Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction
Identifieur interne : 003919 ( Main/Exploration ); précédent : 003918; suivant : 003920Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction
Auteurs : T. Nishiura [Japon] ; K. Abe [Japon]Source :
- Archives of Oral Biology [ 0003-9969 ] ; 1999.
English descriptors
- KwdEn :
- Cystatin, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Gene-expression, Quantitative reverse transcription-polymerase chain reaction (RT-PCR), RT-PCR, reverse transcription-polymerase chain reaction, Submandibular gland, TIMP, tissue inhibitor of metalloproteinase, Tissue inhibitor of metalloproteinase, dNTP, deoxynucleoside triphosphate.
- Teeft :
- Acinar cells, Band intensities, Biochem, Biol, Cdna, Cell growth, Chain reaction, Cleft formation, Collagenase, Competitor, Competitor construction, Cystatin, Cystatins, Cysteine, Cysteine proteinases, Extracellular matrix, G3pdh, G3pdh mrnas, Gene expression, Gingival, Gingival crevicular, Gingival tissues, Gland, Hayakawa, Henskens, Housekeeping gene, Human cystatin, Image analysis, Inhibitor, Matrix, Matrix metalloproteinases, Metalloproteinases, Molar, Molar ratio, Mrna, Nishiura, Oral biology, Parotid saliva, Periodontal, Periodontal treatment, Periodontitis, Postnatal, Postnatal changes, Postnatal development, Primer, Proteinase, Proteinase inhibitors, Saliva, Salivary, Salivary glands, Submandibular, Submandibular gland, Submandibular glands, Submandibular saliva, Submaxillary gland, Target cdna, Terminal tubule cells, Tissue inhibitor, Tissue inhibitors, Tubule, Whole saliva.
Abstract
Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.
Url:
DOI: 10.1016/S0003-9969(98)00096-X
Affiliations:
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Abe</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:74F0EFDC679AFAB1305B3D048189E7A6C3CBA827</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0003-9969(98)00096-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002109</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002109</idno><idno type="wicri:Area/Istex/Curation">002109</idno><idno type="wicri:Area/Istex/Checkpoint">001172</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001172</idno><idno type="wicri:doubleKey">0003-9969:1999:Nishiura T:postnatal:changes:of</idno><idno type="wicri:Area/Main/Merge">003964</idno><idno type="wicri:Area/Main/Curation">003919</idno><idno type="wicri:Area/Main/Exploration">003919</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title><author><name sortKey="Nishiura, T" sort="Nishiura, T" uniqKey="Nishiura T" first="T" last="Nishiura">T. Nishiura</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193</wicri:regionArea><wicri:noRegion>Fukuoka 814-0193</wicri:noRegion></affiliation></author><author><name sortKey="Abe, K" sort="Abe, K" uniqKey="Abe K" first="K" last="Abe">K. Abe</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193</wicri:regionArea><wicri:noRegion>Fukuoka 814-0193</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Oral Biology</title><title level="j" type="abbrev">AOB</title><idno type="ISSN">0003-9969</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">44</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="15">15</biblScope><biblScope unit="page" to="26">26</biblScope></imprint><idno type="ISSN">0003-9969</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-9969</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cystatin</term><term>G3PDH, glyceraldehyde-3-phosphate dehydrogenase</term><term>Gene-expression</term><term>Quantitative reverse transcription-polymerase chain reaction (RT-PCR)</term><term>RT-PCR, reverse transcription-polymerase chain reaction</term><term>Submandibular gland</term><term>TIMP, tissue inhibitor of metalloproteinase</term><term>Tissue inhibitor of metalloproteinase</term><term>dNTP, deoxynucleoside triphosphate</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acinar cells</term><term>Band intensities</term><term>Biochem</term><term>Biol</term><term>Cdna</term><term>Cell growth</term><term>Chain reaction</term><term>Cleft formation</term><term>Collagenase</term><term>Competitor</term><term>Competitor construction</term><term>Cystatin</term><term>Cystatins</term><term>Cysteine</term><term>Cysteine proteinases</term><term>Extracellular matrix</term><term>G3pdh</term><term>G3pdh mrnas</term><term>Gene expression</term><term>Gingival</term><term>Gingival crevicular</term><term>Gingival tissues</term><term>Gland</term><term>Hayakawa</term><term>Henskens</term><term>Housekeeping gene</term><term>Human cystatin</term><term>Image analysis</term><term>Inhibitor</term><term>Matrix</term><term>Matrix metalloproteinases</term><term>Metalloproteinases</term><term>Molar</term><term>Molar ratio</term><term>Mrna</term><term>Nishiura</term><term>Oral biology</term><term>Parotid saliva</term><term>Periodontal</term><term>Periodontal treatment</term><term>Periodontitis</term><term>Postnatal</term><term>Postnatal changes</term><term>Postnatal development</term><term>Primer</term><term>Proteinase</term><term>Proteinase inhibitors</term><term>Saliva</term><term>Salivary</term><term>Salivary glands</term><term>Submandibular</term><term>Submandibular gland</term><term>Submandibular glands</term><term>Submandibular saliva</term><term>Submaxillary gland</term><term>Target cdna</term><term>Terminal tubule cells</term><term>Tissue inhibitor</term><term>Tissue inhibitors</term><term>Tubule</term><term>Whole saliva</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Nishiura, T" sort="Nishiura, T" uniqKey="Nishiura T" first="T" last="Nishiura">T. Nishiura</name></noRegion><name sortKey="Abe, K" sort="Abe, K" uniqKey="Abe K" first="K" last="Abe">K. Abe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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