MersV1, Main, Exploration, bibRecordById, pubmed:2908426

CC/GG contacts facilitate the B to A transition of DNA in solution.

Identifieur interne : 004D31 ( Main/Exploration ); précédent : 004D30; suivant : 004D32

CC/GG contacts facilitate the B to A transition of DNA in solution.

Auteurs : L E Minchenkova [Russie] ; A K Schyolkina ; B K Chernov ; V I Ivanov

Source :

Journal of biomolecular structure & dynamics [ 0739-1102 ] ; 1986.

RBID : pubmed:2908426

Descripteurs français

KwdFr :
- ADN, Clostridium perfringens (génétique), Composition en bases nucléiques, Conformation d'acide nucléique, Dénaturation d'acide nucléique, Escherichia coli (génétique), Mycobacterium (génétique), Solutions, Séquence nucléotidique, Température élevée, Thermodynamique.
MESH :
- génétique : Clostridium perfringens, Escherichia coli, Mycobacterium.
- ADN, Composition en bases nucléiques, Conformation d'acide nucléique, Dénaturation d'acide nucléique, Solutions, Séquence nucléotidique, Température élevée, Thermodynamique.

English descriptors

KwdEn :
- Base Composition, Base Sequence, Clostridium perfringens (genetics), DNA, Escherichia coli (genetics), Hot Temperature, Mycobacterium (genetics), Nucleic Acid Conformation, Nucleic Acid Denaturation, Solutions, Thermodynamics.
MESH :
- chemical : DNA, Solutions.
- genetics : Clostridium perfringens, Escherichia coli, Mycobacterium.
- Base Composition, Base Sequence, Hot Temperature, Nucleic Acid Conformation, Nucleic Acid Denaturation, Thermodynamics.

Abstract

Self-complementary decadeoxynucleotides, CCGATATCGG, CCAGATCTGG, CCCTGCAGGG, GGGGGCCCCC, were designed and synthesized to estimate the A-philic free energy of CC/GG contacts. First, regions of temperature-stability of the double-stranded conformation were determined for each 10-mer. Then, circular dichroism spectra were recorded for the B-family forms at different temperatures, counter-ion concentrations and trifluoroethanol contents. A cooperative change typical of the B-A transition is observed in the CD spectra at a trifluoroethanol content specific for each duplex. The positions of half-transition points were functions not only of the nucleotide sequence but of the duplex length as well: the B to A transitions were hindered in these 10-mers in comparison with a lengthy DNA. The B-phility value was estimated to be 3 kcal/mol of 10-mer. The B-A transition point was shown to drop with an increase in the number of CC/GG contacts in a duplex. The designed 10-mers made it possible to estimate quantitatively the A-phility of CC/GG contact as compared with an average DNA: (FA-FB)CC = 0.2 Kcal/mol, (FA-FB)DNA = 0.7 Kcal/mol.

DOI: 10.1080/07391102.1986.10506362
PubMed: 2908426

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Self-complementary decadeoxynucleotides, CCGATATCGG, CCAGATCTGG, CCCTGCAGGG, GGGGGCCCCC, were designed and synthesized to estimate the A-philic free energy of CC/GG contacts. First, regions of temperature-stability of the double-stranded conformation were determined for each 10-mer. Then, circular dichroism spectra were recorded for the B-family forms at different temperatures, counter-ion concentrations and trifluoroethanol contents. A cooperative change typical of the B-A transition is observed in the CD spectra at a trifluoroethanol content specific for each duplex. The positions of half-transition points were functions not only of the nucleotide sequence but of the duplex length as well: the B to A transitions were hindered in these 10-mers in comparison with a lengthy DNA. The B-phility value was estimated to be 3 kcal/mol of 10-mer. The B-A transition point was shown to drop with an increase in the number of CC/GG contacts in a duplex. The designed 10-mers made it possible to estimate quantitatively the A-phility of CC/GG contact as compared with an average DNA: (FA-FB)CC = 0.2 Kcal/mol, (FA-FB)DNA = 0.7 Kcal/mol.</div>
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Serveur d'exploration MERS

CC/GG contacts facilitate the B to A transition of DNA in solution.

CC/GG contacts facilitate the B to A transition of DNA in solution.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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