Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0
Identifieur interne : 005367 ( Main/Exploration ); précédent : 005366; suivant : 005368Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0
Auteurs : Leonie-Sophie Hecht [Allemagne] ; Angeles Jurado-Jimenez [Allemagne] ; Markus Hess [Allemagne] ; Hussein El Halas [Allemagne] ; Gregor Bochenek [Allemagne] ; Hala Mohammed [Arabie saoudite] ; Fahd Alzahrani [Arabie saoudite] ; Mohammed O. Asiri [Arabie saoudite] ; Rami Hasan [Arabie saoudite] ; Aref Alamri [Arabie saoudite] ; Sultan Alotaibi [Arabie saoudite]Source :
- Future Microbiology [ 1746-0913 ] ; ????.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Arabie saoudite, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Protéines nucléocapside (génétique), Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines nucléocapside.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- Arabie saoudite, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Nucleocapsid Proteins (genetics), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction, Saudi Arabia, Sensitivity and Specificity.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, RNA, Viral.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques.
- virology : Coronavirus Infections.
- Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene.
33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV
18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results.
The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.
Url:
DOI: 10.2217/fmb-2019-0067
PubMed: 31271059
PubMed Central: 7079715
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000692
- to stream Pmc, to step Curation: 000692
- to stream Pmc, to step Checkpoint: 001438
- to stream PubMed, to step Corpus: 000490
- to stream PubMed, to step Curation: 000490
- to stream PubMed, to step Checkpoint: 000340
- to stream Ncbi, to step Merge: 002267
- to stream Ncbi, to step Curation: 002267
- to stream Ncbi, to step Checkpoint: 002267
- to stream Main, to step Merge: 005447
- to stream Main, to step Curation: 005367
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Verification and diagnostic evaluation of the RealStar<sup>®</sup>
Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0</title>
<author><name sortKey="Hecht, Leonie Sophie" sort="Hecht, Leonie Sophie" uniqKey="Hecht L" first="Leonie-Sophie" last="Hecht">Leonie-Sophie Hecht</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jurado Jimenez, Angeles" sort="Jurado Jimenez, Angeles" uniqKey="Jurado Jimenez A" first="Angeles" last="Jurado-Jimenez">Angeles Jurado-Jimenez</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hess, Markus" sort="Hess, Markus" uniqKey="Hess M" first="Markus" last="Hess">Markus Hess</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Halas, Hussein El" sort="Halas, Hussein El" uniqKey="Halas H" first="Hussein El" last="Halas">Hussein El Halas</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bochenek, Gregor" sort="Bochenek, Gregor" uniqKey="Bochenek G" first="Gregor" last="Bochenek">Gregor Bochenek</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mohammed, Hala" sort="Mohammed, Hala" uniqKey="Mohammed H" first="Hala" last="Mohammed">Hala Mohammed</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alzahrani, Fahd" sort="Alzahrani, Fahd" uniqKey="Alzahrani F" first="Fahd" last="Alzahrani">Fahd Alzahrani</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Asiri, Mohammed O" sort="Asiri, Mohammed O" uniqKey="Asiri M" first="Mohammed O" last="Asiri">Mohammed O. Asiri</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hasan, Rami" sort="Hasan, Rami" uniqKey="Hasan R" first="Rami" last="Hasan">Rami Hasan</name>
<affiliation wicri:level="1"><nlm:aff id="AFF3">King Fahad Medical City, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alamri, Aref" sort="Alamri, Aref" uniqKey="Alamri A" first="Aref" last="Alamri">Aref Alamri</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alotaibi, Sultan" sort="Alotaibi, Sultan" uniqKey="Alotaibi S" first="Sultan" last="Alotaibi">Sultan Alotaibi</name>
<affiliation wicri:level="1"><nlm:aff id="AFF3">King Fahad Medical City, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">31271059</idno>
<idno type="pmc">7079715</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7079715</idno>
<idno type="RBID">PMC:7079715</idno>
<idno type="doi">10.2217/fmb-2019-0067</idno>
<date when="????">????</date>
<idno type="wicri:Area/Pmc/Corpus">000692</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000692</idno>
<idno type="wicri:Area/Pmc/Curation">000692</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000692</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001438</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001438</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:31271059</idno>
<idno type="wicri:Area/PubMed/Corpus">000490</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000490</idno>
<idno type="wicri:Area/PubMed/Curation">000490</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000490</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000340</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000340</idno>
<idno type="wicri:Area/Ncbi/Merge">002267</idno>
<idno type="wicri:Area/Ncbi/Curation">002267</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002267</idno>
<idno type="wicri:doubleKey">1746-0913:????:Hecht L:verification:and:diagnostic</idno>
<idno type="wicri:Area/Main/Merge">005447</idno>
<idno type="wicri:Area/Main/Curation">005367</idno>
<idno type="wicri:Area/Main/Exploration">005367</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Verification and diagnostic evaluation of the RealStar<sup>®</sup>
Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0</title>
<author><name sortKey="Hecht, Leonie Sophie" sort="Hecht, Leonie Sophie" uniqKey="Hecht L" first="Leonie-Sophie" last="Hecht">Leonie-Sophie Hecht</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Jurado Jimenez, Angeles" sort="Jurado Jimenez, Angeles" uniqKey="Jurado Jimenez A" first="Angeles" last="Jurado-Jimenez">Angeles Jurado-Jimenez</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hess, Markus" sort="Hess, Markus" uniqKey="Hess M" first="Markus" last="Hess">Markus Hess</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Halas, Hussein El" sort="Halas, Hussein El" uniqKey="Halas H" first="Hussein El" last="Halas">Hussein El Halas</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bochenek, Gregor" sort="Bochenek, Gregor" uniqKey="Bochenek G" first="Gregor" last="Bochenek">Gregor Bochenek</name>
<affiliation wicri:level="3"><nlm:aff id="AFF1">aItona Diagnostics GmbH, Hamburg, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea>
<placeName><settlement type="city">Hambourg</settlement>
<region type="land" nuts="2">Hambourg</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mohammed, Hala" sort="Mohammed, Hala" uniqKey="Mohammed H" first="Hala" last="Mohammed">Hala Mohammed</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alzahrani, Fahd" sort="Alzahrani, Fahd" uniqKey="Alzahrani F" first="Fahd" last="Alzahrani">Fahd Alzahrani</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Asiri, Mohammed O" sort="Asiri, Mohammed O" uniqKey="Asiri M" first="Mohammed O" last="Asiri">Mohammed O. Asiri</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hasan, Rami" sort="Hasan, Rami" uniqKey="Hasan R" first="Rami" last="Hasan">Rami Hasan</name>
<affiliation wicri:level="1"><nlm:aff id="AFF3">King Fahad Medical City, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alamri, Aref" sort="Alamri, Aref" uniqKey="Alamri A" first="Aref" last="Alamri">Aref Alamri</name>
<affiliation wicri:level="1"><nlm:aff id="AFF2">Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alotaibi, Sultan" sort="Alotaibi, Sultan" uniqKey="Alotaibi S" first="Sultan" last="Alotaibi">Sultan Alotaibi</name>
<affiliation wicri:level="1"><nlm:aff id="AFF3">King Fahad Medical City, Riyadh, Saudi Arabia</nlm:aff>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Future Microbiology</title>
<idno type="ISSN">1746-0913</idno>
<idno type="eISSN">1746-0921</idno>
<imprint><date when="????">????</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Humans</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term>
<term>Molecular Diagnostic Techniques (methods)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>RNA, Viral (genetics)</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Saudi Arabia</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>Arabie saoudite</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term>
<term>Humains</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Sensibilité et spécificité</term>
<term>Techniques de diagnostic moléculaire ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>Saudi Arabia</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Arabie saoudite</term>
<term>Humains</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Sensibilité et spécificité</term>
<term>Techniques de diagnostic moléculaire</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Aim:</title>
<p>We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene.</p>
</sec>
<sec><title>Material & methods:</title>
<p>33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV <italic>orf1a</italic>
and <italic>upE</italic>
, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO.</p>
</sec>
<sec><title>Results:</title>
<p>18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results.</p>
</sec>
<sec><title>Conclusion:</title>
<p>The results suggest, that the RealStar<sup>®</sup>
MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ogden, Nh" uniqKey="Ogden N">NH Ogden</name>
</author>
<author><name sortKey="Abdelmalik, P" uniqKey="Abdelmalik P">P Abdelmalik</name>
</author>
<author><name sortKey="Pulliam, Jrc" uniqKey="Pulliam J">JRC Pulliam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM Poutanen</name>
</author>
<author><name sortKey="Low, De" uniqKey="Low D">DE Low</name>
</author>
<author><name sortKey="Bonnie, H" uniqKey="Bonnie H">H Bonnie</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S Van Boheemen</name>
</author>
<author><name sortKey="Graaf, Md" uniqKey="Graaf M">MD Graaf</name>
</author>
<author><name sortKey="Lauber, C" uniqKey="Lauber C">C Lauber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goldstein, Sa" uniqKey="Goldstein S">SA Goldstein</name>
</author>
<author><name sortKey="Weiss, Sr" uniqKey="Weiss S">SR Weiss</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mackay, Im" uniqKey="Mackay I">IM Mackay</name>
</author>
<author><name sortKey="Arden, Ke" uniqKey="Arden K">KE Arden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Ithete, Nl" uniqKey="Ithete N">NL Ithete</name>
</author>
<author><name sortKey="Richards, Lr" uniqKey="Richards L">LR Richards</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A Zumla</name>
</author>
<author><name sortKey="Hui, Ds" uniqKey="Hui D">DS Hui</name>
</author>
<author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Arabi, Ym" uniqKey="Arabi Y">YM Arabi</name>
</author>
<author><name sortKey="Balkhy, Hh" uniqKey="Balkhy H">HH Balkhy</name>
</author>
<author><name sortKey="Hayden, Fg" uniqKey="Hayden F">FG Hayden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thabet, F" uniqKey="Thabet F">F Thabet</name>
</author>
<author><name sortKey="Chehab, M" uniqKey="Chehab M">M Chehab</name>
</author>
<author><name sortKey="Bafaqih, H" uniqKey="Bafaqih H">H Bafaqih</name>
</author>
<author><name sortKey="Al, Mohaimeed S" uniqKey="Al M">Mohaimeed S Al</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perkins, Md" uniqKey="Perkins M">MD Perkins</name>
</author>
<author><name sortKey="Dye, C" uniqKey="Dye C">C Dye</name>
</author>
<author><name sortKey="Balasegaram, M" uniqKey="Balasegaram M">M Balasegaram</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Müller</name>
</author>
<author><name sortKey="Costabel, U" uniqKey="Costabel U">U Costabel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I Eckerle</name>
</author>
<author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T Bleicker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oh, M D" uniqKey="Oh M">M-D Oh</name>
</author>
<author><name sortKey="Park, Wb" uniqKey="Park W">WB Park</name>
</author>
<author><name sortKey="Park, S W" uniqKey="Park S">S-W Park</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Chan, Jfw" uniqKey="Chan J">JFW Chan</name>
</author>
<author><name sortKey="Lau, Skp" uniqKey="Lau S">SKP Lau</name>
</author>
<author><name sortKey="To, Kkw" uniqKey="To K">KKW To</name>
</author>
<author><name sortKey="Cheng, Vcc" uniqKey="Cheng V">VCC Cheng</name>
</author>
<author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name>
</author>
<author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K-Y Yuen</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Huang, L H" uniqKey="Huang L">L-H Huang</name>
</author>
<author><name sortKey="Lin, P H" uniqKey="Lin P">P-H Lin</name>
</author>
<author><name sortKey="Tsai, K W" uniqKey="Tsai K">K-W Tsai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Olschl Ger, S" uniqKey="Olschl Ger S">S Ölschläger</name>
</author>
<author><name sortKey="Wendtner, C M" uniqKey="Wendtner C">C-M Wendtner</name>
</author>
<author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name>
</author>
<author><name sortKey="Hess, M" uniqKey="Hess M">M Hess</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>Arabie saoudite</li>
</country>
<region><li>Hambourg</li>
</region>
<settlement><li>Hambourg</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Hambourg"><name sortKey="Hecht, Leonie Sophie" sort="Hecht, Leonie Sophie" uniqKey="Hecht L" first="Leonie-Sophie" last="Hecht">Leonie-Sophie Hecht</name>
</region>
<name sortKey="Bochenek, Gregor" sort="Bochenek, Gregor" uniqKey="Bochenek G" first="Gregor" last="Bochenek">Gregor Bochenek</name>
<name sortKey="Halas, Hussein El" sort="Halas, Hussein El" uniqKey="Halas H" first="Hussein El" last="Halas">Hussein El Halas</name>
<name sortKey="Hess, Markus" sort="Hess, Markus" uniqKey="Hess M" first="Markus" last="Hess">Markus Hess</name>
<name sortKey="Jurado Jimenez, Angeles" sort="Jurado Jimenez, Angeles" uniqKey="Jurado Jimenez A" first="Angeles" last="Jurado-Jimenez">Angeles Jurado-Jimenez</name>
</country>
<country name="Arabie saoudite"><noRegion><name sortKey="Mohammed, Hala" sort="Mohammed, Hala" uniqKey="Mohammed H" first="Hala" last="Mohammed">Hala Mohammed</name>
</noRegion>
<name sortKey="Alamri, Aref" sort="Alamri, Aref" uniqKey="Alamri A" first="Aref" last="Alamri">Aref Alamri</name>
<name sortKey="Alotaibi, Sultan" sort="Alotaibi, Sultan" uniqKey="Alotaibi S" first="Sultan" last="Alotaibi">Sultan Alotaibi</name>
<name sortKey="Alzahrani, Fahd" sort="Alzahrani, Fahd" uniqKey="Alzahrani F" first="Fahd" last="Alzahrani">Fahd Alzahrani</name>
<name sortKey="Asiri, Mohammed O" sort="Asiri, Mohammed O" uniqKey="Asiri M" first="Mohammed O" last="Asiri">Mohammed O. Asiri</name>
<name sortKey="Hasan, Rami" sort="Hasan, Rami" uniqKey="Hasan R" first="Rami" last="Hasan">Rami Hasan</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005367 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005367 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:7079715 |texte= Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0 }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31271059" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |