Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0.
Identifieur interne : 000490 ( PubMed/Curation ); précédent : 000489; suivant : 000491Verification and diagnostic evaluation of the RealStar® Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0.
Auteurs : Leonie-Sophie Hecht [Allemagne] ; Angeles Jurado-Jimenez [Allemagne] ; Markus Hess [Allemagne] ; Hussein El Halas [Allemagne] ; Gregor Bochenek [Allemagne] ; Hala Mohammed [Arabie saoudite] ; Fahd Alzahrani [Arabie saoudite] ; Mohammed O. Asiri [Arabie saoudite] ; Rami Hasan [Arabie saoudite] ; Aref Alamri [Arabie saoudite] ; Sultan Alotaibi [Arabie saoudite]Source :
- Future microbiology [ 1746-0921 ] ; 2019.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Arabie saoudite, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Protéines nucléocapside (génétique), Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient, Protéines nucléocapside.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Infections à coronavirus.
- Arabie saoudite, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Nucleocapsid Proteins (genetics), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction, Saudi Arabia, Sensitivity and Specificity.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, RNA, Viral.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques.
- virology : Coronavirus Infections.
- Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
Aim: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. Material & methods: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. Results: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. Conclusion: The results suggest, that the RealStar® MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.
DOI: 10.2217/fmb-2019-0067
PubMed: 31271059
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pubmed:31271059Le document en format XML
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<front><div type="abstract" xml:lang="en"><b>Aim:</b>
We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. <b>Material & methods:</b>
33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV <i>orf1a</i>
and <i>upE</i>
, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. <b>Results:</b>
18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. <b>Conclusion:</b>
The results suggest, that the RealStar<sup>®</sup>
MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.</div>
</front>
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<Month>02</Month>
<Day>04</Day>
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<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>19</Day>
</DateRevised>
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<JournalIssue CitedMedium="Internet"><Volume>14</Volume>
<PubDate><Year>2019</Year>
<Month>07</Month>
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<Title>Future microbiology</Title>
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<ArticleTitle>Verification and diagnostic evaluation of the RealStar<sup>®</sup>
Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0.</ArticleTitle>
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</Pagination>
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<Abstract><AbstractText><b>Aim:</b>
We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. <b>Material & methods:</b>
33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV <i>orf1a</i>
and <i>upE</i>
, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. <b>Results:</b>
18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. <b>Conclusion:</b>
The results suggest, that the RealStar<sup>®</sup>
MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hecht</LastName>
<ForeName>Leonie-Sophie</ForeName>
<Initials>LS</Initials>
<AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jurado-Jimenez</LastName>
<ForeName>Angeles</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hess</LastName>
<ForeName>Markus</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Halas</LastName>
<ForeName>Hussein El</ForeName>
<Initials>HE</Initials>
<AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Bochenek</LastName>
<ForeName>Gregor</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>aItona Diagnostics GmbH, Hamburg, Germany.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Mohammed</LastName>
<ForeName>Hala</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
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<AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
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<Initials>MO</Initials>
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</AffiliationInfo>
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<Initials>R</Initials>
<AffiliationInfo><Affiliation>King Fahad Medical City, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Alamri</LastName>
<ForeName>Aref</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Ministry of Health, Riyadh Regional Laboratory, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Alotaibi</LastName>
<ForeName>Sultan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>King Fahad Medical City, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
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<Month>07</Month>
<Day>04</Day>
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