CC/GG contacts facilitate the B to A transition of DNA in solution.
Identifieur interne : 002A80 ( PubMed/Corpus ); précédent : 002A79; suivant : 002A81CC/GG contacts facilitate the B to A transition of DNA in solution.
Auteurs : L E Minchenkova ; A K Schyolkina ; B K Chernov ; V I IvanovSource :
- Journal of biomolecular structure & dynamics [ 0739-1102 ] ; 1986.
English descriptors
- KwdEn :
- MESH :
- chemical : DNA, Solutions.
- genetics : Clostridium perfringens, Escherichia coli, Mycobacterium.
- Base Composition, Base Sequence, Hot Temperature, Nucleic Acid Conformation, Nucleic Acid Denaturation, Thermodynamics.
Abstract
Self-complementary decadeoxynucleotides, CCGATATCGG, CCAGATCTGG, CCCTGCAGGG, GGGGGCCCCC, were designed and synthesized to estimate the A-philic free energy of CC/GG contacts. First, regions of temperature-stability of the double-stranded conformation were determined for each 10-mer. Then, circular dichroism spectra were recorded for the B-family forms at different temperatures, counter-ion concentrations and trifluoroethanol contents. A cooperative change typical of the B-A transition is observed in the CD spectra at a trifluoroethanol content specific for each duplex. The positions of half-transition points were functions not only of the nucleotide sequence but of the duplex length as well: the B to A transitions were hindered in these 10-mers in comparison with a lengthy DNA. The B-phility value was estimated to be 3 kcal/mol of 10-mer. The B-A transition point was shown to drop with an increase in the number of CC/GG contacts in a duplex. The designed 10-mers made it possible to estimate quantitatively the A-phility of CC/GG contact as compared with an average DNA: (FA-FB)CC = 0.2 Kcal/mol, (FA-FB)DNA = 0.7 Kcal/mol.
DOI: 10.1080/07391102.1986.10506362
PubMed: 2908426
Links to Exploration step
pubmed:2908426Le document en format XML
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<author><name sortKey="Minchenkova, L E" sort="Minchenkova, L E" uniqKey="Minchenkova L" first="L E" last="Minchenkova">L E Minchenkova</name>
<affiliation><nlm:affiliation>Institute of Molecular Biology, USSR Academy of Sciences, Moscow.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Schyolkina, A K" sort="Schyolkina, A K" uniqKey="Schyolkina A" first="A K" last="Schyolkina">A K Schyolkina</name>
</author>
<author><name sortKey="Chernov, B K" sort="Chernov, B K" uniqKey="Chernov B" first="B K" last="Chernov">B K Chernov</name>
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<author><name sortKey="Ivanov, V I" sort="Ivanov, V I" uniqKey="Ivanov V" first="V I" last="Ivanov">V I Ivanov</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">CC/GG contacts facilitate the B to A transition of DNA in solution.</title>
<author><name sortKey="Minchenkova, L E" sort="Minchenkova, L E" uniqKey="Minchenkova L" first="L E" last="Minchenkova">L E Minchenkova</name>
<affiliation><nlm:affiliation>Institute of Molecular Biology, USSR Academy of Sciences, Moscow.</nlm:affiliation>
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<author><name sortKey="Schyolkina, A K" sort="Schyolkina, A K" uniqKey="Schyolkina A" first="A K" last="Schyolkina">A K Schyolkina</name>
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<author><name sortKey="Chernov, B K" sort="Chernov, B K" uniqKey="Chernov B" first="B K" last="Chernov">B K Chernov</name>
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<author><name sortKey="Ivanov, V I" sort="Ivanov, V I" uniqKey="Ivanov V" first="V I" last="Ivanov">V I Ivanov</name>
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<series><title level="j">Journal of biomolecular structure & dynamics</title>
<idno type="ISSN">0739-1102</idno>
<imprint><date when="1986" type="published">1986</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Composition</term>
<term>Base Sequence</term>
<term>Clostridium perfringens (genetics)</term>
<term>DNA</term>
<term>Escherichia coli (genetics)</term>
<term>Hot Temperature</term>
<term>Mycobacterium (genetics)</term>
<term>Nucleic Acid Conformation</term>
<term>Nucleic Acid Denaturation</term>
<term>Solutions</term>
<term>Thermodynamics</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA</term>
<term>Solutions</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Clostridium perfringens</term>
<term>Escherichia coli</term>
<term>Mycobacterium</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Composition</term>
<term>Base Sequence</term>
<term>Hot Temperature</term>
<term>Nucleic Acid Conformation</term>
<term>Nucleic Acid Denaturation</term>
<term>Thermodynamics</term>
