Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
Identifieur interne : 002870 ( PubMed/Checkpoint ); précédent : 002869; suivant : 002871Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
Auteurs : Y. Kajimura [États-Unis] ; J. Krull ; S. Miyakoshi ; K. Itakura ; H. ToyodaSource :
- Genetic analysis, techniques and applications [ 1050-3862 ] ; 1990.
Descripteurs français
- KwdFr :
- ADN (analyse), Allèles, Antigènes HLA-DQ (génétique), Antigènes HLA-DR (génétique), Données de séquences moléculaires, Humains, Hybridation d'acides nucléiques, Lignée cellulaire, Similitude de séquences d'acides nucléiques, Sondes oligonucléotidiques, Séquence d'acides aminés, Séquence nucléotidique, Température.
- MESH :
- analyse : ADN.
- génétique : Antigènes HLA-DQ, Antigènes HLA-DR.
- Allèles, Données de séquences moléculaires, Humains, Hybridation d'acides nucléiques, Lignée cellulaire, Similitude de séquences d'acides nucléiques, Sondes oligonucléotidiques, Séquence d'acides aminés, Séquence nucléotidique, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA.
- chemical , genetics : HLA-DQ Antigens, HLA-DR Antigens.
- Alleles, Amino Acid Sequence, Base Sequence, Cell Line, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Oligonucleotide Probes, Sequence Homology, Nucleic Acid, Temperature.
Abstract
Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.
DOI: 10.1016/0735-0651(90)90031-a
PubMed: 2206600
Affiliations:
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Miyakoshi</name></author><author><name sortKey="Itakura, K" sort="Itakura, K" uniqKey="Itakura K" first="K" last="Itakura">K. Itakura</name></author><author><name sortKey="Toyoda, H" sort="Toyoda, H" uniqKey="Toyoda H" first="H" last="Toyoda">H. 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These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2206600</PMID><DateCompleted><Year>1990</Year><Month>11</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>29</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1050-3862</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>4</Issue><PubDate><Year>1990</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Genetic analysis, techniques and applications</Title><ISOAbbreviation>Genet. Anal. Tech. Appl.</ISOAbbreviation></Journal><ArticleTitle>Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.</ArticleTitle><Pagination><MedlinePgn>71-9</MedlinePgn></Pagination><Abstract><AbstractText>Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. 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Itakura</name><name sortKey="Krull, J" sort="Krull, J" uniqKey="Krull J" first="J" last="Krull">J. Krull</name><name sortKey="Miyakoshi, S" sort="Miyakoshi, S" uniqKey="Miyakoshi S" first="S" last="Miyakoshi">S. Miyakoshi</name><name sortKey="Toyoda, H" sort="Toyoda, H" uniqKey="Toyoda H" first="H" last="Toyoda">H. Toyoda</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Kajimura, Y" sort="Kajimura, Y" uniqKey="Kajimura Y" first="Y" last="Kajimura">Y. Kajimura</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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