Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction
Identifieur interne : 002109 ( Istex/Corpus ); précédent : 002108; suivant : 002110Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction
Auteurs : T. Nishiura ; K. AbeSource :
- Archives of Oral Biology [ 0003-9969 ] ; 1999.
English descriptors
- KwdEn :
- Cystatin, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Gene-expression, Quantitative reverse transcription-polymerase chain reaction (RT-PCR), RT-PCR, reverse transcription-polymerase chain reaction, Submandibular gland, TIMP, tissue inhibitor of metalloproteinase, Tissue inhibitor of metalloproteinase, dNTP, deoxynucleoside triphosphate.
- Teeft :
- Acinar cells, Band intensities, Biochem, Biol, Cdna, Cell growth, Chain reaction, Cleft formation, Collagenase, Competitor, Competitor construction, Cystatin, Cystatins, Cysteine, Cysteine proteinases, Extracellular matrix, G3pdh, G3pdh mrnas, Gene expression, Gingival, Gingival crevicular, Gingival tissues, Gland, Hayakawa, Henskens, Housekeeping gene, Human cystatin, Image analysis, Inhibitor, Matrix, Matrix metalloproteinases, Metalloproteinases, Molar, Molar ratio, Mrna, Nishiura, Oral biology, Parotid saliva, Periodontal, Periodontal treatment, Periodontitis, Postnatal, Postnatal changes, Postnatal development, Primer, Proteinase, Proteinase inhibitors, Saliva, Salivary, Salivary glands, Submandibular, Submandibular gland, Submandibular glands, Submandibular saliva, Submaxillary gland, Target cdna, Terminal tubule cells, Tissue inhibitor, Tissue inhibitors, Tubule, Whole saliva.
Abstract
Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.
Url:
DOI: 10.1016/S0003-9969(98)00096-X
Links to Exploration step
ISTEX:74F0EFDC679AFAB1305B3D048189E7A6C3CBA827Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title><author><name sortKey="Nishiura, T" sort="Nishiura, T" uniqKey="Nishiura T" first="T" last="Nishiura">T. Nishiura</name><affiliation><mods:affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</mods:affiliation></affiliation></author><author><name sortKey="Abe, K" sort="Abe, K" uniqKey="Abe K" first="K" last="Abe">K. Abe</name><affiliation><mods:affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:74F0EFDC679AFAB1305B3D048189E7A6C3CBA827</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0003-9969(98)00096-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002109</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002109</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title><author><name sortKey="Nishiura, T" sort="Nishiura, T" uniqKey="Nishiura T" first="T" last="Nishiura">T. Nishiura</name><affiliation><mods:affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</mods:affiliation></affiliation></author><author><name sortKey="Abe, K" sort="Abe, K" uniqKey="Abe K" first="K" last="Abe">K. Abe</name><affiliation><mods:affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Oral Biology</title><title level="j" type="abbrev">AOB</title><idno type="ISSN">0003-9969</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">44</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="15">15</biblScope><biblScope unit="page" to="26">26</biblScope></imprint><idno type="ISSN">0003-9969</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-9969</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cystatin</term><term>G3PDH, glyceraldehyde-3-phosphate dehydrogenase</term><term>Gene-expression</term><term>Quantitative reverse transcription-polymerase chain reaction (RT-PCR)</term><term>RT-PCR, reverse transcription-polymerase chain reaction</term><term>Submandibular gland</term><term>TIMP, tissue inhibitor of metalloproteinase</term><term>Tissue inhibitor of metalloproteinase</term><term>dNTP, deoxynucleoside triphosphate</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acinar cells</term><term>Band intensities</term><term>Biochem</term><term>Biol</term><term>Cdna</term><term>Cell growth</term><term>Chain reaction</term><term>Cleft formation</term><term>Collagenase</term><term>Competitor</term><term>Competitor construction</term><term>Cystatin</term><term>Cystatins</term><term>Cysteine</term><term>Cysteine proteinases</term><term>Extracellular matrix</term><term>G3pdh</term><term>G3pdh mrnas</term><term>Gene expression</term><term>Gingival</term><term>Gingival crevicular</term><term>Gingival tissues</term><term>Gland</term><term>Hayakawa</term><term>Henskens</term><term>Housekeeping gene</term><term>Human cystatin</term><term>Image analysis</term><term>Inhibitor</term><term>Matrix</term><term>Matrix metalloproteinases</term><term>Metalloproteinases</term><term>Molar</term><term>Molar ratio</term><term>Mrna</term><term>Nishiura</term><term>Oral biology</term><term>Parotid saliva</term><term>Periodontal</term><term>Periodontal treatment</term><term>Periodontitis</term><term>Postnatal</term><term>Postnatal changes</term><term>Postnatal development</term><term>Primer</term><term>Proteinase</term><term>Proteinase inhibitors</term><term>Saliva</term><term>Salivary</term><term>Salivary glands</term><term>Submandibular</term><term>Submandibular gland</term><term>Submandibular glands</term><term>Submandibular saliva</term><term>Submaxillary gland</term><term>Target cdna</term><term>Terminal tubule cells</term><term>Tissue inhibitor</term><term>Tissue inhibitors</term><term>Tubule</term><term>Whole saliva</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>cystatin</json:string><json:string>mrna</json:string><json:string>submandibular</json:string><json:string>g3pdh</json:string><json:string>cystatins</json:string><json:string>cdna</json:string><json:string>submandibular gland</json:string><json:string>nishiura</json:string><json:string>primer</json:string><json:string>proteinase</json:string><json:string>metalloproteinases</json:string><json:string>postnatal</json:string><json:string>submandibular glands</json:string><json:string>biol</json:string><json:string>matrix metalloproteinases</json:string><json:string>oral biology</json:string><json:string>tubule</json:string><json:string>cysteine</json:string><json:string>hayakawa</json:string><json:string>matrix</json:string><json:string>collagenase</json:string><json:string>inhibitor</json:string><json:string>periodontal</json:string><json:string>gingival</json:string><json:string>henskens</json:string><json:string>periodontitis</json:string><json:string>tissue inhibitor</json:string><json:string>biochem</json:string><json:string>competitor</json:string><json:string>submandibular saliva</json:string><json:string>postnatal development</json:string><json:string>image analysis</json:string><json:string>cysteine proteinases</json:string><json:string>salivary</json:string><json:string>whole saliva</json:string><json:string>tissue inhibitors</json:string><json:string>postnatal changes</json:string><json:string>gingival crevicular</json:string><json:string>salivary glands</json:string><json:string>extracellular matrix</json:string><json:string>competitor construction</json:string><json:string>band intensities</json:string><json:string>terminal tubule cells</json:string><json:string>molar</json:string><json:string>submaxillary gland</json:string><json:string>g3pdh mrnas</json:string><json:string>target cdna</json:string><json:string>cell growth</json:string><json:string>housekeeping gene</json:string><json:string>gene expression</json:string><json:string>gingival tissues</json:string><json:string>chain reaction</json:string><json:string>molar ratio</json:string><json:string>cleft formation</json:string><json:string>acinar cells</json:string><json:string>periodontal treatment</json:string><json:string>parotid saliva</json:string><json:string>human cystatin</json:string><json:string>proteinase inhibitors</json:string><json:string>gland</json:string><json:string>saliva</json:string></teeft></keywords><author><json:item><name>T Nishiura</name><affiliations><json:string>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</json:string></affiliations></json:item><json:item><name>K Abe</name><affiliations><json:string>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Tissue inhibitor of metalloproteinase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cystatin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Quantitative reverse transcription-polymerase chain reaction (RT-PCR)</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Submandibular gland</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Gene-expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>dNTP, deoxynucleoside triphosphate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>G3PDH, glyceraldehyde-3-phosphate dehydrogenase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RT-PCR, reverse transcription-polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TIMP, tissue inhibitor of metalloproteinase</value></json:item></subject><articleId><json:string>759</json:string></articleId><arkIstex>ark:/67375/6H6-0D880H49-Z</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.</abstract><qualityIndicators><score>9.004</score><pdfWordCount>5641</pdfWordCount><pdfCharCount>35152</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>595 x 842 pts (A4)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>167</abstractWordCount><abstractCharCount>1194</abstractCharCount><keywordCount>9</keywordCount></qualityIndicators><title>Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title><pmid><json:string>10075146</json:string></pmid><pii><json:string>S0003-9969(98)00096-X</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Archives of Oral Biology</title><language><json:string>unknown</json:string></language><publicationDate>1999</publicationDate><issn><json:string>0003-9969</json:string></issn><pii><json:string>S0003-9969(00)X0045-3</json:string></pii><volume>44</volume><issue>1</issue><pages><first>15</first><last>26</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1999</json:string><json:string>2-15-1</json:string></date><geogName></geogName><orgName><json:string>Lifetech Oriental Co.</json:string><json:string>Hitach Software Engineering Co., Yokohama, Japan</json:string><json:string>Elsevier Science Ltd.</json:string><json:string>Clontech</json:string><json:string>Japan Accepted</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>T. Nishiura</json:string><json:string>C. Means</json:string><json:string>K. Abe</json:string></persName><placeName><json:string>Japan</json:string><json:string>Tokyo</json:string><json:string>Osaka</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Aguirre et al. (1992)</json:string><json:string>Tavera et al., 1990</json:string><json:string>Kubota et al., 1996</json:string><json:string>Murphy et al.</json:string><json:string>Okada et al., 1994</json:string><json:string>Gresik, 1980</json:string><json:string>Ito et al., 1975</json:string><json:string>Drouin et al., 1988</json:string><json:string>Shaw et al. (1990)</json:string><json:string>Bedi, 1991</json:string><json:string>Abrahamson et al. 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(1997)</json:string><json:string>Nishiura et al., 1991</json:string><json:string>Nakanishi et al., 1988</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-0D880H49-Z</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - dentistry, oral surgery & medicine</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - dentistry</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Cell Biology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Dentistry</json:string><json:string>3 - General Dentistry</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Otorhinolaryngology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - pathologie osteoarticulaire</json:string></inist></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0003-9969(98)00096-X</json:string></doi><id>74F0EFDC679AFAB1305B3D048189E7A6C3CBA827</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1999 Elsevier Science Ltd</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1999</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Fig. 1: RT-PCR analysis of the expression of TIMP-1, TIMP-2, cystatin S, cystatin C and G3PDH mRNAs in rat submandibular glands and the limited digestion of the amplified DNA products with restriction enzymes. The RT-PCR was done in the absence (−) or presence (+) of reverse transcriptase. Blank, reaction performed without RNA. The amplified DNA product was digested with the indicated enzyme and the expected band sizes were obtained.</note><note type="content">Fig. 2: Kinetics of amplification of TIMP-1 (A), TIMP-2 (B), cystatin S (C), cystatin C (D) and G3PHD (E) cDNAs and their competitors, and the calibration standard curve (F). Approximately equal molar quantities of a target cDNA and its competitor were coamplified in a single PCR tube at various cycle numbers and separated on agarose gel. The band intensities were determined by image analysis.