MersV1, Main, Exploration, bibRecord, 004D30

Curved DNA: design, synthesis, and circularization.

Identifieur interne : 004D30 ( Main/Exploration ); précédent : 004D29; suivant : 004D31

Curved DNA: design, synthesis, and circularization.

Auteurs : L. Ulanovsky ; M. Bodner ; E N Trifonov ; M. Choder

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1986.

RBID : pubmed:3456570

Descripteurs français

KwdFr :
- ADN (synthèse chimique), ADN circulaire (synthèse chimique), Conformation d'acide nucléique, Masse moléculaire, Oligodésoxyribonucléotides (synthèse chimique), Électrophorèse sur gel de polyacrylamide.
MESH :
- synthèse chimique : ADN, ADN circulaire, Oligodésoxyribonucléotides.
- Conformation d'acide nucléique, Masse moléculaire, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- DNA (chemical synthesis), DNA, Circular (chemical synthesis), Electrophoresis, Polyacrylamide Gel, Molecular Weight, Nucleic Acid Conformation, Oligodeoxyribonucleotides (chemical synthesis).
MESH :
- chemical , chemical synthesis : DNA, DNA, Circular, Oligodeoxyribonucleotides.
- Electrophoresis, Polyacrylamide Gel, Molecular Weight, Nucleic Acid Conformation.

Abstract

Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide gel electrophoresis of the ligation products showed two distinct families of spots: (i) noncircular oligo(precursor)s of 21 to 231 bp (1- to 11-mers) and (ii) four circles from 105 to 168 bp (eluted and analyzed by denaturing gel electrophoresis). The noncircular oligomers exhibited anomalously slow migration, as if they were as much as three times longer than they actually are. The amount of circular products peaked sharply at approximately equal to 126 bp, near which size the circles have been estimated to be nonconstrained both torsionally and in terms of bending. The nonconstrained circularization provides a technique for the direct measurement of the inherent curvature of DNA in solution. From the size of the circles, an estimate of 8.7 degrees is obtained for the absolute value of the AA X TT wedge angle (roll and tilt combined).

DOI: 10.1073/pnas.83.4.862
PubMed: 3456570

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Curved DNA: design, synthesis, and circularization.</title>
<author><name sortKey="Ulanovsky, L" sort="Ulanovsky, L" uniqKey="Ulanovsky L" first="L" last="Ulanovsky">L. Ulanovsky</name>
</author>
<author><name sortKey="Bodner, M" sort="Bodner, M" uniqKey="Bodner M" first="M" last="Bodner">M. Bodner</name>
</author>
<author><name sortKey="Trifonov, E N" sort="Trifonov, E N" uniqKey="Trifonov E" first="E N" last="Trifonov">E N Trifonov</name>
</author>
<author><name sortKey="Choder, M" sort="Choder, M" uniqKey="Choder M" first="M" last="Choder">M. Choder</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1986">1986</date>
<idno type="RBID">pubmed:3456570</idno>
<idno type="pmid">3456570</idno>
<idno type="doi">10.1073/pnas.83.4.862</idno>
<idno type="wicri:Area/PubMed/Corpus">002A86</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A86</idno>
<idno type="wicri:Area/PubMed/Curation">002A86</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A86</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002927</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002927</idno>
<idno type="wicri:Area/Ncbi/Merge">002842</idno>
<idno type="wicri:Area/Ncbi/Curation">002842</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002842</idno>
<idno type="wicri:doubleKey">0027-8424:1986:Ulanovsky L:curved:dna:design</idno>
<idno type="wicri:Area/Main/Merge">004E10</idno>
<idno type="wicri:Area/Main/Curation">004D30</idno>
<idno type="wicri:Area/Main/Exploration">004D30</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Curved DNA: design, synthesis, and circularization.</title>
<author><name sortKey="Ulanovsky, L" sort="Ulanovsky, L" uniqKey="Ulanovsky L" first="L" last="Ulanovsky">L. Ulanovsky</name>
</author>
<author><name sortKey="Bodner, M" sort="Bodner, M" uniqKey="Bodner M" first="M" last="Bodner">M. Bodner</name>
</author>
<author><name sortKey="Trifonov, E N" sort="Trifonov, E N" uniqKey="Trifonov E" first="E N" last="Trifonov">E N Trifonov</name>
</author>
<author><name sortKey="Choder, M" sort="Choder, M" uniqKey="Choder M" first="M" last="Choder">M. Choder</name>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<imprint><date when="1986" type="published">1986</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA (chemical synthesis)</term>
<term>DNA, Circular (chemical synthesis)</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Molecular Weight</term>
<term>Nucleic Acid Conformation</term>
<term>Oligodeoxyribonucleotides (chemical synthesis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (synthèse chimique)</term>
<term>ADN circulaire (synthèse chimique)</term>
<term>Conformation d'acide nucléique</term>
<term>Masse moléculaire</term>
<term>Oligodésoxyribonucléotides (synthèse chimique)</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>DNA</term>
<term>DNA, Circular</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>ADN</term>
<term>ADN circulaire</term>
<term>Oligodésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term>
<term>Molecular Weight</term>
<term>Nucleic Acid Conformation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Conformation d'acide nucléique</term>
<term>Masse moléculaire</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide gel electrophoresis of the ligation products showed two distinct families of spots: (i) noncircular oligo(precursor)s of 21 to 231 bp (1- to 11-mers) and (ii) four circles from 105 to 168 bp (eluted and analyzed by denaturing gel electrophoresis). The noncircular oligomers exhibited anomalously slow migration, as if they were as much as three times longer than they actually are. The amount of circular products peaked sharply at approximately equal to 126 bp, near which size the circles have been estimated to be nonconstrained both torsionally and in terms of bending. The nonconstrained circularization provides a technique for the direct measurement of the inherent curvature of DNA in solution. From the size of the circles, an estimate of 8.7 degrees is obtained for the absolute value of the AA X TT wedge angle (roll and tilt combined).</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Bodner, M" sort="Bodner, M" uniqKey="Bodner M" first="M" last="Bodner">M. Bodner</name>
<name sortKey="Choder, M" sort="Choder, M" uniqKey="Choder M" first="M" last="Choder">M. Choder</name>
<name sortKey="Trifonov, E N" sort="Trifonov, E N" uniqKey="Trifonov E" first="E N" last="Trifonov">E N Trifonov</name>
<name sortKey="Ulanovsky, L" sort="Ulanovsky, L" uniqKey="Ulanovsky L" first="L" last="Ulanovsky">L. Ulanovsky</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004D30 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004D30 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:3456570
   |texte=   Curved DNA: design, synthesis, and circularization.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:3456570" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

Serveur d'exploration MERS

Curved DNA: design, synthesis, and circularization.

Curved DNA: design, synthesis, and circularization.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.