Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0.
Identifieur interne : 000340 ( PubMed/Checkpoint ); précédent : 000339; suivant : 000341Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0.
Auteurs : Leonie-Sophie Hecht [Allemagne] ; Angeles Jurado-Jimenez [Allemagne] ; Markus Hess [Allemagne] ; Hussein El Halas [Allemagne] ; Gregor Bochenek [Allemagne] ; Hala Mohammed [Arabie saoudite] ; Fahd Alzahrani [Arabie saoudite] ; Mohammed O. Asiri [Arabie saoudite] ; Rami Hasan [Arabie saoudite] ; Aref Alamri [Arabie saoudite] ; Sultan Alotaibi [Arabie saoudite]Source :
- Future microbiology [ 1746-0921 ] ; 2019.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Arabie saoudite, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Protéines nucléocapside (génétique), Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines nucléocapside.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- Arabie saoudite, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Nucleocapsid Proteins (genetics), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction, Saudi Arabia, Sensitivity and Specificity.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, RNA, Viral.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques.
- virology : Coronavirus Infections.
- Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
Aim: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. Material & methods: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. Results: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. Conclusion: The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.
DOI: 10.2217/fmb-2019-0067
PubMed: 31271059
Affiliations:
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nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Jurado Jimenez, Angeles" sort="Jurado Jimenez, Angeles" uniqKey="Jurado Jimenez A" first="Angeles" last="Jurado-Jimenez">Angeles Jurado-Jimenez</name><affiliation wicri:level="3"><nlm:affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Hess, Markus" sort="Hess, Markus" uniqKey="Hess M" first="Markus" last="Hess">Markus Hess</name><affiliation wicri:level="3"><nlm:affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>aItona Diagnostics GmbH, Hamburg</wicri:regionArea><placeName><settlement type="city">Hambourg</settlement><region type="land" 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nuts="2">Hambourg</region></placeName></affiliation></author><author><name sortKey="Mohammed, Hala" sort="Mohammed, Hala" uniqKey="Mohammed H" first="Hala" last="Mohammed">Hala Mohammed</name><affiliation wicri:level="1"><nlm:affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alzahrani, Fahd" sort="Alzahrani, Fahd" uniqKey="Alzahrani F" first="Fahd" last="Alzahrani">Fahd Alzahrani</name><affiliation wicri:level="1"><nlm:affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Asiri, Mohammed O" sort="Asiri, Mohammed O" uniqKey="Asiri M" first="Mohammed O" last="Asiri">Mohammed O. Asiri</name><affiliation wicri:level="1"><nlm:affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Hasan, Rami" sort="Hasan, Rami" uniqKey="Hasan R" first="Rami" last="Hasan">Rami Hasan</name><affiliation wicri:level="1"><nlm:affiliation>King Fahad Medical City, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alamri, Aref" sort="Alamri, Aref" uniqKey="Alamri A" first="Aref" last="Alamri">Aref Alamri</name><affiliation wicri:level="1"><nlm:affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Ministry of Health, Riyadh Regional Laboratory, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alotaibi, Sultan" sort="Alotaibi, Sultan" uniqKey="Alotaibi S" first="Sultan" last="Alotaibi">Sultan Alotaibi</name><affiliation wicri:level="1"><nlm:affiliation>King Fahad Medical City, Riyadh, Saudi Arabia.</nlm:affiliation><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>King Fahad Medical City, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author></analytic><series><title level="j">Future microbiology</title><idno type="eISSN">1746-0921</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Nucleocapsid Proteins (genetics)</term><term>RNA, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction</term><term>Saudi Arabia</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Arabie saoudite</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Protéines nucléocapside (génétique)</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arabie saoudite</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><b>Aim:</b> We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. <b>Material & methods:</b> 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV <i>orf1a</i> and <i>upE</i>, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. <b>Results:</b> 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. <b>Conclusion:</b> The results suggest, that the RealStar<sup>®</sup> MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31271059</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1746-0921</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><PubDate><Year>2019</Year><Month>07</Month></PubDate></JournalIssue><Title>Future microbiology</Title><ISOAbbreviation>Future Microbiol</ISOAbbreviation></Journal><ArticleTitle>Verification and diagnostic evaluation of the RealStar<sup>®</sup> Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0.</ArticleTitle><Pagination><MedlinePgn>941-948</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2217/fmb-2019-0067</ELocationID><Abstract><AbstractText><b>Aim:</b> We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. <b>Material & methods:</b> 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV <i>orf1a</i> and <i>upE</i>, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. <b>Results:</b> 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. <b>Conclusion:</b> The results suggest, that the RealStar<sup>®</sup> MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hecht</LastName><ForeName>Leonie-Sophie</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jurado-Jimenez</LastName><ForeName>Angeles</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hess</LastName><ForeName>Markus</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Halas</LastName><ForeName>Hussein El</ForeName><Initials>HE</Initials><AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bochenek</LastName><ForeName>Gregor</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mohammed</LastName><ForeName>Hala</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alzahrani</LastName><ForeName>Fahd</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Asiri</LastName><ForeName>Mohammed O</ForeName><Initials>MO</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hasan</LastName><ForeName>Rami</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>King Fahad Medical City, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alamri</LastName><ForeName>Aref</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alotaibi</LastName><ForeName>Sultan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>King Fahad Medical City, Riyadh, Saudi Arabia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D023361">Validation Study</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Future Microbiol</MedlineTA><NlmUniqueID>101278120</NlmUniqueID><ISSNLinking>1746-0913</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="Y">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012529" MajorTopicYN="N" Type="Geographic">Saudi Arabia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">MERS-CoV</Keyword><Keyword MajorTopicYN="Y">WHO diagnostic scheme</Keyword><Keyword MajorTopicYN="Y">diagnostics</Keyword><Keyword MajorTopicYN="Y">emerging diseases</Keyword><Keyword MajorTopicYN="Y">molecular diagnostics</Keyword><Keyword 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J. 2015 Dec 22;12:222</Citation><ArticleIdList><ArticleId IdType="pubmed">26695637</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Saudi Med J. 2015 Apr;36(4):484-6</Citation><ArticleIdList><ArticleId IdType="pubmed">25828287</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1995-2005</Citation><ArticleIdList><ArticleId IdType="pubmed">12671061</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2017 Feb 9;376(6):584-594</Citation><ArticleIdList><ArticleId IdType="pubmed">28177862</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Korean J Intern Med. 2018 Mar;33(2):233-246</Citation><ArticleIdList><ArticleId IdType="pubmed">29506344</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>mBio. 2012 Nov 20;3(6):</Citation><ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>MMWR Morb Mortal Wkly Rep. 2003 Jun 13;52(23):547-50</Citation><ArticleIdList><ArticleId IdType="pubmed">12803194</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2015 Sep 5;386(9997):995-1007</Citation><ArticleIdList><ArticleId IdType="pubmed">26049252</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Euro Surveill. 2012 Dec 06;17(49):</Citation><ArticleIdList><ArticleId IdType="pubmed">23231891</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>F1000Res. 2017 Sep 1;6:1628</Citation><ArticleIdList><ArticleId IdType="pubmed">29026532</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Virol. 2014 Jun;60(2):168-71</Citation><ArticleIdList><ArticleId IdType="pubmed">24726679</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Microbiol Rev. 2015 Apr;28(2):465-522</Citation><ArticleIdList><ArticleId IdType="pubmed">25810418</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2014 Oct;88(19):11297-303</Citation><ArticleIdList><ArticleId IdType="pubmed">25031349</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Euro Surveill. 2012 Sep 27;17(39):</Citation><ArticleIdList><ArticleId IdType="pubmed">23041020</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>PLoS One. 2017 Sep 19;12(9):e0184692</Citation><ArticleIdList><ArticleId IdType="pubmed">28926588</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Can Commun Dis Rep. 2017 Oct 05;43(10):206-211</Citation><ArticleIdList><ArticleId IdType="pubmed">29770047</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Arabie saoudite</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><country name="Allemagne"><region name="Hambourg"><name sortKey="Hecht, Leonie Sophie" sort="Hecht, Leonie Sophie" uniqKey="Hecht L" first="Leonie-Sophie" last="Hecht">Leonie-Sophie Hecht</name></region><name sortKey="Bochenek, Gregor" sort="Bochenek, Gregor" uniqKey="Bochenek G" first="Gregor" last="Bochenek">Gregor Bochenek</name><name sortKey="Halas, Hussein El" sort="Halas, Hussein El" uniqKey="Halas H" first="Hussein El" last="Halas">Hussein El Halas</name><name sortKey="Hess, Markus" sort="Hess, Markus" uniqKey="Hess M" first="Markus" last="Hess">Markus Hess</name><name sortKey="Jurado Jimenez, Angeles" sort="Jurado Jimenez, Angeles" uniqKey="Jurado Jimenez A" first="Angeles" last="Jurado-Jimenez">Angeles 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