</note><note type="content">Fig. 3: Representative composite data for competitive RT-PCR of TIMP-1, TIMP-2, cystatin S, cystatin C and G3PDH mRNAs. The product sizes are the same as listed in Table 1. Two-fold serial dilutions of the competitor from right to left were coamplified with constant amounts of target cDNA. For TIMP-1, lanes A to G correspond to 7.5, 15, 30, 60, 120, 240, 480 molecules of the competitor. For TIMP-2, lanes A to G correspond to (4.7, 9.4, 18.8, 37.5, 75, 150, 300)×102 molecules of the competitor. For cystatin S, lanes A to I correspond to 2.93, 5.86, 11.7, 23.4, 47, 94, 188, 375, 750 molecules of the competitor. For cystatin C, lanes A to E correspond to (4.69, 9.4, 18.8, 37.5, 75)×102 molecules of the competitor. For G3PDH, lanes A to E correspond to (3,6, 12, 24, 48)×103 molecules of the competitor. Cycle numbers of PCR are shown on the right-hand side of the electrophoretograms.</note><note type="content">Fig. 4: Log-log expression of the data shown in Fig. 3 for TIMP-1 in submandibular gland of rats 1 week of age. The y-axis represents the log of the molar ratio of amplified TIMP-1 and its competitor. The x-axis represents the log of the number of molecules of competitor present in each reaction. The equivalence point is observed at the zero point on the y-axis.</note><note type="content">Fig. 5: Relative expression of TIMP-1 and TIMP-2 mRNA in rat submandibular gland during postnatal development: (A) TIMP-1 mRNA relative to G3PHD; (B) TIMP-2 mRNA relative to G3PDH; (C) TIMP-1 mRNA relative to TIMP-2; (D) TIMP-2 mRNA relative to TIMP-1. All values are expressed as mean±SEM percentage of transcript concentrations relative to G3PDH, TIMP-1 or TIMP-2. Means with different letters are significantly different by the Fisher test (p<0.05). NS, not significant (p>0.05) by ANOVA.</note><note type="content">Fig. 6: Relative expression of cystatin S and cystatin C mRNAs in rat submandibular gland during postnatal development: (A) cystatin S mRNA relative to G3PDH; (B) cystatin C mRNA relative to G3PDH; (C) cystatin S mRNA relative to cystatin C; (D) cystatin C mRNA relative to cystatin S. All values are expressed as mean±SEM percentage of transcript concentrations relative to G3PDH, cystatin S or cystatin C. Means with different letters are significantly different by the Fisher test (p<0.05). NS, not significant (p>0.05) by ANOVA.</note><note type="content">Table 1: Sequences, lengths and cDNA localization of oligonucleotide primers, and PCR product size of cellular mRNA and competitor</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title><author xml:id="author-0000"><persName><forename type="first">T</forename><surname>Nishiura</surname></persName><note type="correspondence"><p>Corresponding author. Tel.: 81-92-801-0411 (Ex. 670); fax: 81-92-801-4909</p></note><affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</affiliation></author><author xml:id="author-0001"><persName><forename type="first">K</forename><surname>Abe</surname></persName><affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</affiliation></author><idno type="istex">74F0EFDC679AFAB1305B3D048189E7A6C3CBA827</idno><idno type="ark">ark:/67375/6H6-0D880H49-Z</idno><idno type="DOI">10.1016/S0003-9969(98)00096-X</idno><idno type="PII">S0003-9969(98)00096-X</idno><idno type="article-id">759</idno></analytic><monogr><title level="j">Archives of Oral Biology</title><title level="j" type="abbrev">AOB</title><idno type="pISSN">0003-9969</idno><idno type="PII">S0003-9969(00)X0045-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">44</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="15">15</biblScope><biblScope unit="page" to="26">26</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Tissue inhibitor of metalloproteinase</term></item><item><term>Cystatin</term></item><item><term>Quantitative reverse transcription-polymerase chain reaction (RT-PCR)</term></item><item><term>Submandibular gland</term></item><item><term>Gene-expression</term></item></list></keywords></textClass><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>dNTP, deoxynucleoside triphosphate</term></item><item><term>G3PDH, glyceraldehyde-3-phosphate dehydrogenase</term></item><item><term>RT-PCR, reverse transcription-polymerase chain reaction</term></item><item><term>TIMP, tissue inhibitor of metalloproteinase</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity><istex:entity SYSTEM="gr6" NDATA="IMAGE" name="gr6"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>AOB</jid><aid>759</aid><ce:pii>S0003-9969(98)00096-X</ce:pii><ce:doi>10.1016/S0003-9969(98)00096-X</ce:doi><ce:copyright year="1999" type="full-transfer">Elsevier Science Ltd</ce:copyright></item-info><head><ce:title>Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</ce:title><ce:author-group><ce:author><ce:given-name>T</ce:given-name><ce:surname>Nishiura</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>K</ce:given-name><ce:surname>Abe</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: 81-92-801-0411 (Ex. 670); fax: 81-92-801-4909</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="9" month="9" year="1998"></ce:date-accepted><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0045">The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Tissue inhibitor of metalloproteinase</ce:text></ce:keyword><ce:keyword><ce:text>Cystatin</ce:text></ce:keyword><ce:keyword><ce:text>Quantitative reverse transcription-polymerase chain reaction (RT-PCR)</ce:text></ce:keyword><ce:keyword><ce:text>Submandibular gland</ce:text></ce:keyword><ce:keyword><ce:text>Gene-expression</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>dNTP, deoxynucleoside triphosphate</ce:text></ce:keyword><ce:keyword><ce:text>G3PDH, glyceraldehyde-3-phosphate dehydrogenase</ce:text></ce:keyword><ce:keyword><ce:text>RT-PCR, reverse transcription-polymerase chain reaction</ce:text></ce:keyword><ce:keyword><ce:text>TIMP, tissue inhibitor of metalloproteinase</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Postnatal changes of gene expression for tissue inhibitors of metalloproteinase-1 and -2 and cystatins S and C, in rat submandibular gland demonstrated by quantitative reverse transcription-polymerase chain reaction</title></titleInfo><name type="personal"><namePart type="given">T</namePart><namePart type="family">Nishiura</namePart><affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</affiliation><description>Corresponding author. Tel.: 81-92-801-0411 (Ex. 670); fax: 81-92-801-4909</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">Abe</namePart><affiliation>Department of Oral Biochemistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The rat submandibular gland is not fully developed at birth and definitive differentiation takes place postnatally. The steady-state mRNA expression for the four proteinase inhibitor molecules, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and cystatins S and C, and for a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), in rat submandibular glands was measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) at different stages of postnatal development. The gene-expression patterns of TIMP-1 and -2 relative to G3PDH were similar to each other. The TIMP-2 and cystatin C genes were more highly expressed than those of TIMP-1 and cystatin S at all stages. Moreover, the gene expressions of TIMP-1 and -2, and of cystatins S and C, were predominant between 1 and 7, and 7 and 12 weeks of age, respectively, and coincided developmentally with the regression of terminal tubule cells and the differentiation of granular convoluted tubule cells, respectively. Quantitative competitive RT-PCR allowed accurate measurement of small changes in the steady-state concentrations of these proteinase-inhibitor mRNA molecules.</abstract><note type="content">Fig. 1: RT-PCR analysis of the expression of TIMP-1, TIMP-2, cystatin S, cystatin C and G3PDH mRNAs in rat submandibular glands and the limited digestion of the amplified DNA products with restriction enzymes. The RT-PCR was done in the absence (−) or presence (+) of reverse transcriptase. Blank, reaction performed without RNA. The amplified DNA product was digested with the indicated enzyme and the expected band sizes were obtained.</note><note type="content">Fig. 2: Kinetics of amplification of TIMP-1 (A), TIMP-2 (B), cystatin S (C), cystatin C (D) and G3PHD (E) cDNAs and their competitors, and the calibration standard curve (F). Approximately equal molar quantities of a target cDNA and its competitor were coamplified in a single PCR tube at various cycle numbers and separated on agarose gel. The band intensities were determined by image analysis.