MersV1, Main, Exploration, bibRecord, 004C46

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos

Identifieur interne : 004C46 ( Main/Exploration ); précédent : 004C45; suivant : 004C47

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos

Auteurs : John Shuttleworth [Royaume-Uni] ; Glenn Matthews [Royaume-Uni] ; Les Dale [Royaume-Uni] ; Chris Baker [Royaume-Uni] ; Alan Colman [Royaume-Uni]

Source :

Gene [ 0378-1119 ] ; 1988.

RBID : ISTEX:A3CFC958B8DFCFB0048039E428F89D0AF0C564C2

Descripteurs français

KwdFr :
- ARN (génétique), ARN antisens, ARN messager (antagonistes et inhibiteurs), ARN messager (génétique), Animaux, Cinétique, Embryon non mammalien (métabolisme), Femelle, Fécondation, Histone (génétique), Ovocytes (métabolisme), Sondes oligonucléotidiques, Xenopus.
MESH :
- antagonistes et inhibiteurs : ARN messager.
- génétique : ARN, ARN messager, Histone.
- métabolisme : Embryon non mammalien, Ovocytes.
- ARN antisens, Animaux, Cinétique, Femelle, Fécondation, Sondes oligonucléotidiques, Xenopus.

English descriptors

KwdEn :
- Animals, Embryo, Nonmammalian (metabolism), Female, Fertilization, Histones (genetics), Kinetics, Oligonucleotide Probes, Oocytes (metabolism), RNA (genetics), RNA, Antisense, RNA, Messenger (antagonists & inhibitors), RNA, Messenger (genetics), Xenopus.
MESH :
- chemical , antagonists & inhibitors : RNA, Messenger.
- chemical , genetics : Histones, RNA, RNA, Messenger.
- metabolism : Embryo, Nonmammalian, Oocytes.
Teeft :
- Animals, Antisense, Antisense oligo, Antisense oligos, Cazenave, Cleavage, Embryo, Female, Fertilised, Fertilised embryos, Fertilization, Further cleavage, Histone, Kinetics, Maternal mrna, Melton, Mrna, Mrna cleavage, Mrna levels, Nucleic, Nucleic acids, Oligo, Oligo injection, Oligonucleotide Probes, Oligos, Oocyte, Proc, Protein synthesis, RNA, Antisense, Rnase cleavage, Second injection, Shuttleworth, Small proportion, Unfertihsed eggs, Unfertilised, Unfertilised eggs, Vegetal, Vegetal pole, Xenopus, Xenopus oocytes.

Abstract

Abstract: We have investigated the effect of specific antisense oligodeoxynucleotides (oligos) on endogenous histone H4 mRNA in Xenopus oocytes, eggs and embryos. In unfertilised eggs and non-matured oocytes, one 20-mer oligo (H4-1) mediated the RNAse H-like cleavage of up to 95% of H4 mRNA (which included polysomal mRNA), and cleavage was still obtained when the size of the oligo was reduced to a 10-mer; no cleavage was observed with 6- and 8-mers. The residual uncleaved mRNA appeared to be completely inaccessible to H4-1 since a second injection caused no further cleavage. A second 20-mer (H4-2) directed against a different region of H4 mRNA was much less effective (<5% cleavage). In fertilised embryos, injections of H4-1 and an oligo directed against the localised Vg1 mRNA caused less cleavage than in oocytes and also showed signs of inducing localised, non-specific mRNA cleavage. However we have been able to prepare fertilised embryos devoid of Vgl mRNA by maturing and fertilising oligo-injected oocytes in vitro.

Url:

https://api.istex.fr/ark:/67375/6H6-9DQRZVZC-2/fulltext.pdf

DOI: 10.1016/0378-1119(88)90152-7

Affiliations:

Le document en format XML

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<term>Fertilization</term>
<term>Histones (genetics)</term>
<term>Kinetics</term>
<term>Oligonucleotide Probes</term>
<term>Oocytes (metabolism)</term>
<term>RNA (genetics)</term>
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<term>RNA, Messenger (antagonists & inhibitors)</term>
<term>RNA, Messenger (genetics)</term>
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<term>ARN messager (génétique)</term>
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<term>Cinétique</term>
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<term>Fécondation</term>
<term>Histone (génétique)</term>
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<term>Sondes oligonucléotidiques</term>
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<term>RNA</term>
<term>RNA, Messenger</term>
</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN</term>
<term>ARN messager</term>
<term>Histone</term>
</keywords>
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<term>Kinetics</term>
<term>Maternal mrna</term>
<term>Melton</term>
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<term>Mrna cleavage</term>
<term>Mrna levels</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Oligo</term>
<term>Oligo injection</term>
<term>Oligonucleotide Probes</term>
<term>Oligos</term>
<term>Oocyte</term>
<term>Proc</term>
<term>Protein synthesis</term>
<term>RNA, Antisense</term>
<term>Rnase cleavage</term>
<term>Second injection</term>
<term>Shuttleworth</term>
<term>Small proportion</term>
<term>Unfertihsed eggs</term>
<term>Unfertilised</term>
<term>Unfertilised eggs</term>
<term>Vegetal</term>
<term>Vegetal pole</term>
<term>Xenopus</term>
<term>Xenopus oocytes</term>
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<term>Animaux</term>
<term>Cinétique</term>
<term>Femelle</term>
<term>Fécondation</term>
<term>Sondes oligonucléotidiques</term>
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<front><div type="abstract" xml:lang="en">Abstract: We have investigated the effect of specific antisense oligodeoxynucleotides (oligos) on endogenous histone H4 mRNA in Xenopus oocytes, eggs and embryos. In unfertilised eggs and non-matured oocytes, one 20-mer oligo (H4-1) mediated the RNAse H-like cleavage of up to 95% of H4 mRNA (which included polysomal mRNA), and cleavage was still obtained when the size of the oligo was reduced to a 10-mer; no cleavage was observed with 6- and 8-mers. The residual uncleaved mRNA appeared to be completely inaccessible to H4-1 since a second injection caused no further cleavage. A second 20-mer (H4-2) directed against a different region of H4 mRNA was much less effective (<5% cleavage). In fertilised embryos, injections of H4-1 and an oligo directed against the localised Vg1 mRNA caused less cleavage than in oocytes and also showed signs of inducing localised, non-specific mRNA cleavage. However we have been able to prepare fertilised embryos devoid of Vgl mRNA by maturing and fertilising oligo-injected oocytes in vitro.</div>
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Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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