MersV1, Main, Merge, bibRecord, 004919

Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase

Identifieur interne : 004919 ( Main/Merge ); précédent : 004918; suivant : 004920

Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase

Auteurs : G. Maury [France] ; A. El Alaoui ; F. Morvan ; B. Müller ; J.-L. Imbach ; R. S. Goody

Source :

Biochemical and biophysical research communications [ 0006-291X ] ; 1992.

RBID : Pascal:92-0557956

Descripteurs français

Pascal (Inist)
- Virus HIV1, DNA polymerase RNA-dependent, Oligonucléotide, Interaction moléculaire, Activité matricielle, Cinétique, Activité enzymatique.

English descriptors

KwdEn :
- DNA polymerase RNA-dependent, Enzymatic activity, HIV-1 virus, Kinetics, Molecular interaction, Oligonucleotide, Template activity.

Abstract

We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)₁₄ as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000113
to stream PascalFrancis, to step Curation: 000101
to stream PascalFrancis, to step Checkpoint: 000108

Links to Exploration step

Pascal:92-0557956

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</title>
<author><name sortKey="Maury, G" sort="Maury, G" uniqKey="Maury G" first="G." last="Maury">G. Maury</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>CNRS Univ. Montpellier II, dép. chimie organique fine</s1>
<s2>34095 Montpellier</s2>
<s3>FRA</s3>
<sZ>1 aut., 2 aut., 3 aut., 5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="El Alaoui, A" sort="El Alaoui, A" uniqKey="El Alaoui A" first="A." last="El Alaoui">A. El Alaoui</name>
</author>
<author><name sortKey="Morvan, F" sort="Morvan, F" uniqKey="Morvan F" first="F." last="Morvan">F. Morvan</name>
</author>
<author><name sortKey="Muller, B" sort="Muller, B" uniqKey="Muller B" first="B." last="Müller">B. Müller</name>
</author>
<author><name sortKey="Imbach, J L" sort="Imbach, J L" uniqKey="Imbach J" first="J.-L." last="Imbach">J.-L. Imbach</name>
</author>
<author><name sortKey="Goody, R S" sort="Goody, R S" uniqKey="Goody R" first="R. S." last="Goody">R. S. Goody</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">92-0557956</idno>
<date when="1992">1992</date>
<idno type="stanalyst">PASCAL 92-0557956 INIST</idno>
<idno type="RBID">Pascal:92-0557956</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000113</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000101</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000108</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000108</idno>
<idno type="wicri:doubleKey">0006-291X:1992:Maury G:template:phosphorothioate:oligonucleotides</idno>
<idno type="wicri:Area/Main/Merge">004919</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</title>
<author><name sortKey="Maury, G" sort="Maury, G" uniqKey="Maury G" first="G." last="Maury">G. Maury</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>CNRS Univ. Montpellier II, dép. chimie organique fine</s1>
<s2>34095 Montpellier</s2>
<s3>FRA</s3>
<sZ>1 aut., 2 aut., 3 aut., 5 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="El Alaoui, A" sort="El Alaoui, A" uniqKey="El Alaoui A" first="A." last="El Alaoui">A. El Alaoui</name>
</author>
<author><name sortKey="Morvan, F" sort="Morvan, F" uniqKey="Morvan F" first="F." last="Morvan">F. Morvan</name>
</author>
<author><name sortKey="Muller, B" sort="Muller, B" uniqKey="Muller B" first="B." last="Müller">B. Müller</name>
</author>
<author><name sortKey="Imbach, J L" sort="Imbach, J L" uniqKey="Imbach J" first="J.-L." last="Imbach">J.-L. Imbach</name>
</author>
<author><name sortKey="Goody, R S" sort="Goody, R S" uniqKey="Goody R" first="R. S." last="Goody">R. S. Goody</name>
</author>
</analytic>
<series><title level="j" type="main">Biochemical and biophysical research communications</title>
<title level="j" type="abbreviated">Biochem. biophys. res. commun.</title>
<idno type="ISSN">0006-291X</idno>
<imprint><date when="1992">1992</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Biochemical and biophysical research communications</title>
<title level="j" type="abbreviated">Biochem. biophys. res. commun.</title>
<idno type="ISSN">0006-291X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA polymerase RNA-dependent</term>
<term>Enzymatic activity</term>
<term>HIV-1 virus</term>
<term>Kinetics</term>
<term>Molecular interaction</term>
<term>Oligonucleotide</term>
<term>Template activity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term>
<term>DNA polymerase RNA-dependent</term>
<term>Oligonucléotide</term>
<term>Interaction moléculaire</term>
<term>Activité matricielle</term>
<term>Cinétique</term>
<term>Activité enzymatique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)<sub>14</sub>
 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Languedoc-Roussillon</li>
<li>Occitanie (région administrative)</li>
</region>
<settlement><li>Montpellier</li>
</settlement>
</list>
<tree><noCountry><name sortKey="El Alaoui, A" sort="El Alaoui, A" uniqKey="El Alaoui A" first="A." last="El Alaoui">A. El Alaoui</name>
<name sortKey="Goody, R S" sort="Goody, R S" uniqKey="Goody R" first="R. S." last="Goody">R. S. Goody</name>
<name sortKey="Imbach, J L" sort="Imbach, J L" uniqKey="Imbach J" first="J.-L." last="Imbach">J.-L. Imbach</name>
<name sortKey="Morvan, F" sort="Morvan, F" uniqKey="Morvan F" first="F." last="Morvan">F. Morvan</name>
<name sortKey="Muller, B" sort="Muller, B" uniqKey="Muller B" first="B." last="Müller">B. Müller</name>
</noCountry>
<country name="France"><region name="Occitanie (région administrative)"><name sortKey="Maury, G" sort="Maury, G" uniqKey="Maury G" first="G." last="Maury">G. Maury</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004919 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004919 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     Pascal:92-0557956
   |texte=   Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase

Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri