MersV1, PubMed, Corpus, bibRecord, 002A49

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos.

Identifieur interne : 002A49 ( PubMed/Corpus ); précédent : 002A48; suivant : 002A50

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos.

Auteurs : J. Shuttleworth ; G. Matthews ; L. Dale ; C. Baker ; A. Colman

Source :

Gene [ 0378-1119 ] ; 1988.

RBID : pubmed:2468567

English descriptors

KwdEn :
- Animals, Embryo, Nonmammalian (metabolism), Female, Fertilization, Histones (genetics), Kinetics, Oligonucleotide Probes, Oocytes (metabolism), RNA (genetics), RNA, Antisense, RNA, Messenger (antagonists & inhibitors), RNA, Messenger (genetics), Xenopus.
MESH :
- chemical , antagonists & inhibitors : RNA, Messenger.
- chemical , genetics : Histones, RNA, RNA, Messenger.
- metabolism : Embryo, Nonmammalian, Oocytes.
- Animals, Female, Fertilization, Kinetics, Oligonucleotide Probes, RNA, Antisense, Xenopus.

Abstract

We have investigated the effect of specific antisense oligodeoxynucleotides (oligos) on endogenous histone H4 mRNA in Xenopus oocytes, eggs and embryos. In unfertilised eggs and non-matured oocytes, one 20-mer oligo (H4-1) mediated the RNAse H-like cleavage of up to 95% of H4 mRNA (which included polysomal mRNA), and cleavage was still obtained when the size of the oligo was reduced to a 10-mer; no cleavage was observed with 6- and 8-mers. The residual uncleaved mRNA appeared to be completely inaccessible to H4-1 since a second injection caused no further cleavage. A second 20-mer (H4-2) directed against a different region of H4 mRNA was much less effective (less than 5% cleavage). In fertilised embryos, injections of H4-1 and an oligo directed against the localised Vg1 mRNA caused less cleavage than in oocytes and also showed signs of inducing localised, non-specific mRNA cleavage. However we have been able to prepare fertilised embryos devoid of Vg1 mRNA by maturing and fertilising oligo-injected oocytes in vitro.

DOI: 10.1016/0378-1119(88)90152-7
PubMed: 2468567

Links to Exploration step

pubmed:2468567

Le document en format XML

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<author><name sortKey="Shuttleworth, J" sort="Shuttleworth, J" uniqKey="Shuttleworth J" first="J" last="Shuttleworth">J. Shuttleworth</name>
<affiliation><nlm:affiliation>Biochemistry Department, University of Birmingham, U.K.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Matthews, G" sort="Matthews, G" uniqKey="Matthews G" first="G" last="Matthews">G. Matthews</name>
</author>
<author><name sortKey="Dale, L" sort="Dale, L" uniqKey="Dale L" first="L" last="Dale">L. Dale</name>
</author>
<author><name sortKey="Baker, C" sort="Baker, C" uniqKey="Baker C" first="C" last="Baker">C. Baker</name>
</author>
<author><name sortKey="Colman, A" sort="Colman, A" uniqKey="Colman A" first="A" last="Colman">A. Colman</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos.</title>
<author><name sortKey="Shuttleworth, J" sort="Shuttleworth, J" uniqKey="Shuttleworth J" first="J" last="Shuttleworth">J. Shuttleworth</name>
<affiliation><nlm:affiliation>Biochemistry Department, University of Birmingham, U.K.</nlm:affiliation>
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<author><name sortKey="Matthews, G" sort="Matthews, G" uniqKey="Matthews G" first="G" last="Matthews">G. Matthews</name>
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<author><name sortKey="Dale, L" sort="Dale, L" uniqKey="Dale L" first="L" last="Dale">L. Dale</name>
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<author><name sortKey="Baker, C" sort="Baker, C" uniqKey="Baker C" first="C" last="Baker">C. Baker</name>
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<author><name sortKey="Colman, A" sort="Colman, A" uniqKey="Colman A" first="A" last="Colman">A. Colman</name>
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<series><title level="j">Gene</title>
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<term>Embryo, Nonmammalian (metabolism)</term>
<term>Female</term>
<term>Fertilization</term>
<term>Histones (genetics)</term>
<term>Kinetics</term>
<term>Oligonucleotide Probes</term>
<term>Oocytes (metabolism)</term>
<term>RNA (genetics)</term>
<term>RNA, Antisense</term>
<term>RNA, Messenger (antagonists & inhibitors)</term>
<term>RNA, Messenger (genetics)</term>
<term>Xenopus</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Histones</term>
<term>RNA</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Embryo, Nonmammalian</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Female</term>
<term>Fertilization</term>
<term>Kinetics</term>
<term>Oligonucleotide Probes</term>
<term>RNA, Antisense</term>
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<front><div type="abstract" xml:lang="en">We have investigated the effect of specific antisense oligodeoxynucleotides (oligos) on endogenous histone H4 mRNA in Xenopus oocytes, eggs and embryos. In unfertilised eggs and non-matured oocytes, one 20-mer oligo (H4-1) mediated the RNAse H-like cleavage of up to 95% of H4 mRNA (which included polysomal mRNA), and cleavage was still obtained when the size of the oligo was reduced to a 10-mer; no cleavage was observed with 6- and 8-mers. The residual uncleaved mRNA appeared to be completely inaccessible to H4-1 since a second injection caused no further cleavage. A second 20-mer (H4-2) directed against a different region of H4 mRNA was much less effective (less than 5% cleavage). In fertilised embryos, injections of H4-1 and an oligo directed against the localised Vg1 mRNA caused less cleavage than in oocytes and also showed signs of inducing localised, non-specific mRNA cleavage. However we have been able to prepare fertilised embryos devoid of Vg1 mRNA by maturing and fertilising oligo-injected oocytes in vitro.</div>
</front>
</TEI>
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<DateCompleted><Year>1989</Year>
<Month>06</Month>
<Day>05</Day>
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<DateRevised><Year>2019</Year>
<Month>07</Month>
<Day>07</Day>
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<Issue>1-2</Issue>
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<Month>Dec</Month>
<Day>10</Day>
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<Title>Gene</Title>
<ISOAbbreviation>Gene</ISOAbbreviation>
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<ArticleTitle>Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos.</ArticleTitle>
<Pagination><MedlinePgn>267-75</MedlinePgn>
</Pagination>
<Abstract><AbstractText>We have investigated the effect of specific antisense oligodeoxynucleotides (oligos) on endogenous histone H4 mRNA in Xenopus oocytes, eggs and embryos. In unfertilised eggs and non-matured oocytes, one 20-mer oligo (H4-1) mediated the RNAse H-like cleavage of up to 95% of H4 mRNA (which included polysomal mRNA), and cleavage was still obtained when the size of the oligo was reduced to a 10-mer; no cleavage was observed with 6- and 8-mers. The residual uncleaved mRNA appeared to be completely inaccessible to H4-1 since a second injection caused no further cleavage. A second 20-mer (H4-2) directed against a different region of H4 mRNA was much less effective (less than 5% cleavage). In fertilised embryos, injections of H4-1 and an oligo directed against the localised Vg1 mRNA caused less cleavage than in oocytes and also showed signs of inducing localised, non-specific mRNA cleavage. However we have been able to prepare fertilised embryos devoid of Vg1 mRNA by maturing and fertilising oligo-injected oocytes in vitro.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shuttleworth</LastName>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos.

Antisense oligodeoxyribonucleotide-directed cleavage of maternal mRNA in Xenopus oocytes and embryos.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki