Downregulation of Msx-2 expression results in chondrogenesis in the medial region of the avian mandible.
Identifieur interne : 003E27 ( Main/Exploration ); précédent : 003E26; suivant : 003E28Downregulation of Msx-2 expression results in chondrogenesis in the medial region of the avian mandible.
Auteurs : M. Mina [États-Unis] ; J. Gluhak ; B. RodgersSource :
- Connective tissue research [ 0300-8207 ] ; 1996.
Descripteurs français
- KwdFr :
- ARN messager (analyse), Animaux, Cartilage (), Cartilage (embryologie), Cartilage (métabolisme), Embryon de poulet, Expression des gènes, Mandibule (), Mandibule (embryologie), Mandibule (métabolisme), Oligonucléotides antisens (pharmacologie), Protéines de liaison à l'ADN (génétique), Protéines à homéodomaine, Régulation négative, Techniques de culture d'organes.
- MESH :
- analyse : ARN messager.
- embryologie : Cartilage, Mandibule.
- génétique : Protéines de liaison à l'ADN.
- métabolisme : Cartilage, Mandibule.
- pharmacologie : Oligonucléotides antisens.
- Animaux, Cartilage, Embryon de poulet, Expression des gènes, Mandibule, Protéines à homéodomaine, Régulation négative, Techniques de culture d'organes.
English descriptors
- KwdEn :
- Animals, Cartilage (drug effects), Cartilage (embryology), Cartilage (metabolism), Chick Embryo, DNA-Binding Proteins (genetics), Down-Regulation, Gene Expression, Homeodomain Proteins, Mandible (drug effects), Mandible (embryology), Mandible (metabolism), Oligonucleotides, Antisense (pharmacology), Organ Culture Techniques, RNA, Messenger (analysis).
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : DNA-Binding Proteins.
- drug effects : Cartilage, Mandible.
- embryology : Cartilage, Mandible.
- metabolism : Cartilage, Mandible.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Animals, Chick Embryo, Down-Regulation, Gene Expression, Homeodomain Proteins, Organ Culture Techniques.
Abstract
Homeobox-containing genes are thought to be regulators of pattern formation during vertebrate development. We have previously characterized regions in the developing chick mandible expressing either Msx-1 or Msx-2 in terms of their chondrogenic potential, rates of cell proliferation, and their correlation with regions of cell death. These experiments suggest that Msx-1 may be involved in outgrowth of the mandibular arch, and Msx-2 may be involved in delineating the non-chondrogenic region of the medial part of the mandibular arch. To further examine the possibility that expression of Msx-2 may be involved in preventing chondrogenesis in the medial region, mandibular arch explants from stage 23 chick embryos were cultured for 4 days in media in the absence of serum but in the presence of 20-30 microM Msx-2 sense or antisense oligonucleotides (18 mers). In explants grown in either control media or with the sense oligonucleotide two rods of cartilage separated by a cartilage free area located in the medial region of the mandible were formed. In explants treated with Msx-2 antisense oligonucleotide cartilage formation was observed in the medial region of the mandible resulting in the fusion of the two bilateral rods at the midline. These results suggest a negative relationship between Msx-2 expression and chondrogenesis in the medial region of the developing mandible.
DOI: 10.3109/03008209609029177
PubMed: 9084645
Affiliations:
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Le document en format XML
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Mina</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pediatric Dentistry, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030, USA. Mina@nsol.uchc.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pediatric Dentistry, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030</wicri:regionArea><wicri:noRegion>Farmington 06030</wicri:noRegion></affiliation></author><author><name sortKey="Gluhak, J" sort="Gluhak, J" uniqKey="Gluhak J" first="J" last="Gluhak">J. Gluhak</name></author><author><name sortKey="Rodgers, B" sort="Rodgers, B" uniqKey="Rodgers B" first="B" last="Rodgers">B. Rodgers</name></author></analytic><series><title level="j">Connective tissue research</title><idno type="ISSN">0300-8207</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cartilage (drug effects)</term><term>Cartilage (embryology)</term><term>Cartilage (metabolism)</term><term>Chick Embryo</term><term>DNA-Binding Proteins (genetics)</term><term>Down-Regulation</term><term>Gene Expression</term><term>Homeodomain Proteins</term><term>Mandible (drug effects)</term><term>Mandible (embryology)</term><term>Mandible (metabolism)</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>Organ Culture Techniques</term><term>RNA, Messenger (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (analyse)</term><term>Animaux</term><term>Cartilage ()</term><term>Cartilage (embryologie)</term><term>Cartilage (métabolisme)</term><term>Embryon de poulet</term><term>Expression des gènes</term><term>Mandibule ()</term><term>Mandibule (embryologie)</term><term>Mandibule (métabolisme)</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines à homéodomaine</term><term>Régulation négative</term><term>Techniques de culture d'organes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA-Binding Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cartilage</term><term>Mandible</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Cartilage</term><term>Mandibule</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Cartilage</term><term>Mandible</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de liaison à l'ADN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cartilage</term><term>Mandible</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cartilage</term><term>Mandibule</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Down-Regulation</term><term>Gene Expression</term><term>Homeodomain Proteins</term><term>Organ Culture Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartilage</term><term>Embryon de poulet</term><term>Expression des gènes</term><term>Mandibule</term><term>Protéines à homéodomaine</term><term>Régulation négative</term><term>Techniques de culture d'organes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Homeobox-containing genes are thought to be regulators of pattern formation during vertebrate development. We have previously characterized regions in the developing chick mandible expressing either Msx-1 or Msx-2 in terms of their chondrogenic potential, rates of cell proliferation, and their correlation with regions of cell death. These experiments suggest that Msx-1 may be involved in outgrowth of the mandibular arch, and Msx-2 may be involved in delineating the non-chondrogenic region of the medial part of the mandibular arch. To further examine the possibility that expression of Msx-2 may be involved in preventing chondrogenesis in the medial region, mandibular arch explants from stage 23 chick embryos were cultured for 4 days in media in the absence of serum but in the presence of 20-30 microM Msx-2 sense or antisense oligonucleotides (18 mers). In explants grown in either control media or with the sense oligonucleotide two rods of cartilage separated by a cartilage free area located in the medial region of the mandible were formed. In explants treated with Msx-2 antisense oligonucleotide cartilage formation was observed in the medial region of the mandible resulting in the fusion of the two bilateral rods at the midline. These results suggest a negative relationship between Msx-2 expression and chondrogenesis in the medial region of the developing mandible.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Gluhak, J" sort="Gluhak, J" uniqKey="Gluhak J" first="J" last="Gluhak">J. Gluhak</name><name sortKey="Rodgers, B" sort="Rodgers, B" uniqKey="Rodgers B" first="B" last="Rodgers">B. Rodgers</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Mina, M" sort="Mina, M" uniqKey="Mina M" first="M" last="Mina">M. Mina</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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