</note><note type="content">Fig. 3: Representative composite data for competitive RT-PCR of TIMP-1, TIMP-2, cystatin S, cystatin C and G3PDH mRNAs. The product sizes are the same as listed in Table 1. Two-fold serial dilutions of the competitor from right to left were coamplified with constant amounts of target cDNA. For TIMP-1, lanes A to G correspond to 7.5, 15, 30, 60, 120, 240, 480 molecules of the competitor. For TIMP-2, lanes A to G correspond to (4.7, 9.4, 18.8, 37.5, 75, 150, 300)×102 molecules of the competitor. For cystatin S, lanes A to I correspond to 2.93, 5.86, 11.7, 23.4, 47, 94, 188, 375, 750 molecules of the competitor. For cystatin C, lanes A to E correspond to (4.69, 9.4, 18.8, 37.5, 75)×102 molecules of the competitor. For G3PDH, lanes A to E correspond to (3,6, 12, 24, 48)×103 molecules of the competitor. Cycle numbers of PCR are shown on the right-hand side of the electrophoretograms.</note><note type="content">Fig. 4: Log-log expression of the data shown in Fig. 3 for TIMP-1 in submandibular gland of rats 1 week of age. The y-axis represents the log of the molar ratio of amplified TIMP-1 and its competitor. The x-axis represents the log of the number of molecules of competitor present in each reaction. The equivalence point is observed at the zero point on the y-axis.</note><note type="content">Fig. 5: Relative expression of TIMP-1 and TIMP-2 mRNA in rat submandibular gland during postnatal development: (A) TIMP-1 mRNA relative to G3PHD; (B) TIMP-2 mRNA relative to G3PDH; (C) TIMP-1 mRNA relative to TIMP-2; (D) TIMP-2 mRNA relative to TIMP-1. All values are expressed as mean±SEM percentage of transcript concentrations relative to G3PDH, TIMP-1 or TIMP-2. Means with different letters are significantly different by the Fisher test (p<0.05). NS, not significant (p>0.05) by ANOVA.</note><note type="content">Fig. 6: Relative expression of cystatin S and cystatin C mRNAs in rat submandibular gland during postnatal development: (A) cystatin S mRNA relative to G3PDH; (B) cystatin C mRNA relative to G3PDH; (C) cystatin S mRNA relative to cystatin C; (D) cystatin C mRNA relative to cystatin S. All values are expressed as mean±SEM percentage of transcript concentrations relative to G3PDH, cystatin S or cystatin C. Means with different letters are significantly different by the Fisher test (p<0.05). NS, not significant (p>0.05) by ANOVA.</note><note type="content">Table 1: Sequences, lengths and cDNA localization of oligonucleotide primers, and PCR product size of cellular mRNA and competitor</note><subject lang="en"><genre>Keywords</genre><topic>Tissue inhibitor of metalloproteinase</topic><topic>Cystatin</topic><topic>Quantitative reverse transcription-polymerase chain reaction (RT-PCR)</topic><topic>Submandibular gland</topic><topic>Gene-expression</topic></subject><subject lang="en"><genre>Abbreviations</genre><topic>dNTP, deoxynucleoside triphosphate</topic><topic>G3PDH, glyceraldehyde-3-phosphate dehydrogenase</topic><topic>RT-PCR, reverse transcription-polymerase chain reaction</topic><topic>TIMP, tissue inhibitor of metalloproteinase</topic></subject><relatedItem type="host"><titleInfo><title>Archives of Oral Biology</title></titleInfo><titleInfo type="abbreviated"><title>AOB</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued></originInfo><identifier type="ISSN">0003-9969</identifier><identifier type="PII">S0003-9969(00)X0045-3</identifier><part><date>1999</date><detail type="volume"><number>44</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>96</end></extent><extent unit="pages"><start>15</start><end>26</end></extent></part></relatedItem><identifier type="istex">74F0EFDC679AFAB1305B3D048189E7A6C3CBA827</identifier><identifier type="ark">ark:/67375/6H6-0D880H49-Z</identifier><identifier type="DOI">10.1016/S0003-9969(98)00096-X</identifier><identifier type="PII">S0003-9969(98)00096-X</identifier><identifier type="ArticleID">759</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science Ltd</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier Science Ltd, ©1999</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-0D880H49-Z/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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