Biosynthesis of reovirus-specified polypeptides: Effect of point mutation of the sequences flanking the 5′-proximal AUG initiator codons of the reovirus S1 and S4 genes on the efficiency of mRNA translation
Identifieur interne : 004C45 ( Main/Exploration ); précédent : 004C44; suivant : 004C46Biosynthesis of reovirus-specified polypeptides: Effect of point mutation of the sequences flanking the 5′-proximal AUG initiator codons of the reovirus S1 and S4 genes on the efficiency of mRNA translation
Auteurs : Susan M. Munemitsu [États-Unis] ; Charles E. Samuel [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1988.
English descriptors
- Teeft :
Abstract
Abstract: The effect on translation of site-directed nucleotide substitutions around the 5′-proximal AUG initiation codon of the reovirus s1 mRNA specifying polypeptide σ1, and the reovirus s4 mRNA specifying polypeptide σ3 was examined. The efficiency of synthesis of the S1-encoded σ1, polypeptide and the S4-encoded σ3 polypeptide was analyzed in transfected simian COS cells. Mutant s1 mRNAs possessing either GCU AUG G or GCA AUG G sequences surrounding the 5′-proximal σ1, AUG were translated with an efficiency comparable to that of the wild-type s1 mRNA which possesses the flanking sequence CCU AUG G. Mutant s4 mRNAs possessing either CCU AUG G or CCA AUG G sequences surrounding the 5′-proximal σ3 AUG were translated with an efficiency comparable to that of wild-type s4 mRNA which possesses the flanking sequence GCA AUG G. The s4 mRNAs, both wild-type and mutant, were translated in vivo about five times more efficiently than the s1 mRNAs, both wild-type and mutant. These results suggest that nucleotide positions other than the −3, −2, −1, and +4 positions relative to the 5′-proximal initiator AUG, where the A is +1, play a dominant role in determining the efficiency of translation of these two reovirus mRNAs in vivo.
Url:
DOI: 10.1016/0042-6822(88)90309-1
Affiliations:
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Munemitsu</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Section of Biochemistry and Molecular Biology, Department of Biological Sciences, University of California, Santa Barbara, California 9310</wicri:regionArea><wicri:noRegion>California 9310</wicri:noRegion></affiliation></author><author><name sortKey="Samuel, Charles E" sort="Samuel, Charles E" uniqKey="Samuel C" first="Charles E." last="Samuel">Charles E. Samuel</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Section of Biochemistry and Molecular Biology, Department of Biological Sciences, University of California, Santa Barbara, California 9310</wicri:regionArea><wicri:noRegion>California 9310</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988">1988</date><biblScope unit="volume">163</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="643">643</biblScope><biblScope unit="page" to="646">646</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Codon</term><term>Dominant effect</term><term>Initiation codon</term><term>Kozak</term><term>Mrna</term><term>Munemitsu</term><term>Mutant</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide positions</term><term>Polypeptide</term><term>Psvs1</term><term>Psvs4</term><term>Reovirus</term><term>Reovirus mrnas</term><term>Reovirus polypeptides</term><term>Scanning model</term><term>Transfected</term><term>Transfected cells</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The effect on translation of site-directed nucleotide substitutions around the 5′-proximal AUG initiation codon of the reovirus s1 mRNA specifying polypeptide σ1, and the reovirus s4 mRNA specifying polypeptide σ3 was examined. The efficiency of synthesis of the S1-encoded σ1, polypeptide and the S4-encoded σ3 polypeptide was analyzed in transfected simian COS cells. Mutant s1 mRNAs possessing either GCU AUG G or GCA AUG G sequences surrounding the 5′-proximal σ1, AUG were translated with an efficiency comparable to that of the wild-type s1 mRNA which possesses the flanking sequence CCU AUG G. Mutant s4 mRNAs possessing either CCU AUG G or CCA AUG G sequences surrounding the 5′-proximal σ3 AUG were translated with an efficiency comparable to that of wild-type s4 mRNA which possesses the flanking sequence GCA AUG G. The s4 mRNAs, both wild-type and mutant, were translated in vivo about five times more efficiently than the s1 mRNAs, both wild-type and mutant. These results suggest that nucleotide positions other than the −3, −2, −1, and +4 positions relative to the 5′-proximal initiator AUG, where the A is +1, play a dominant role in determining the efficiency of translation of these two reovirus mRNAs in vivo.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Munemitsu, Susan M" sort="Munemitsu, Susan M" uniqKey="Munemitsu S" first="Susan M." last="Munemitsu">Susan M. Munemitsu</name></noRegion><name sortKey="Samuel, Charles E" sort="Samuel, Charles E" uniqKey="Samuel C" first="Charles E." last="Samuel">Charles E. Samuel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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