Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
Identifieur interne : 000108 ( PascalFrancis/Checkpoint ); précédent : 000107; suivant : 000109Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
Auteurs : G. Maury [France] ; A. El Alaoui ; F. Morvan ; B. Müller ; J.-L. Imbach ; R. S. GoodySource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1992.
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- Pascal (Inist)
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Abstract
We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase
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Morvan</name></author><author><name sortKey="Muller, B" sort="Muller, B" uniqKey="Muller B" first="B." last="Müller">B. Müller</name></author><author><name sortKey="Imbach, J L" sort="Imbach, J L" uniqKey="Imbach J" first="J.-L." last="Imbach">J.-L. Imbach</name></author><author><name sortKey="Goody, R S" sort="Goody, R S" uniqKey="Goody R" first="R. S." last="Goody">R. S. Goody</name></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">92-0557956</idno><date when="1992">1992</date><idno type="stanalyst">PASCAL 92-0557956 INIST</idno><idno type="RBID">Pascal:92-0557956</idno><idno type="wicri:Area/PascalFrancis/Corpus">000113</idno><idno type="wicri:Area/PascalFrancis/Curation">000101</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000108</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000108</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</title><author><name sortKey="Maury, G" sort="Maury, G" uniqKey="Maury G" first="G." last="Maury">G. Maury</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>CNRS Univ. Montpellier II, dép. chimie organique fine</s1><s2>34095 Montpellier</s2><s3>FRA</s3><sZ>1 aut., 2 aut., 3 aut., 5 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="El Alaoui, A" sort="El Alaoui, A" uniqKey="El Alaoui A" first="A." last="El Alaoui">A. El Alaoui</name></author><author><name sortKey="Morvan, F" sort="Morvan, F" uniqKey="Morvan F" first="F." last="Morvan">F. 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Goody</name></author></analytic><series><title level="j" type="main">Biochemical and biophysical research communications</title><title level="j" type="abbreviated">Biochem. biophys. res. commun.</title><idno type="ISSN">0006-291X</idno><imprint><date when="1992">1992</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Biochemical and biophysical research communications</title><title level="j" type="abbreviated">Biochem. biophys. res. commun.</title><idno type="ISSN">0006-291X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA polymerase RNA-dependent</term><term>Enzymatic activity</term><term>HIV-1 virus</term><term>Kinetics</term><term>Molecular interaction</term><term>Oligonucleotide</term><term>Template activity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term><term>DNA polymerase RNA-dependent</term><term>Oligonucléotide</term><term>Interaction moléculaire</term><term>Activité matricielle</term><term>Cinétique</term><term>Activité enzymatique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)<sub>14</sub> as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0006-291X</s0></fA01><fA02 i1="01"><s0>BBRCA9</s0></fA02><fA03 i2="1"><s0>Biochem. biophys. res. commun.</s0></fA03><fA05><s2>186</s2></fA05><fA06><s2>3</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Template. 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El Alaoui</name><name sortKey="Goody, R S" sort="Goody, R S" uniqKey="Goody R" first="R. S." last="Goody">R. S. Goody</name><name sortKey="Imbach, J L" sort="Imbach, J L" uniqKey="Imbach J" first="J.-L." last="Imbach">J.-L. Imbach</name><name sortKey="Morvan, F" sort="Morvan, F" uniqKey="Morvan F" first="F." last="Morvan">F. Morvan</name><name sortKey="Muller, B" sort="Muller, B" uniqKey="Muller B" first="B." last="Müller">B. Müller</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Maury, G" sort="Maury, G" uniqKey="Maury G" first="G." last="Maury">G. Maury</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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