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<front><div type="abstract" xml:lang="en">Self-complementary decadeoxynucleotides, CCGATATCGG, CCAGATCTGG, CCCTGCAGGG, GGGGGCCCCC, were designed and synthesized to estimate the A-philic free energy of CC/GG contacts. First, regions of temperature-stability of the double-stranded conformation were determined for each 10-mer. Then, circular dichroism spectra were recorded for the B-family forms at different temperatures, counter-ion concentrations and trifluoroethanol contents. A cooperative change typical of the B-A transition is observed in the CD spectra at a trifluoroethanol content specific for each duplex. The positions of half-transition points were functions not only of the nucleotide sequence but of the duplex length as well: the B to A transitions were hindered in these 10-mers in comparison with a lengthy DNA. The B-phility value was estimated to be 3 kcal/mol of 10-mer. The B-A transition point was shown to drop with an increase in the number of CC/GG contacts in a duplex. The designed 10-mers made it possible to estimate quantitatively the A-phility of CC/GG contact as compared with an average DNA: (FA-FB)CC = 0.2 Kcal/mol, (FA-FB)DNA = 0.7 Kcal/mol.</div>
</front>
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<DateCompleted><Year>1990</Year>
<Month>03</Month>
<Day>27</Day>
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<DateRevised><Year>2008</Year>
<Month>11</Month>
<Day>21</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0739-1102</ISSN>
<JournalIssue CitedMedium="Print"><Volume>4</Volume>
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<PubDate><Year>1986</Year>
<Month>Dec</Month>
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<Title>Journal of biomolecular structure & dynamics</Title>
<ISOAbbreviation>J. Biomol. Struct. Dyn.</ISOAbbreviation>
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<ArticleTitle>CC/GG contacts facilitate the B to A transition of DNA in solution.</ArticleTitle>
<Pagination><MedlinePgn>463-76</MedlinePgn>
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<Abstract><AbstractText>Self-complementary decadeoxynucleotides, CCGATATCGG, CCAGATCTGG, CCCTGCAGGG, GGGGGCCCCC, were designed and synthesized to estimate the A-philic free energy of CC/GG contacts. First, regions of temperature-stability of the double-stranded conformation were determined for each 10-mer. Then, circular dichroism spectra were recorded for the B-family forms at different temperatures, counter-ion concentrations and trifluoroethanol contents. A cooperative change typical of the B-A transition is observed in the CD spectra at a trifluoroethanol content specific for each duplex. The positions of half-transition points were functions not only of the nucleotide sequence but of the duplex length as well: the B to A transitions were hindered in these 10-mers in comparison with a lengthy DNA. The B-phility value was estimated to be 3 kcal/mol of 10-mer. The B-A transition point was shown to drop with an increase in the number of CC/GG contacts in a duplex. The designed 10-mers made it possible to estimate quantitatively the A-phility of CC/GG contact as compared with an average DNA: (FA-FB)CC = 0.2 Kcal/mol, (FA-FB)DNA = 0.7 Kcal/mol.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Minchenkova</LastName>
<ForeName>L E</ForeName>
<Initials>LE</Initials>
<AffiliationInfo><Affiliation>Institute of Molecular Biology, USSR Academy of Sciences, Moscow.</Affiliation>
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<Author ValidYN="Y"><LastName>Schyolkina</LastName>
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<Author ValidYN="Y"><LastName>Chernov</LastName>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D001482" MajorTopicYN="N">Base Composition</DescriptorName>
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<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
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<MeshHeading><DescriptorName UI="D003016" MajorTopicYN="N">Clostridium perfringens</DescriptorName>
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<MeshHeading><DescriptorName UI="D004247" MajorTopicYN="Y">DNA</DescriptorName>
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<MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D009690" MajorTopicYN="Y">Nucleic Acid Conformation</DescriptorName>
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<MeshHeading><DescriptorName UI="D009691" MajorTopicYN="N">Nucleic Acid Denaturation</DescriptorName>
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<MeshHeading><DescriptorName UI="D012996" MajorTopicYN="N">Solutions</DescriptorName>
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<MeshHeading><DescriptorName UI="D013816" MajorTopicYN="N">Thermodynamics</DescriptorName>
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