H. Toyoda</name></author></analytic><series><title level="j">Genetic analysis, techniques and applications</title><idno type="ISSN">1050-3862</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cell Line</term><term>DNA (analysis)</term><term>HLA-DQ Antigens (genetics)</term><term>HLA-DR Antigens (genetics)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotide Probes</term><term>Sequence Homology, Nucleic Acid</term><term>Temperature</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>Allèles</term><term>Antigènes HLA-DQ (génétique)</term><term>Antigènes HLA-DR (génétique)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Hybridation d'acides nucléiques</term><term>Lignée cellulaire</term><term>Similitude de séquences d'acides nucléiques</term><term>Sondes oligonucléotidiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Température</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>HLA-DQ Antigens</term><term>HLA-DR Antigens</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes HLA-DQ</term><term>Antigènes HLA-DR</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cell Line</term><term>Humans</term><term>Molecular Sequence Data</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotide Probes</term><term>Sequence Homology, Nucleic Acid</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allèles</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Hybridation d'acides nucléiques</term><term>Lignée cellulaire</term><term>Similitude de séquences d'acides nucléiques</term><term>Sondes oligonucléotidiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004A79 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004A79 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:2206600
|texte= Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:2206600" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |